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Dopamine sensor based on nitrogen doped graphene as well as preparation and application thereof

A technology of nitrogen-doped graphene and dopamine, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve problems affecting applications, high prices, and biological activity effects, and achieve low detection limits, simple preparation, and stable performance.

Pending Publication Date: 2018-11-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the detection method of dopamine, the preparation of biosensors relying on enzymes or antibodies specifically recognized by dopamine to prepare biosensors, in addition to being expensive, there are other deficiencies. Taking enzymes as an example, during storage and use, the environment The temperature, pH and other aspects have a serious impact on its biological activity, which will affect its application. It is of great significance to choose non-enzymatic and cheap substances that have catalytic activity for dopamine to prepare dopamine electrochemical sensors.

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  • Dopamine sensor based on nitrogen doped graphene as well as preparation and application thereof
  • Dopamine sensor based on nitrogen doped graphene as well as preparation and application thereof
  • Dopamine sensor based on nitrogen doped graphene as well as preparation and application thereof

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Embodiment 1

[0039] A preparation method of a dopamine sensor based on nitrogen-doped graphene, comprising the following steps:

[0040] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0041](2) Ultrasonically peel 100mg of graphene oxide in 50mL of deionized water for 4h (ultrasonic power is 100W), add 0.25mL of aniline, stir well and continue ultrasonication for 30min; under low temperature stirring (8°C), add 5mL containing 0.625g of ammonium persulfate (APS) 0.5M HCl (5mL of 0.5M HCl+0.625g ammonium persulfate), continue to stir at low temperature for 6 hours, then transfer to a hydrothermal reaction kettle, conduct a hydrothermal r...

Embodiment 2

[0047] A preparation method of a dopamine sensor based on nitrogen-doped graphene, comprising the following steps:

[0048] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0049] (2) Add 100 mg of graphene oxide into 50 mL of deionized water and ultrasonically peel for 4 hours, add 0.5 mL of aniline, stir evenly and continue ultrasonication for 30 min, add 5 mL of 0.5 M solution containing 1.25 g of ammonium persulfate (APS) under low temperature stirring (8°C). HCl, continue to stir at low temperature for 6 hours, then transfer to the reaction kettle, heat at 180°C for 16 hours, filter, wash with distilled water several ti...

Embodiment 3

[0058] A preparation method of a dopamine sensor based on nitrogen-doped graphene, comprising the following steps:

[0059] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;

[0060] (2) Add 100 mg of graphene oxide into 50 mL of deionized water for ultrasonic peeling for 4 h, add 1 mL of aniline, stir well and continue ultrasonication for 30 min, then add 5 mL of 0.5 M HCl containing 2.5 g of ammonium persulfate (APS) under low temperature stirring (8°C) , continue to stir at low temperature for 6 hours, then transfer to the reaction kettle, heat at 180°C for 16 hours, filter, wash with distilled water several times, dry in va...

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Abstract

The invention belongs to the technical field of an electrochemical sensor, and discloses a dopamine sensor based on nitrogen doped graphene as well as preparation and application thereof. The sensor consists of a reference electrode, a counter electrode and a modified work electrode, wherein the modified work electrode consists of a work electrode and a substrate recognition membrane cured at thesurface of the work electrode; the substrate recognition membrane is mainly prepared from nitrogen doped graphene composite materials and perfluorinated sulfonic acid resin. The invention also discloses a preparation method of the sensor. The sensor has high selectivity, reproducibility and stability; the dopamine can be accurately detected; the antijamming capability is high; meanwhile, the detection range is wider; the detection limit is lower. The method is simple; the cost is low. The prepared sensor is used for detecting the dopamine.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, and in particular relates to a sensor for detecting dopamine based on nitrogen-doped graphene, a preparation method and application thereof. Background technique [0002] As one of the most important catecholamines in the body, dopamine (hereinafter referred to as DA) is a mediator that transmits information between neurons, that is, a neurotransmitter, and its concentration range is about 10 -7 ~10 -3 mol / L. DA plays an indispensable role in the functions of the central nervous system, cardiovascular system, renal system and hormone secretion system, and the abnormal concentration of DA can affect or even cause Parkinson's disease, HIV infection, schizophrenia, hyperuricemia syndrome and rheumatoid arthritis among many other diseases. At present, there are many methods for detecting blood dopamine, such as gas-liquid chromatography-mass spectrometry, temperature measurement, m...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 黄彩弯朴金花林芷琪王雯童
Owner SOUTH CHINA UNIV OF TECH
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