Antigen epitope, antibody, identification method and application of Newcastle disease virus matrix protein

A technology for Newcastle disease virus and antigenic epitope is applied in the field of antigenic epitope of Newcastle disease virus matrix protein, which can solve the problems of less disclosure of technology and achieve the effect of simple and convenient identification system.

Inactive Publication Date: 2021-03-30
NORTHWEST A & F UNIV
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  • Abstract
  • Description
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Problems solved by technology

NDV has only one serotype, and the accurate epitope of M protein can be used as a biological marker to lay the foundation for the diagnosis of Newcastle disease and the development of marked vaccines, but there are few related technologies disclosed at present

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  • Antigen epitope, antibody, identification method and application of Newcastle disease virus matrix protein
  • Antigen epitope, antibody, identification method and application of Newcastle disease virus matrix protein
  • Antigen epitope, antibody, identification method and application of Newcastle disease virus matrix protein

Examples

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Embodiment

[0047] A method for identifying the dominant antigenic epitope of Newcastle disease virus LaSota strain M protein B cells is as follows:

[0048] Step 1: Use DNAStar Protean software to analyze the secondary structure of NDV LaSota strain M protein (amino acid sequence is SEQ ID NO. 7)); use online database Immune Epitope Database (IEDB), ABCpred, Bcepred to comprehensively analyze LaSota strain M protein B cell epitopes were predicted; using the following 7 prediction schemes: hydrophilic scheme, protruding surface and turning scheme, surface accessibility scheme, flexibility scheme, polarity scheme, antigenicity scheme; as shown in Figure 1, M The protein has abundant α helix, β sheet and turn structure, strong hydrophobicity, high surface accessibility and antigen index; and there are about 9 linear epitopes with high score and stable existence in the M protein, among which The locations are as follows: 28-39, 48-56, 77-85, 118-126, 157-169, 223-233, 244-253, 296-311 and 34...

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Abstract

The invention discloses antigenic epitopes, antibodies, identification methods and applications of Newcastle disease virus (NDV) matrix (M) proteins, and relates to the technical field of biology. Theantigenic epitopes of the NDV M proteins provided by the invention has a sequence 1 of 77MIDDKP82 and a sequence 2 of 354HTLAKYNPFK363. B cell dominant linear epitopes of the two NDV M proteins provided by the invention can be used for detection antigen of serological analysis of NDV, and can be used as markers for constructing marker vaccines of the NDV; sequences of the B cell dominant linear epitopes of the two M proteins provided by the invention has a relatively high conservative property in the M proteins of all genotypes of the NDV; and epitope regions 77-82 and 354-363 of the M proteins are applicable to all NDV strains.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to an antigenic epitope, antibody, identification method and application of a Newcastle disease virus matrix protein. Background technique [0002] Newcastle Disease (ND) is an acute and highly lethal infectious disease caused by Newcastle Disease Virus (NDV). NDV is a single-stranded negative-stranded RNA virus belonging to the genus Avian Paramyxovirus of the family Paramyxoviridae. The NDV genome is about 15.2kb in length, similar to other paramyxoviruses. Its genes encode nucleoprotein (NP), phosphoprotein (P), matrix protein (M), fusion protein (fusion protein) , F), 6 proteins including hemagglutinin-nueraminidase (HN) and large polymerase protein (RNA-dependent RNApolymerase or large ploymerase, L), the genome starts with 3'-NP-P-M-F- HN-L-5' sequence. [0003] Among them, the M gene has a nucleotide length of 1241bp and an open reading frame of 1095bp, encoding a total of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/125G01N33/68G01N33/569C07K16/10A61K39/17A61P31/14
CPCA61K39/00A61P31/14C07K14/005C07K16/1027C12N2760/18122G01N33/56983G01N33/6854G01N33/6878G01N2333/125G01N2469/10G01N2469/20
Inventor 萧飒毕友坤杨增岐陈鸿军王文彬金忠元
Owner NORTHWEST A & F UNIV
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