Method for dissolving low-grade phosphate rock powder by utilizing endophytic bacteria of myriophyllum spicatum

A technology of foxtail algae and endophyte, which is applied in the directions of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of single, low-grade phosphate rock, etc., and achieves a simple process, is conducive to reproduction, and improves dissolution efficiency. Effect

Inactive Publication Date: 2018-11-06
江口县旭辉生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although some good progress has been made in the research on the dissolution of medium and low-grade phosphate rock by microorganisms, there are still some deficiencies. , Research on the dissolution of medium and low-grade phosphate rocks by microbial flora that grows cooperatively with plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:

[0033] (1) Isolation and screening of endophytes:

[0034] a. Isolation of endophytic bacteria from P. spicaculata:

[0035] In the field, collect the intact and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 10 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 5 minutes, and then soak it in 1.2% ethanol solution for 5 minutes. Soak in sodium hypochlorite solution for 3 minutes, soak in 0.1% mercuric chloride solution for 2 minutes, and finally rinse with sterile water for 10 times and blot dry. Cut the plant into 1.5cm sections, cut them longitudinally, and stick the cut surface on the beef Paste the surface of the peptone solid medium, and place the prepared plate in a constant temperature incubator at 25°C for 2 weeks to obtain bacterial cells;

[0036] b....

Embodiment 2

[0046] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:

[0047] (1) Isolation and screening of endophytes:

[0048] a. Isolation of endophytic bacteria from P. spicaculata:

[0049] In the wild, collect the complete and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 8 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 3 minutes, and then soak it in 1% ethanol solution for 3 minutes. Soak in sodium hypochlorite solution for 2 minutes, soak in 0.1% mercuric chloride solution for 1 minute, and finally rinse with sterile water for 8 times and blot dry. Cut the plant into 1 cm sections, cut them longitudinally, and stick the cut surface on the beef paste On the surface of peptone solid medium, place the prepared plate in a constant temperature incubator at 25°C for 2 weeks to obtain bacteria;

[0050] b. Screening of endop...

Embodiment 3

[0060] A method for dissolving low-grade phosphate rock powder by endophytic bacteria of P. spicaculata, comprising the steps of:

[0061] (1) Isolation and screening of endophytes:

[0062] a. Isolation of endophytic bacteria from P. spicaculata:

[0063] In the wild, collect the complete and vigorously grown Trichosanthes spicaris, rinse it with tap water, and then rinse it with sterile water 9 times, blot it dry with sterile filter paper, soak it in 70% ethanol solution for 3 minutes, and then soak it in 0.8% ethanol solution for 3 minutes. Soak in sodium hypochlorite solution for 2 minutes, soak in 0.1% mercuric chloride solution for 1 minute, and finally rinse with sterile water for 9 times and blot dry. Cut the plant into 0.8 cm sections, cut them longitudinally, and stick the cut surface on the beef On the surface of the peptone solid medium, place the prepared plate in a constant temperature incubator at 25°C for 13 days to obtain the bacteria;

[0064] b. Screening ...

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PUM

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Abstract

The invention discloses a method for dissolving low-grade phosphate rock powder by utilizing endophytic bacteria of myriophyllum spicatum, and relates to the technical field of phosphate rock mining and processing. The method comprises the following steps: firstly, separating the endophytic bacteria of myriophyllum spicatum, then screening and expanding propagation, obtaining highly active phosphorus-solubilizing endophytic bacteria, then mixing the endophytic bacteria with a culture medium and the low-grade phosphate rock powder for culture, and utilizing organic acids, enzymes and other substances secreted by the endophytic bacteria of myriophyllum spicatum to convert insoluble phosphorus into soluble phosphorus. The method is low in energy consumption and excellent in phosphorus solubilizing effect, and no waste gas that pollutes the environment is generated, and the method is of great practical value.

Description

technical field [0001] The invention relates to the technical field of phosphate rock mining and processing, in particular to a method for dissolving low-grade phosphate rock powder by using endophytic bacteria of P. spicanus. Background technique [0002] my country is a country rich in phosphate rock resources, but most of them are medium and low-grade phosphate rock. Among the proven reserves of phosphate rock in my country, the average grade of phosphate rock is about 17%, and the rich ore with an ore grade greater than 30% does not exceed 10% of the total reserves. The middle and low-grade phosphate rock with an ore grade of less than 30% has fine particle size, complex mineral composition and high impurity content. These factors determine that the production cost is high and the economic benefit is low when the traditional wet or thermal phosphate fertilizer production process is used to process these low-grade phosphate rocks. [0003] At present, most phosphate roc...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/02C05B17/00
CPCC05B17/00C12N1/00C12N1/02
Inventor 杨茂林
Owner 江口县旭辉生态农业科技有限公司
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