Preparation method of brain protein hydrolyzate injection

Abstract

Brain protein hydrolysate injection is a sterilized preparation prepared through enzymatic hydrolysis of a healthy pig brain. The brain protein hydrolyzate injection comprises about 16 types of free amino acids and a small amount of peptides, and is used for the improvement of symptoms of craniocerebral trauma, sequelas of cerebrovascular diseases accompanied with hypomnesia and an attention deficit disorder. The brain protein hydrolyzate injection has an auxiliary improvement effect on brain dysfunction, and is also used in patients with protein deficiency and neurasthenia and cases of protein digestion and absorption disorder. The invention introduces a preparation method of the brain protein hydrolyzate injection. The preparation method comprises the following steps: washing, homogenization, thermal change, pepsin hydrolysis, deep freezing, thawing, pancreatic enzymatic hydrolysis, thermal change, ultrafiltration, nanofiltration interception with the molecular weight of 300-1000, ultrafiltration again, dilution of a supplemental amino acid to a middle line, addition of sodium bisulfite for oxidation resistance, sterile filtration, potting and sterilization. The whole process iscomplete, simple and convenient, the refractive index is guaranteed, and the characters are qualified, so that products meet the national standard, especially that the injection can still maintain a light yellow color in a storage process.

Description

technical field [0001] The invention belongs to the technical field of medicine. Background technique [0002] Brain protein hydrolyzate injection is a sterilized aqueous solution prepared by enzymatic hydrolysis of healthy pig brains. Each 1ml contains 28.08-42.14mg of free amino acids and 5.49-6.71mg of total nitrogen (N). It is used for craniocerebral trauma , Sequelae of cerebrovascular disease accompanied by improvement of symptoms of memory loss and attention deficit disorder. It can help to improve brain dysfunction, and it is also used for protein deficiency and neurasthenia patients for cases of general protein digestion and absorption disorders. [0003] This variety was first imported from Austria, and later produced in China. There are many literature reports on its technological process, and its process is roughly the process of degreasing, hydrolysis, ultrafiltration, chromatography, and deployment of amino acids. But in practice, we cannot produce qualified...

AI Technical Summary

Problems solved by technology

But in practice, we cannot produce qualified products according to the reported process, mainly because the other substances in the standard [inspection] items are more than 15%, and the color, especially in the storage process, cannot meet the requirements of light yellow clear liquid

In addition, the degreasing step requires an explosion-proof workshop. On the one hand, it increases the cost, and on the other hand, it is really unnecessary in the process.

The chromatography process is not realistic in practice