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LAMP (Loop-Mediated Isothermal Amplification) detection primer pair for Seneca valley virus and detection method of Seneca valley virus

A detection method and detection primer technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/testing, etc., can solve the problem of no rapid diagnostic method

Active Publication Date: 2018-10-26
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no rapid diagnostic methods and commercial vaccines in my country

Method used

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  • LAMP (Loop-Mediated Isothermal Amplification) detection primer pair for Seneca valley virus and detection method of Seneca valley virus
  • LAMP (Loop-Mediated Isothermal Amplification) detection primer pair for Seneca valley virus and detection method of Seneca valley virus
  • LAMP (Loop-Mediated Isothermal Amplification) detection primer pair for Seneca valley virus and detection method of Seneca valley virus

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Embodiment 1

[0031] 1 Materials and methods

[0032] 1.1 Viruses, recombinant plasmids and disease materials

[0033] Viruses: SVV L16 strain, PRRSV, PCV2, porcine pseudorabies virus (PRV), swine fever virus (CSFV), porcine epidemic diarrhea virus (PEDV) were all isolated by the Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Store at -20°C.

[0034] Recombinant plasmid: pTOPO-SVV-A (containing SVV P1 gene fragment), the concentration is 500 ng·μL -1 , gene copy number is 1x10 9 copies μL -1 , constructed and provided by the Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, stored at -20°C.

[0035] Clinical samples: 10 samples of vesicles from the trotters with SVV clinical onset, collected by the Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, and stored at -20°C.

[0036] Viral Nucleic Acid Extraction

[0037] SVV, PRRSV, PEDV, and CSFV ...

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Abstract

The invention relates to the technical field of virus detection, in particular to an LAMP (Loop-Mediated Isothermal Amplification) detection primer pair for a Seneca valley virus and also relates to adetection method using the primer pair. The method is high in specificity, has the sensibility consistent with fluorescent quantitative PCR, is rapid and convenient, can be used for detection of SVVand has a better application prospect.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a pair of LAMP detection primers for Seneca Valley virus, and also to a detection method using the aforementioned primer pair. Background technique [0002] Swine Seneca Valley virus (SVV) is a new epidemic pathogen in swine herds at home and abroad recently, which poses a great threat to the pig industry. my country is a big pig raising country and also the largest pig production base, so once a new disease breaks out, if it cannot be stopped, it may have a huge impact on my country's pig industry and cause considerable economic losses. It is necessary to attach great importance to the epidemic dynamics of pathogens to prevent problems before they happen. Therefore, it is particularly important to establish a simple and effective detection method for new pathogens. [0003] Seneca Valley Virus (Seneca Valley Virus) is a positive single-stranded RNA virus belonging to...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2563/107
Inventor 姜平姜辰龙张日腾白娟
Owner NANJING AGRICULTURAL UNIVERSITY
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