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Method for high-density culture of endophytic bacteria of psidium guajava leaves

A technology of high-density culture and guava leaves, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., to achieve the effects of stable product performance, simple cultivation methods, and easy operation

Inactive Publication Date: 2018-10-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many endophytic strains capable of producing plant secondary metabolites have been isolated, almost none of them are used in production.

Method used

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  • Method for high-density culture of endophytic bacteria of psidium guajava leaves

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Effect test

Embodiment 1

[0033] Such as figure 1 Shown, a kind of method for cultivating guava leaf endophyte at high density comprises the following steps:

[0034] (1) Guava leaf pretreatment: pick fresh guava leaves from the guava tree, dry the guava leaves at 45°C for 12 hours, then grind them into fine powder, add sterile water, and filter through a 220 μm filter membrane Remove other miscellaneous bacteria other than specific endophytes;

[0035] (2) Activation and cultivation of endophytes: Inoculate endophytes isolated and screened from guava leaves that can produce terpenoids on solid microbial culture media for endophyte culture and activation, control the inoculum size and solid microbial culture The ratio of carbon source and nitrogen source in the base is: inoculum amount (10 7 cells): Carbon source (g): Nitrogen source (g) = 1.6:5:1.3, and the culture temperature was controlled at 28°C for 30 hours; wherein, the composition of the solid microbial culture medium included: peptone 6g / L, ...

Embodiment 2

[0041] Such as figure 1 Shown, a kind of method for cultivating guava leaf endophyte at high density comprises the following steps:

[0042] (1) Guava leaf pretreatment: pick fresh guava leaves from the guava tree, dry the guava leaves at 45°C for 18 hours, then grind them into fine powder, add sterile water, and filter through a 220 μm filter membrane Remove other miscellaneous bacteria other than specific endophytes;

[0043] (2) Activation and cultivation of endophytes: Inoculate endophytes isolated and screened from guava leaves that can produce terpenoids on solid microbial culture media for endophyte culture and activation, control the inoculum size and solid microbial culture The ratio of carbon source and nitrogen source in the base is: inoculum amount (10 7cells): Carbon source (g): Nitrogen source (g) = 1.6:5:1.3, and the culture temperature was controlled at 28°C for 24 hours; wherein, the composition of the solid microbial culture medium included: peptone 6g / L, y...

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Abstract

The invention discloses a method for high-density culture of endophytic bacteria of psidium guajava leaves. The method comprises the following steps: (1) drying the psidium guajava leaves, processingthe dried psidium guajava leaves into powder, and conducting sterilizing processing, so as to obtain pre-processed psidium guajava leaf powder; (2) activating endophytic bacteria; (3) inoculating a solid medium containing the pre-processed psidium guajava leaf powder obtained in the step (1) with the activated endophytic bacteria, and implementing culture, so as to obtain a co-culture product; (4)inoculating endophytic bacterium seed liquid which is cultivated in a liquid medium with the co-culture product obtained in the step (3); and (5) adding the endophytic bacterium seed liquid and a liquid medium containing a phosphorus source to a fermentation tank, and implementing high-density culture, so that high-density psidium guajava leaf endophytic bacteria are obtained. According to the method provided by the invention, by conducting high-density culture of the endophytic bacteria in an environment of the psidium guajava leaves, a great amount of metabolic products having bio-activitycan be generated through metabolism; and the method has the advantages of being stable in product performance, low in cost, environment-friendly and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for cultivating guava leaf endophytes. Background technique [0002] Guava is one of the best-selling high-quality fruits in South China, and its development prospect is good. Guava biological fruit is sweet and refreshing, sweet and juicy, pleasant aroma, unique flavor, rich in nutrients, and is very popular among consumers. In addition, guava leaves also have certain medicinal value. Guava leaves are widely used in Chinese and foreign folks to treat diseases such as diabetes and diarrhea. This is because guava leaves contain many phenolic components with novel structures. A large number of studies have shown that guava leaf phenols Such ingredients have the effects of lowering blood sugar, anti-oxidation, anti-cancer and anti-pathogenic microorganisms. However, because the current extraction process is not yet mature, it cannot effectively extract biologically active sub...

Claims

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Application Information

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IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 曾伟民周智广李交昆胡芳申丽黄干余润兰吴学玲刘元东吴晨晨李芳刘阿娟邱冠周
Owner CENT SOUTH UNIV
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