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Ophthalmic preparation containing small molecular caulis dendrobii polysaccharide as well as preparation method and application

A technology of dendrobium polysaccharide and ophthalmic preparation is applied in the field of preparation of ophthalmic preparations containing small molecule dendrobium polysaccharide, which can solve the problems of complex dry eye mechanism and the like, and achieve the effects of simple and easy preparation method and reliable curative effect.

Inactive Publication Date: 2018-10-12
南京润斛堂生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of dry eye syndrome itself is complex, and there are few pharmacological studies on hydrolyzed dendrobium polysaccharides at home and abroad, so the chemical substance basis of its efficacy in the treatment of dry eye disease needs further research

Method used

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  • Ophthalmic preparation containing small molecular caulis dendrobii polysaccharide as well as preparation method and application
  • Ophthalmic preparation containing small molecular caulis dendrobii polysaccharide as well as preparation method and application
  • Ophthalmic preparation containing small molecular caulis dendrobii polysaccharide as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of unhydrolyzed macromolecular polysaccharides from Dendrobium officinale

[0035] Take 5000g of dry product of Dendrobium candidum, crush it, add 30 times of water, heat and boil for extraction for 2 hours, extract twice, centrifuge to obtain a centrifuge, concentrate the centrifuge to 15L, add 4 times the volume of ethanol, that is, a total of 60L of ethanol, alcohol precipitation to precipitate polysaccharides, Centrifuge the polysaccharide to precipitate, and collect it to obtain the unhydrolyzed macromolecular Dendrobium officinale polysaccharide.

Embodiment 2

[0036] Example 2: Preparation of acid hydrolyzed small molecule Dendrobium officinale polysaccharide

[0037] Take part of the non-hydrolyzed macromolecular dendrobium officinale polysaccharide prepared in Example 1, add 1mol / L hydrochloric acid, heat in a water bath at 80°C, keep it warm for 1 hour, add NaOH solution to neutralize to neutral, use a dialysis membrane to dialyze out NaCl, and separate Precipitation of polysaccharides in the heart, that is, acid-hydrolyzed small-molecule Dendrobium officinale polysaccharides. Using the evaporative light scattering detector (ELSD), the acid-hydrolyzed small molecule Dendrobium officinale polysaccharide measured has a peak molecular weight of 41.42KDa and a molecular weight width of 29.83-75.25KDa.

Embodiment 3

[0038] Example 3: Preparation of enzymatically hydrolyzed small molecule Dendrobium officinale polysaccharides

[0039] Get part of the non-hydrolyzed macromolecular polysaccharides of Dendrobium candidum prepared in Example 1 to make a concentrated solution (the content is equivalent to every 1ml concentrated solution containing every 1g of Dendrobium candidum crude drug) to adjust the pH value of the solution to about PH6, and add polysaccharides equivalent to the content of Dendrobium candidum polysaccharides 1% pectinase and cellulase (1:1) are used to enzymatically hydrolyze the polysaccharide of Dendrobium officinale, the enzymatic hydrolysis temperature is about 40°C, and the enzymatic hydrolysis time is 1.5h. After the enzymatic hydrolysis is completed, boil to inactivate the enzyme, centrifuge, and centrifuge to precipitate to obtain the enzymatically hydrolyzed small molecule Dendrobium officinale polysaccharide. Using the evaporative light scattering detector (ELSD)...

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Abstract

The invention discloses an ophthalmic preparation containing small molecular caulis dendrobii polysaccharide as well as a preparation method and application. A main drug of the preparation is the small molecular caulis dendrobii polysaccharide obtained through hydrolysis. The molecular weight range of the small molecular caulis dendrobii polysaccharide is 25 to 75KDa. The small molecular caulis dendrobii polysaccharide can be prepared through acid hydrolysis or enzyme hydrolysis; a product obtained by the enzyme hydrolysis has a more excellent curative effect and has remarkable anti-wrinklingand anti-chloasma curative effects. The invention accidentally finds out that an unhydrolyzed caulis dendrobii polysaccharide group aggravates symptoms of dry eye syndrome, visual fatigue, under-eye dark circle and under-eye puffiness; the small molecular caulis dendrobii polysaccharide prepared by hydrolyzing the caulis dendrobii polysaccharide has a remarkable treatment effect.

Description

technical field [0001] The invention belongs to the field of ophthalmic preparations, and in particular relates to a preparation method and application of an ophthalmic preparation containing small-molecule dendrobium polysaccharides. Background technique [0002] Dry eye is a common chronic disease of the ocular surface in clinical practice. Abnormal tear quality or quantity or abnormal dynamics caused by any reason will lead to decreased stability of tear film, accompanied by ocular discomfort and / or multiple pathological changes in ocular surface tissue. The general term of this disease, also known as keratoconjunctival sicca. Patients often have eye dryness and foreign body sensation, other symptoms include burning sensation, itching, fear of wind, photophobia, red pain, blurred vision, easy fatigue, secretion of sticky and filamentous secretions, etc., and are very sensitive to external stimuli; Sometimes the eyes are too dry and there is insufficient tear fluid, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P27/02A61K8/9794A61K8/73A61Q19/00A61Q19/02A61K36/8984
Inventor 李定祥熊秀娟朱慧慧
Owner 南京润斛堂生物科技有限公司
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