Simple extraction method of coumarin compounds in angelica dahurica and application thereof

A technology of coumarins and extraction methods, applied in the field of medicine, can solve problems such as allergic reactions, degranulation reactions, painful life threats, etc., and achieve the effect of simple extraction methods

Inactive Publication Date: 2018-10-09
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Clinically, anaphylactoid reactions may occur when patients take the drug for the first time, which not only brings huge economic burden to patients, but also causes new pain and even life-threatening
Studies have found that human MrgprX2 receptors can mediate anaphylactoid reactions. When MrgprX2 receptors are stimulated by drugs, the concentration of calcium ions in mast cells can be increased to trigger degranulation reactions, which in turn lead to anaphylactoid reactions.

Method used

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  • Simple extraction method of coumarin compounds in angelica dahurica and application thereof
  • Simple extraction method of coumarin compounds in angelica dahurica and application thereof
  • Simple extraction method of coumarin compounds in angelica dahurica and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Experimental method

[0024] The decoction slices of Angelica dahurica were crushed into coarse powder, and 50 g of Angelica dahurica coarse powder was weighed, placed in a stoppered Erlenmeyer flask, 150 mL of methanol was added, and ultrasonic extraction was performed for 30 minutes under the condition of ultrasonic power of 200 W and frequency of 40 kHz. The filtrate was filtered, and the solvent (methanol) was evaporated with a rotary evaporator to obtain the coumarin extract. Additionally, methanol can be recycled.

Embodiment 2

[0026] 1. Experimental method

[0027] (1) Histamine release

[0028] KU812 cells were seeded in 96-well plates at 30,000 cells / well and cultured overnight. After the 96-well plate was centrifuged at 1500rmp for 5min, the original culture medium was sucked out, and 90ul TM buffer (negative control group), 30ug / ml C48 / 80 solution of the drug (administration group) or 30ug / ml C48 / 80 solution (administration group) were added respectively. Positive control group) was incubated in an incubator at 37°C for 30 minutes, then centrifuged at 1500rmp for 5 minutes, and 50ul of each well was pipetted into ep tubes. Add 100ul of deuterated histamine acetonitrile solution, vortex, centrifuge at 12000×g for 10min, take the supernatant, and measure the content of histamine by HPLC-MS-MS.

[0029] 2. Experimental results

[0030] The data are expressed as mean ± standard error (mean ± SEM), and the graphs are drawn using Graphpad Prism 5.0 software.

[0031] figure 1 It is the HPLC chrom...

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Abstract

The invention discloses a simple extraction method of coumarin compounds in angelica dahurica. Angelica dahurica is extracted through methanol; an extractive mainly comprises coumarin compounds, suchas imperatorin and isoimperatorin; an antagonistic effect on histamine release of human mast cell KU812 caused by compounds 48/80 by the angelica dahurica extractive, imperatorin, isoimperatorin and phellopterin is tested. Results show that the extraction technology is simple and effective, and the angelica dahurica extractive, imperatorin, isoimperatorin and phellopterin can effectively antagonize histamine release of the human mast cell KU812 caused by the compounds 48/80. The method provides a novel method and idea on the development of novel antiallergic drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a simple extraction method and application of coumarin compounds in Angelica dahurica. Background technique [0002] The chemical composition of Angelica dahurica is complex, mainly including coumarins, alkaloids, volatile oil, sitosterol, etc. Pharmacological studies have shown that Angelica dahurica has antipyretic, analgesic and anti-inflammatory effects, and has important effects on cardiovascular, central nervous system, smooth muscle, anti-tumor, and photosensitivity. significant effect. The good curative effect of coumarin has made the research on the extraction method of coumarin has been well developed, but the polarity of active ingredients in Angelica dahurica is relatively close, so the extraction and preparation of coumarin substances in Angelica dahurica requires cumbersome steps. [0003] Drug-like allergic reactions are caused by drugs directly stimulating mast ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/232A61P37/08
CPCA61K36/232A61K2236/33A61P37/08
Inventor 贺怀贞张涛韩省力卢闻李维凤贺浪冲
Owner XI AN JIAOTONG UNIV
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