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Degradation/adsorption bacteria for sulfonamide antibiotics/heavy metal composite pollution and application thereof

A compound pollution and sulfonamide technology, applied in the field of environmental pollutant treatment, can solve problems such as heavy metal pollution and sulfonamide antibiotic pollution, and achieve the effects of reducing harm, good adsorption, and good degradation effect

Active Publication Date: 2018-10-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the existing sulfonamide antibiotic pollution and heavy metal pollution, especially the defects and deficiencies of the combined pollution treatment technology of the two, and to provide a microbial bacterium capable of efficiently degrading sulfa antibiotics and adsorbing heavy metals.

Method used

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  • Degradation/adsorption bacteria for sulfonamide antibiotics/heavy metal composite pollution and application thereof
  • Degradation/adsorption bacteria for sulfonamide antibiotics/heavy metal composite pollution and application thereof
  • Degradation/adsorption bacteria for sulfonamide antibiotics/heavy metal composite pollution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Separation and identification of sulfonamide antibiotics / heavy metal copper complex pollution degradation / adsorption bacteria

[0033] 1. Experimental materials

[0034] Nutrient medium: 5 g sodium chloride, 3 g beef extract, 10 g protein, 1000 mL distilled water, pH 7.0-7.2. For solid medium, add agar powder (bar) 15 g / L.

[0035] Inorganic salt medium: (NH 4 ) 2 SO 4 1 g, MgSO 4 ·7H 2 O 0.1 g, KH 2 PO 4 3 g, K 2 HPO 4 ·3H 2 O 7 g, sodium citrate 0.5 g, distilled water 1000 mL, pH 7.0-7.2.

[0036] Before the experiment, the medium was placed in a high-pressure steam sterilizer and sterilized at 121 °C for 30 min.

[0037] 2. Isolation and identification of strains

[0038] (1) Screening and domestication of resistant bacteria:

[0039]Microbial domestication proceeds in four stages. A contaminated soil sample from a livestock and poultry farm in the Pearl River Delta was collected. First, under aseptic conditions, the soil sample was added t...

Embodiment 2

[0050] The mensuration of the growth curve of embodiment 2 yeast A5

[0051] 1. Inoculate yeast A5 into LB medium and liquid nutrient medium containing sulfamethazine / copper compound pollution respectively, place it at 25 ℃, 150 r / min shaker constant temperature culture, and respectively in 0.5, 1, 1.5 , 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 60, 72, 96, 120, 144, 168, 192, 216 h sampling, determination of culture medium OD 600 , zeroed with sterilized LB liquid medium.

[0052] The LB medium is: NaCl 5g / L, yeast extract 10g / L, trypsin 10g / L, water 1000mL, pH 7.0-7.2.

[0053] The liquid LB medium containing sulfamethazine / copper composite pollution is as follows: add sulfamethazine and heavy metal copper to the above-mentioned LB medium so that the concentration is 100 mg / L.

[0054] 2. The result is as follows Figure 4 As shown, the yeast A5 can also grow normally in the culture system containing sulfamethazine / copper compound pollution.

Embodiment 3

[0055] Example 3 Degradation / adsorption experiment of yeast A5 on sulfamethazine / copper composite pollution

[0056] 1. Experimental method

[0057] (1) Preparation of bacterial suspension:

[0058] Scrape 1~2 loops of bacteria A5 from the seed preservation slope, insert them into sterilized LB medium, wrap them up and place them in a constant temperature shaker incubator (25 ℃, 150 r / min), and expand the culture for 24~36 h. Take the culture solution of activated bacteria A5, centrifuge (6000 r / min, 25°C), collect the bacteria, wash twice with sterile water (6000 r / min, 25°C), and then resuspend with sterile water to prepare A certain concentration of bacterial suspension.

[0059] (2) Degradation / adsorption of sulfamethazine / copper composite pollution by bacteria A5:

[0060] Add a certain amount of sulfamethazine as the only carbon source to the inorganic salt medium that has been sterilized by high pressure, so that the concentration is 1 mg / L, and add a certain amount...

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Abstract

The invention discloses degradation / adsorption bacteria for sulfonamide antibiotics / heavy metal composite pollution and an application thereof, wherein the bacteria is Sakaguchia cladiensis A5 and ispreserved in Guangdong Center of Microbial Culture Collection at Jan. 26th, 2018, and is assigned the accession number of GDMCC No.60319. The bacterial strain has great degradation effect on sulfonamide antibiotics such as sulfamerazine, sulfamethoxazole and the like, and has adsorption effect on heavy metals, so that the bacterial strain can be used for treatment on sulfonamide antibiotics / heavymetal composite pollution to application objects comprising wastewater, waste residue, water environment, soil and the like, which contain the sulfonamide antibiotics / heavy metal composite pollutants.The bacterial strain can reduce damage on ecological environmental safety due to the antibiotics and heavy metal, is energy-saving and environment-friendly, and has extensive application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant treatment. More specifically, it relates to a sulfonamide antibiotic / heavy metal compound pollution degrading / adsorbing bacterium and application thereof. Background technique [0002] For a long time, antibiotics have been widely used in human medicine and animal disease treatment. Different doses of antibiotics are added according to different antibiotic types and uses to prevent diseases and promote animal growth. Due to the massive consumption, excretion and discarding of antibiotics, antibiotics have been commonly detected in different environmental media. Studies have found that after organisms ingest a large amount of antibiotics, in addition to being partially metabolized by the body, 40% to 90% are excreted in the form of original drugs or primary metabolites with feces and urine, and finally enter the soil through fertilization and other methods. The environment or ente...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F3/34B09C1/10C02F103/34C02F101/20C12R1/645
CPCB09C1/105C02F3/34C02F3/347C02F2101/20C02F2103/343C12N1/16C12N1/145C12R2001/645
Inventor 陈烁娜檀笑曾洁仪纪梦钿曾苏杭解启来
Owner SOUTH CHINA AGRI UNIV
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