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Method for separating and extracting epsilon-polylysine

A technology for polylysine and polylysine feed liquid, which is applied in the field of extraction and separation, can solve the problems of not eliminating the ion exchange process, no substantial progress, and large consumption of acid and alkali, and achieves elimination of acid and alkali consumption, The effect of easy industrial scale-up and short operation time

Active Publication Date: 2018-09-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, compared with the progress made in the fermentation level, the ε-PL post-extraction technology has not made substantial progress, mainly because the ion exchange process has not been eliminated.
[0010] As we all know, although ion exchange technology has the advantages of high product recovery rate, strong impurity removal ability and mature operation, its defects of large acid and alkali consumption and large amount of waste water in the activation and regeneration process are still unavoidable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1) Bacteria removal from the fermentation broth: after the fermentation broth is diluted, use a centrifuge to centrifuge the fermentation dilution to obtain the fermentation broth;

[0055] 2) Adjustment of pH value and preparation of inorganic salt solution: Dissolve 10 g of NaCl (10%) in 90 mL of ε-PL fermentation broth, and use 1.0 M NaOH to adjust the pH of the mixed solution to 4.0;

[0056] 3) Construction of the two-phase aqueous system: Add 10ml of ethanol (10%) to the ε-PL fermentation broth containing NaCl at pH 4.0 above, shake and mix well, then let stand at room temperature for 1.0h, and then reuse The liquid funnel removes the lower phase and collects the upper phase;

[0057] 4) Separation and extraction of ε-PL from the upper phase: the upper phase was subjected to vacuum distillation and concentration at 40° C. to recover ethanol, and the ε-PL concentrated solution was collected for vacuum drying to obtain ε-PL samples.

[0058] In this example, the re...

Embodiment 2

[0060] 1) Bacterial removal of the fermentation broth: after the fermentation broth is filtered through a plate frame, the fermentation clear liquid is obtained;

[0061] 2) pH adjustment and preparation of inorganic salt solution: 30g K 2 HPO 4 (30%) was dissolved in 90mLε-PL fermentation liquid, and the pH of the mixed solution was adjusted to 11.0 by using 1.0M NaOH;

[0062] 3) Construction of a two-phase aqueous system: to the above pH 11.0, containing K 2 HPO 4 Add 30ml of acetone (30%) to the ε-PL fermented supernatant liquid, fully oscillate and mix, and let it stand at room temperature for 3.0h, then remove the lower phase with a separatory funnel, and collect the upper phase;

[0063] 4) Separation and extraction of ε-PL from the upper phase: The upper phase was subjected to vacuum distillation and concentration at 60° C. to recover ethanol, and the ε-PL concentrated solution was collected for vacuum drying to obtain ε-PL samples.

[0064] In this example, the re...

Embodiment 3

[0066] 1) Bacteria removal from the fermentation broth: after the fermentation broth is diluted, use a centrifuge to centrifuge the fermentation dilution to obtain the fermentation broth;

[0067] 2) pH adjustment and preparation of inorganic salt solution: 200g (NH 4 ) 2 SO 4 (20%) was dissolved in 900mLε-PL fermentation broth, and 1.0M NaOH was used to adjust the pH of the mixed solution to 9.0;

[0068] 3) Construction of a two-phase aqueous system: to the above pH 9.0, containing (NH 4 ) 2 SO 4 Add 200ml of ethanol (20%) to the ε-PL fermentation clear liquid, shake and mix well, let stand at room temperature for 1.5h, remove the lower phase by centrifugation, and collect the upper phase;

[0069] 4) Separation and extraction of ε-PL in the upper phase: Add 900 mL of 20% (NH 4 ) 2 SO 4 Solution, shake and mix well, let stand at room temperature for 1.0h, centrifuge to remove the lower phase, and collect the upper phase;

[0070] 5) Separating and extracting ε-PL fr...

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Abstract

The invention discloses a method for separating and extracting epsilon-polylysine, and belongs to the technical field of extraction and separation. By utilizing an organic solvent / inorganic salt aqueous two-phase system, the method for separating and extracting epsilon-polylysine is provided, and the method for separating and extracting epsilon-polylysine has the advantages that the generation ofwaste water can be reduced, the operation steps are simplified, the extraction cost is reduced, the extraction recycling rate is high, and the purity of the extracted product is high. By means of themethod, epsilon-polylysine is separated and extracted, the recycling rate can reach 98% or above, and compared with traditional ion exchange extraction methods, the method has obvious advantages.

Description

technical field [0001] The invention relates to a method for separating and extracting ε-polylysine, belonging to the technical field of extraction and separation. Background technique [0002] ε-polylysine (ε-PL) is a homotype amino acid polymer produced by extracellular secretion of microorganisms such as Streptomyces, filamentous fungi or Bacillus, which generally consists of 25-35 L-lysine monomers through It is formed by dehydration condensation of α-COOH and ε-NH2, and its molecular weight is usually 2500-4500Da. [0003] Due to the advantages of good water solubility, strong thermal stability, and broad antibacterial spectrum, ε-PL is currently mainly used as a biological food preservative and widely used in the food industry in Japan, South Korea, Europe, America and other countries and regions. In 2014, my country also officially approved the application of ε-PL and its hydrochloride in the food fields such as fruits and vegetables, rice and flour products, meat pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
CPCC08G69/10
Inventor 陈旭升毛忠贵马玉张建华张宏建王柯
Owner JIANGNAN UNIV
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