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Bioactive polypeptide APMISAASVH, as well as preparation method and application thereof

A technology of biologically active polypeptides and derivatives, applied in the field of proteins, can solve problems such as human body risks, and achieve the effects of reducing body aging, enhancing functions, and improving quality

Active Publication Date: 2018-09-28
海南熊猫乳品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • Bioactive polypeptide APMISAASVH, as well as preparation method and application thereof
  • Bioactive polypeptide APMISAASVH, as well as preparation method and application thereof
  • Bioactive polypeptide APMISAASVH, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The artificial synthesis of embodiment 1 active peptide APMISAASVH

[0037] 1. Synthesis of Bioactive Peptides

[0038] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0039] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0040] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0041] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0042] 5....

Embodiment 2

[0078] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0079] 1. Determination of in vitro antioxidant activity of bioactive peptide APMISAASVH by [DPPH·] method

[0080] 1. Experimental reagents and instruments:

[0081] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Biologically active polypeptide APMISAASVH.

[0082] Main instruments: Sunrise microplate reader, product of Tecan Company of Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; analytical balance, product of Meitelei-tolido Company.

[0083]2. Experimental method:

[0084] (1) 1mmol / L [DPPH·]methanol solution

[0085] Weigh 0.349 mg [DPPH·] with an analytical balance and dissolve it in 1 mL of methanol solution to prepare a 1 mmol / L [DPPH·] methanol solution, store it in the dark on tin foil, and use i...

Embodiment 3

[0120] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0121] 1. Acute Heat Stress Survival Experiment of Bioactive Polypeptide APMISAASVH

[0122] 1. Experimental reagents and instruments:

[0123] Reagents: Caenorhabditis elegans, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Escherichia coli strain E.coli OP50, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Agar powder, Sinopharm Chemical Reagent Co., Ltd.; Yeast powder, Sinopharm Chemical Reagent Co., Ltd. Company; the biologically active polypeptide APMISAASVH obtained in Example 1.

[0124] Instruments and equipment: Likang RO15 pure water system, Likang Biomedical Technology Co., Ltd.; G136T Zealway intelligent high-temperature sterilization pot, Xiamen Zhiwei Instrument Technology Co., Ltd.; THZ-32 desktop constant temperature oscillator, Shanghai Precision Experimental Equipment Co., Ltd. ; TDL-40B centrifuge, Shanghai...

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Abstract

The invention relates to the field of protein, in particular to bioactive polypeptide APMISAASVH, as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide APMISAASVH is Ala-Pro-Met-Ile-Ser-Ala-Ala-Ser-Val-His. In vitro antioxidant experiments and in vivo anti-aging experiments prove that the polypeptide APMISAASVH has good antioxidant biologicalactivity and anti-aging activity; on the one hand, the bioactive polypeptide APMISAASVH has good antioxidant activity and can eliminate free radicals in bodies and improve the life quality, and on the other hand, the bioactive polypeptide APMISAASVH can improve the vitality of in vivo peroxidase resistance system, and enhance the function of resisting exogenous stimulation of bodies, thereby reducing the probability of body aging, aging and sickness, and having great importance on the development of food, health products and medicines with an antioxidant function and an anti-aging function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide APMISAASVH and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Lactic acid bacteria cells also have some self-synthesized protein polypeptide fragments for bacterial growth and utilization. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has...

Claims

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Application Information

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IPC IPC(8): C07K14/335A61K38/16A61P37/02A61P39/06A23L33/195A23L33/18
CPCA23L33/18A23L33/195A23V2002/00A61K38/00A61P37/02A61P39/06C07K14/335A23V2200/324A23V2200/302
Inventor 张少辉林文珍洪志骏陈平陈承余林学海高兴明占东升
Owner 海南熊猫乳品有限公司
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