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A kind of sugar composition with promoting effect of wound healing and application thereof

A technology of wound healing and composition, applied in the field of sugar composition, which can solve the problem of limited biological activity

Active Publication Date: 2020-11-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biological activity of each polysaccharide or oligosaccharide is limited

Method used

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  • A kind of sugar composition with promoting effect of wound healing and application thereof
  • A kind of sugar composition with promoting effect of wound healing and application thereof
  • A kind of sugar composition with promoting effect of wound healing and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The carbohydrate composition of the present invention includes at least five of carboxymethyl chitosan, bletilla striata polysaccharide, fucoidan sulfate, heparan sulfate, chondroitin sulfate, hyaluronic acid oligosaccharide and guluronic acid oligosaccharide. The above-mentioned 7 kinds of carbohydrates can be prepared by the preparation method of the present invention, or commercially available products.

[0028] The preparation process of described carboxymethyl chitosan is as follows: dissolving chitosan in sodium hydroxide and isopropanol solution, then adding dropwise chloroacetic acid dissolved in isopropanol, then adding ethanol to terminate the reaction, filtering After washing and dialysis, freeze-dry to obtain carboxymethyl chitosan.

[0029] The preparation process of the bletilla striata polysaccharide is as follows: pulverize the bletilla striata, extract it with hot water, filter, dialyze, add ethanol to the dialyzed solution, freeze-dry the obtained prec...

Embodiment 2

[0038] Example 2: Effect experiment of carbohydrate compounds on HUVEC cell proliferation

[0039] Carbohydrates were used to determine the proliferation activity of human umbilical vein endothelial cells (HUVEC, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences) at a certain dose: after the cells were cultured, they were digested with 0.25% trypsin, and the cells were counted. cells according to 2×10 4 The number of cells / well was added to a 96-well plate (180 μL / well). After culturing for 6 hours, the culture solution was aspirated and replaced with a carbohydrate composition dissolved in the cell culture medium (180 μL / well). The same volume of cell culture medium As a control, culture for 24 hours, then add 20 μL / well of MTT solution (dissolved in PBS, 5 mg / mL) under the condition of avoiding light, continue to cultivate for 4 hours, then completely suck out the solution in the well, add 150 μL of DMSO, and shake at 37...

Embodiment 3

[0042] Example 3: Effect experiment of 12 kinds of carbohydrate compositions on HUVEC cell proliferation

[0043] Determination of HUVEC cell proliferation activity promoted by 12 kinds of sugar compositions: After the cells were cultured, they were digested with 0.25% trypsin, and the cells were counted, and the cells were counted according to 2×10 4 The number of cells / well was added to a 96-well plate (180 μL / well). After culturing for 6 hours, the culture solution was aspirated and replaced with a carbohydrate composition dissolved in the cell culture medium (180 μL / well). The same volume of cell culture medium As a control, culture for 24 hours, then add 20 μL / well of MTT solution (dissolved in PBS, 5 mg / mL) under the condition of avoiding light, continue to cultivate for 4 hours, then completely suck out the solution in the well, add 150 μL of DMSO, and shake at 37 ° C. Incubate in the bed for 10 minutes, then measure the absorbance A at 490 nm with a microplate reader, ...

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Abstract

The invention provides a carbohydrate composition with a wound healing promoting effect and application of the carbohydrate composition. The carbohydrate composition contains at least five types of carbohydrate including carboxymethyl chitosan, rhizoma bletillae polysaccharides, fucoidan from fucus vesiculosus, heparan sulfate, chondroitin sulfate, oligosaccharides of hyaluronan and gulose aldehyde acid oligosaccharides. The carbohydrate composition and the application have the advantages that VEGFA (vascular endothelial growth factor A), FGF2 (fibroblast growth factor 2) and VE-cadherin protein mRNA (messenger ribonucleic acid) expression can be obviously improved by the carbohydrate composition, VEGF (vascular endothelial growth factor) secretion and skin fibroblast (HSF) and human immortalized keratinocyte (HaCaT) proliferation can be obviously promoted by the carbohydrate composition, and the obvious wound healing promoting effect can be realized by the carbohydrate composition; raw materials required by the carbohydrate composition are easily available, composition methods for the carbohydrate composition are simple, the carbohydrate composition is low in cost, easy to industrialize and wide in application range, and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a sugar composition capable of promoting wound healing and an application thereof. Background technique [0002] At present, there are many studies on the use of sugar-based medical materials for wound healing, and there are many polysaccharides with related activities. For example, as a water-soluble chitosan derivative carboxymethyl chitosan, compared with chitosan, carboxymethyl Methyl chitosan has better antibacterial activity (Anitha A, et al.Carbohydrate Polymers, 2009,78(4):672-677), and it has been reported that carboxymethyl chitosan can be used at a concentration of 0.5mg / mL Left and right promote the proliferation of HUVECs (Jiang Z, et al. Carbohydrate Polymers, 2015, 129:1-8). Other studies have shown that carboxymethyl chitosan has a hemostatic effect (Janvikul W, et al. Journal of Applied Polymer Science, 2006, 102(1):445-551); promotes proliferati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/722A61K31/737A61K31/715A61K31/727A61K31/702A61P17/02
CPCA61K31/702A61K31/715A61K31/722A61K31/727A61K31/737A61P17/02A61K2300/00
Inventor 于广利丁琅赵小亮单鑫迪郝杰杰蔡超李国云
Owner OCEAN UNIV OF CHINA
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