Biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil and preparation and application thereof
A technology of biological bacterial fertilizer and soil improvement, applied in the direction of chemicals, applications, biocides, etc. for biological control, can solve the problem that the root rot of flue-cured tobacco cannot be fundamentally eliminated, and the pursuit of farmers' economic interests is difficult to achieve. Polluted water bodies and pesticide bioaccumulation and other problems, to achieve the effect of improving root rot resistance, inducing plant resistance resistance, good antagonistic and competitive effects
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Embodiment 1
[0046] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:
[0047] Biological fertilizer seed bacteria fermentation broth (volume percentage: Trichoderma harzianum 20%, Bacillus megaterium 15%, Bacillus subtilis 20%, Bacillus pumilus 15%, Trichoderma viride 15%, Lactobacillus acidophilus 15%), peanut bran;
[0048] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:
[0049] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contami...
Embodiment 2
[0060] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:
[0061] Biological fertilizer seed bacteria fermentation broth (volume percentage: Trichoderma harzianum 15%, Bacillus megaterium 20%, Bacillus subtilis 20%, Bacillus pumilus 20%, Trichoderma viride 15%, Lactobacillus acidophilus 10%), peanut bran;
[0062] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:
[0063] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contami...
Embodiment 3
[0074] A biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil, each component in the biological bacterial fertilizer is made according to the following ratio:
[0075] Biological fertilizer seed bacteria fermentation liquid (volume percentage: Trichoderma harzianum 10%, Bacillus megaterium 20%, Bacillus subtilis 20%, Bacillus pumilus 20%, Trichoderma viride 15%, Lactobacillus acidophilus 15%), peanut bran;
[0076] The preparation method of the biological bacterial fertilizer for preventing flue-cured tobacco root rot and improving soil comprises the following steps:
[0077] A. Insert Trichoderma harzianum in a dormant state stored in sand tubes and freeze-drying tubes into Chapei solid medium, and Bacillus megaterium, Bacillus subtilis and Bacillus pumilus into beef extract peptone solid medium , Trichoderma viride and lactic acid bacteria were inserted into the MRS solid medium, and each strain used a separate medium to avoid contamin...
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