Multi-stage fermentation production method for highly yielding spore clostridium butyricum

Abstract

The invention relates to the technical field of a clostridium butyricum fermentation process, in particular to a multi-stage fermentation production method for highly yielding spore clostridium butyricum. The method comprises the steps of seed liquid preparation, primary oxygen fermentation culture, secondary fermentation culture and adsorption drying, wherein glucose is supplemented in the secondary fermentation process; the temperature and the pH value are controlled in the whole process; the low-speed stirring fermentation culture is performed in the whole process; the dissolved oxygen riskis eliminated. The spore formation rate is a hundred percent; the microbial content of the clostridium butyricum of the bacterium liquid is high; the spore loss rate of the clostridium butyricum is low; the multi-stage fermentation is more favorable for the high-temperature resistance of the spores; the microbial content in the bacterium liquid is 2.5 to 3 billion / ml; the heat loss of the bacterium powder in the adsorption drying process is improved; the live spore number of clostridium butyricum in the bacterium powder is improved by 1 to 1.5 times; the microbial content in the bacterium powder reaches 0.4 to 0.5 billion / g; the live spore number and the butyrate content in the bacterium powder are improved; the product quality and the competitive power are improved; the obvious economicbenefits are realized.

Description

technical field [0001] The invention relates to the technical field of Clostridium butyricum fermentation technology, in particular to a multistage fermentation production method for high-yielding spore-forming Clostridium butyricum. Background technique [0002] Clostridium butyricum, Clostridium butyricum, also known as butyric acid bacteria, is a species in the genus Clostridium, which exists in cheese, natural yogurt, human and animal intestines and feces. Clostridium butyricum is a straight or curved Gram-positive anaerobic endophytic bacillus. It is a probiotic strain that has been recognized by research. It is widely used in intestinal medicine and health food in clinical medicine and animal husbandry. , feed additives and microbial fertilizers, etc., is an ideal microecological preparation with broad development and application prospects. Clostridium butyricum is resistant to gastric acid and bile salts when it passes through the intestinal tract of animals, which e...

AI Technical Summary

Problems solved by technology

Existing clostridium butyricum anaerobic fermentation process has the following defects: (1) anaerobic conditions are not sufficient, and a small amount of oxygen remains easily; (2) the fermentation process lacks feeding technology; (3) the number of fermented bacteria obtained is 10- 15×10 8 Bacteria liquid of cfu / ml; (4) The culture is based on the spore formation rate maturity index, rather than the number of viable spores and the butyrate content in the bacterial powder that can better reflect the product quality as the maturity index, which affects the quality of the product

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