In vitro identification method of coffee germplasm rust resistance
An identification method and resistance technology, which is applied in the field of in vitro identification of coffee germplasm rust resistance, can solve problems such as none, reduce experimental errors, facilitate popularization, and improve work efficiency
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Embodiment 1
[0031] A preparation method for rust fungus urediospores for in vitro identification of coffee rust resistance, comprising the steps of:
[0032] 1) Preparation of the inoculation culture box: Soak the sponge with 72% alcohol for 4 minutes, wherein the sponge is 1 cm thick, 15 cm long, and 10 cm wide, then air-dried, spread on the bottom of the plastic culture box, and soak the sponge with sterile water;
[0033] 2) From the field in the main coffee producing area of Yingjiang County, Yunnan Province, collect coffee leaves with typical coffee rust spots, gently scrape off the uredospores of the rust fungus with a scalpel, and apply the uredospores of the rust fungus on the affected areas. The diseased variety Matari has just unfolded, and the back of the young and soft leaves is lightly brushed with a brush to evenly distribute the uredia spores of the rust fungus, and sprayed with water mist until a water film is formed, then the back of the inoculated leaves faces upwards, ...
Embodiment 2
[0045] A kind of rust fungus urediospores that is used for in vitro identification of coffee rust resistance, the preparation method of described rust fungus uredospores is as follows:
[0046] 1) Preparation of the inoculation culture box: Soak the sponge with 75% alcohol for 5 minutes, wherein the sponge is 1 cm thick, 15 cm long, and 10 cm wide, then air-dried, spread on the bottom of the plastic culture box, and soak the sponge with sterile water;
[0047] 2) From the field in the main coffee producing area of Yingjiang County, Yunnan Province, collect coffee leaves with typical coffee rust spots, gently scrape off the uredospores with a scalpel, and apply the uredospores of the rust fungus to the susceptible varieties Caturra has just unfolded, and the back of the young and soft leaves is lightly brushed with a brush to evenly distribute the uredia spores of the rust fungus, and sprayed with water mist until a water film is formed, then the back of the inoculated leaves ...
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