Method for steam explosion cleaning refining polygeneration by using shrimp and crab shells
A technology for polygeneration of shrimp and crab shells, applied in chemical instruments and methods, carboxylate preparation, carboxylate preparation, etc., can solve the problem of difficult high-value utilization of a large number of components, serious environmental pollution to avoid the use of inorganic acids and bases, the process is easy to control, and the effect of improving economic value
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Embodiment 1
[0014] (1) Organic acid decalcification: Add 100 g of fresh or dried shrimp and crab shell raw materials, add 1 M lactic acid, soak at room temperature, solid-liquid ratio (w / w) is 1:10, soak for a certain time 12 After h, solid-liquid separation is carried out to obtain decalcified shrimp and crab shells, which are washed with water to pH 6.56;
[0015] (2) Steam explosion treatment: The decalcified shrimp and crab shells washed to near neutrality are subjected to steam explosion treatment under a pressure of 0.8MPa for 15 minutes;
[0016] (3) Biological deproteinization: The shrimp and crab shell materials after steam explosion treatment are completely deproteinized into chitin with proteolytic enzymes, and the conditions for enzymatic deproteinization are: neutral protease dosage 25 U / g, solid-liquid Ratio (w / w) 1:10, enzymatic hydrolysis temperature is 45 o C, enzymatic hydrolysis time 2 h, pH 7; shrimp and crab shell raw materials were treated with organic acid decalcif...
Embodiment 2
[0018] (1) Organic acid decalcification: Add 100 g of fresh or dried shrimp and crab shell raw materials, add 1M citric acid solution, soak at room temperature, solid-liquid ratio (w / w) is 1:5, soak for a certain period of time After 24 h, solid-liquid separation was carried out to obtain decalcified shrimp and crab shells, which were washed with water to pH 5.98;
[0019] (2) Steam explosion treatment: The decalcified shrimp and crab shells washed to near neutrality were subjected to steam explosion treatment under a pressure of 1.2 MPa for 20 minutes;
[0020] (3) Biological deproteinization: the shrimp and crab shell materials after steam explosion treatment are completely deproteinized into chitin with proteolytic enzymes, and the conditions for enzymatic deproteinization are: papain dosage 40 U / g, solid-liquid ratio (w / w) 1:10, enzymatic hydrolysis temperature is 60 o C, Enzymolysis time 1 h, pH 5.5; Shrimp and crab shell raw materials were treated with organic acid deca...
Embodiment 3
[0022] (1) Organic acid decalcification: add fresh or dried raw shrimp and crab shells to 6 M gluconic acid solution and soak them at room temperature with a solid-to-liquid ratio (w / w) of 1:20 for a certain time of 6 h Finally, solid-liquid separation is carried out to obtain decalcified shrimp and crab shells, which are washed with water to a near neutral pH of 6.65;
[0023] (2) Steam explosion treatment: The decalcified shrimp and crab shells washed to near neutrality were subjected to steam explosion treatment under a pressure of 1.5 MPa for 30 minutes;
[0024] (3) Biological deproteinization: The shrimp and crab shell materials after steam explosion treatment are fermented with the strain Aspergillus niger capable of producing proteolytic enzymes, and the materials are completely deproteinized and converted into chitin. The fermentation conditions are: solid-liquid ratio (w / w ) is 1:8, the fermentation temperature is 37 o C, the fermentation time is 4 days, after the s...
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