Dihydrochalcone glucoside derivative and extraction method thereof

A technology of dihydrochalcone glycoside and dihydrochalcone aglycone is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., and can solve the problem of lack of extraction and separation of dihydrochalcone derivatives. The method, there is no composition report, no separation and purification and other problems, and the effects of eliminating the enzyme treatment step, large output and improving production efficiency are achieved.

Inactive Publication Date: 2018-08-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: due to the limitations of extraction raw materials, extraction methods, and separation techniques, there is currently a lack of convenient, economical, and effective methods for the extraction and separation of dihydrochalcone glycoside derivatives. limit the application of these compounds
For example, in the genus Malus, phloretin 2'-xyloglucoside, as a glycoside derivative of dihydrochalcone, was discovered very early, but there has been no report of a large number of isolation and extraction, and some dihydrochalcone Other glycosylated derivatives of hydrochalcone, such as rutinoside derivatives, xylogalactoside derivatives, and sambubiose derivatives, were only detected by liquid chromatography-mass spectrometry, and there was no precedent for separation and purification
Therefore, the application of the activity of these compounds is also limited. For example, the antioxidant activity and bacteriostatic activity of the glycoside derivatives of dihydrochalcone are considered to be the main biological activities of this type of compound, but there is no research on this type of compound. Composition reports for active applications

Method used

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  • Dihydrochalcone glucoside derivative and extraction method thereof
  • Dihydrochalcone glucoside derivative and extraction method thereof
  • Dihydrochalcone glucoside derivative and extraction method thereof

Examples

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Embodiment 1

[0051] The extraction method of dihydrochalcone glycoside derivatives provided in this example comprises the following steps:

[0052] (1) After the raw materials used for extraction are processed through freeze-drying, get 2kg and soak in the aqueous solution of acidified alcohol, for improving the extraction rate, methods such as heating, pressurization, stirring, ultrasound can be used, and the solid phase will be separated from the liquid phase after extraction. And the liquid phase part is recovered, and the liquid phase part can be processed by extraction, vacuum distillation, normal temperature distillation, freeze-drying, heat-drying, centrifugation and other methods to obtain a crude extract to facilitate subsequent operations.

[0053] (2) The crude extract is further separated by macroporous resin chromatography, polyamide column chromatography, gel column chromatography, and reverse-phase column chromatography, and is eluted with eluents of different polarity and ac...

Embodiment 2

[0061] The extraction method of dihydrochalcone glycoside derivatives provided in this example comprises the following steps:

[0062] (1) After drying the raw materials for extraction, soak 500g in acidified 80% ethanol, recover the liquid phase, concentrate to obtain the crude extract, further separate the crude extract, and collect the dihydrochalcone glycosides contained in For the components of the derivatives, the components containing the dihydrochalcone glycoside derivatives are further separated and purified by high-speed countercurrent chromatography and reverse phase chromatography, so as to obtain pure dihydrochalcone glycoside derivatives.

[0063] (2) Use NMR and LC-MC to determine the structure of the obtained dihydrochalcone glycoside derivatives, and use HPLC to check the purity. Finally, it is determined that the obtained compound is Phloretin 2'-rutinoside, about 60 mg. The structure is determined by nuclear magnetic resonance and mass spectrometry as follows...

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Abstract

The invention belongs to the technical field of natural product chemistry, and discloses a dihydrochalcone glucoside derivative, an extraction method, an antioxidant composition and an antibacterial composition. The invention provides the dihydrochalcone glucoside derivative with a general formula I. The compound is extracted from unprocessed plant tissue materials. The extraction process comprises the steps that extraction is conducted in the plant tissue materials with an alcohol aqueous solution, and crude extracts are obtained; the dihydrochalcone glucoside derivative is obtained from thecrude extracts. The dihydrochalcone glucoside derivative can be used for preparing the antioxidant and antibacterials compositions.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a dihydrochalcone glycoside derivative and an extraction method thereof. Background technique [0002] Dihydrochalcone compounds exist in plants such as Rosaceae, Rutaceae, and Lithiaceae in the form of various glycosylated derivatives. The dihydrochalcone derivatives containing two sugar glycosides are considered to have the effect of inhibiting the oxidation of lipids in the diet during digestion and absorption in the human intestine; at the same time, they can also promote some allergenic food sources Protein cross-linking, thereby reducing the allergenicity of these proteins; in addition, such dihydrochalcone derivatives also have the effect of regulating the human intestinal flora. [0003] To sum up, the problems existing in the prior art are: due to the limitations of extraction raw materials, extraction methods, and separation techniques, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/203A61P31/04A61P39/06
CPCA61P31/04A61P39/06C07H1/08C07H15/203
Inventor 李鹏民肖郑操
Owner NORTHWEST A & F UNIV
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