Antibiological inoculant for preventing and controlling tobacco rhizome type diseases and preparation method thereof

A technology of biocontrol fungi and rhizomes, which is applied in the field of biocontrol fungi and its preparation for the prevention and treatment of tobacco rhizome diseases. It can solve the problems of direct output loss, aggravated damage, unstable control effect, etc., and achieve the goal of promoting tobacco growth. , reduced use, good application prospects

Active Publication Date: 2018-08-10
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The three main rhizome diseases often occur together, aggravating the degree of damage. In many old diseased fields or dry tobacco fields in severely diseased areas, the phenomenon of whole field disease and no yield occurs every year, resulting in direct output value caused by the decline in tobacco leaf yield and quality. very serious loss
[0003] The main infection sources of the three kinds of tobacco rhizome diseases all come from the soil, especially the fields where the disease has occurred over the years, and there are great difficulties in prevention and control
At present, biological control methods can reduce the damage of bacterial wilt to a certain extent, but there are still limitations such as unstable control effects in practical applications

Method used

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  • Antibiological inoculant for preventing and controlling tobacco rhizome type diseases and preparation method thereof
  • Antibiological inoculant for preventing and controlling tobacco rhizome type diseases and preparation method thereof
  • Antibiological inoculant for preventing and controlling tobacco rhizome type diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 : Screening and Identification of Strains

[0036] (1) Screening of strains

[0037] The 2 strains of biocontrol bacteria contained in the composite microbial bacterial agent of the present invention (the original numbers of the strains are respectively 16029 and 16098) are all isolated and screened from the cassava root soil in the cassava main production area of ​​Hepu County, Beihai City, Guangxi Zhuang Autonomous Region , the above-mentioned strains have been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures in January 2018, referred to as CGMCC. The preservation numbers of strain 16029 and strain 16098 are CGMCC No.15165 and CGMCC No.15166, respectively.

[0038] (2) Classification and identification of bacterial strains

[0039] Through the analysis of colony morphology, 16S rDNA sequence analysis, Biolog bacterial identification system, specific amplification PCR of BCC flora, and detection o...

Embodiment 2

[0040] Example 2 : Preparation of biocontrol agents

[0041] The fermented liquid of the biocontrol agent of the present invention is obtained by mixed culture of bacterial strain 16029 and bacterial strain 16098 seed liquid, based on the following experimental screening results:

[0042] (1) Both strain 16029 and strain 16098 had the effect of dissolving phosphorus and potassium, but the effect of strain 16029 was better than that of strain 16098.

[0043] (2) Both strains can inhibit tobacco bacterial wilt and other important plant pathogenic bacteria, and have biocontrol potential, but the biocontrol effect of strain 16098 is better than that of 16029. The bacterial suspension and culture stock solution of the two strains had strong antibacterial effect, but the antibacterial effect of the sterile filtrate was not obvious. For the antibacterial effect screening of two strains of BCC bacteria, see figure 1 .

[0044] (3) Under the same culture conditions, the growth cur...

Embodiment 3

[0052] Example 3 : the plant growth-promoting characteristics of the biocontrol agent of the present invention

[0053] (1) Determination of Phosphorus and Potassium Dissolving Effect of Bacteria by Soil Culture Method

[0054] Mix the basic cultivation soil red loam and the seedling-raising substrate in equal volumes, add appropriate amount of calcium phosphate and potassium feldspar powder, mix evenly, bottle and sterilize, inoculate the bacterial agent fermentation liquid of the present invention, the inoculation amount is 3%, and the control receives the same amount without After 20 days, the available phosphorus and available potassium contents of each treatment were measured. Available phosphorus was determined by molybdenum antimony anti-colorimetric method, and available potassium was determined by flame photometry.

[0055] The experimental results showed that the available phosphorus and available potassium contents of the mixed matrix in the early stage of inocul...

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Abstract

The invention belongs to the technical field of microorganism application, and particularly relates to an antibiological inoculant for preventing and controlling tobacco rhizome type diseases and a preparation method thereof. The antibiological inoculant for preventing and controlling tobacco rhizome type diseases is characterized by comprising the major ingredients consisting of fermentation liquid obtained through mixed fermentation of strains 16029 and strains 16098. The antibiological inoculant can achieve the strong inhibition effects on three kinds of major pathogenic bacteria of rhizomediseases of tobacco bacterial wilt, tobacco black shank and black root rot diseases, and can be used as the biocontrol bacterial agent for the tobacco rhizome type diseases. The bacteria agent has good application prospects on the prevention and control of tobacco rhizome type diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a biocontrol bacterial agent for preventing and treating tobacco rhizome diseases and a preparation method thereof. Background technique [0002] Tobacco is an important economic crop for leaves, and it is the main source of income for farmers in tobacco-growing areas to get rid of poverty and become rich. However, tobacco growth is seriously affected by root and stem diseases such as bacterial wilt, black shank and root black rot. Tobacco root and stem diseases are in the The occurrence is particularly serious in tropical and subtropical smoking areas, and it is a devastating disease that threatens world tobacco production. The three main rhizome diseases often occur together, aggravating the degree of damage. In many old diseased fields or dry tobacco fields in severely diseased areas, the phenomenon of whole field disease and no yield occurs every...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20
Inventor 胡春锦曾泉史国英岑贞陆农泽梅叶雪莲
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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