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Chitosan-based antigen release rate-adjustable nano-vaccine, and preparation method and application thereof

A nano-vaccine and chitosan technology, applied in the field of biomedicine, can solve the problems of T cell immune tolerance exhaustion, achieve the effect of improving humoral and cellular immune responses, clear ingredients, and high encapsulation efficiency

Inactive Publication Date: 2018-08-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current vaccines are not yet capable of inducing an effective immune response against cancer, especially in the elderly
[0003] Biomaterials have been used to improve their safety and efficiency, such as emulsions, hydrogels, and microparticles, but these materials are prone to immune tolerance and exhaustion of T cells in the body

Method used

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  • Chitosan-based antigen release rate-adjustable nano-vaccine, and preparation method and application thereof
  • Chitosan-based antigen release rate-adjustable nano-vaccine, and preparation method and application thereof
  • Chitosan-based antigen release rate-adjustable nano-vaccine, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of nanoparticles using FNC technology

[0046]FNC technology is a technology for preparing drug-loaded nanoparticles through electrostatic interaction in aqueous phase (recorded in the inventor's previous patent application number PCT / US2017 / 014080), and the preparation process is mainly carried out in a multi-inlet vortex mixer. , has the characteristics of high throughput and strong controllability, the prepared nanoparticles have uniform distribution and small particle size, and the difference between batches is small. Since there is no organic solvent involved in the whole process, it is especially suitable for the formulation of biological macromolecules such as proteins and nucleic acids. The multi-inlet vortex mixer comprises a first part located in the upper part, a second part located in the middle part and a third part located in the lower part, the first part, the second part and the third part are cylinders with the same diameter; The ...

Embodiment 2

[0056] Example 2 Characterization of particle size, potential and morphology

[0057] The particle size, polydispersity index and surface potential of the particles were measured using a Malvern particle sizer. The morphology of the particles was characterized by transmission electron microscopy. like figure 1 shown.

Embodiment 3

[0058] Example 3 Presentation of antigens by dendritic cells

[0059] Take 6-8 week old mice, extract mouse bone marrow cells, and induce differentiation in vitro to make them immature dendritic cells.

[0060] Preparation Example 1, Preparation Example 2, Comparative Preparation Example 1, and Comparative Preparation Example 2 were incubated with DC cells, respectively, and 24 or 48 hours later, dendritic cells were collected.

[0061] Dendritic cells were incubated with antigen presenting reporter cells. The absorbance of the system was detected by a microplate reader. like image 3 shown.

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Abstract

The invention belongs to the technical field of medicines, and provides a chitosan-based antigen release rate-adjustable nano-vaccine, and a preparation method and an application thereof. Nano-particles are prepared by a rapid nano-complexation technology, and can efficiently co-encapsulate an antigen. The nano-vaccine uses a protein as the antigen; a ratio of chitosan to sodium triphosphate is changed at a specific pH value to obtain nano-vaccines having the same surface charge, nanometer particle size and drug loading amount, and the nano-vaccines have different antigen release rates; and the nano-vaccine with a fast antigen release rate promotes the presentation of the antigen in order to induce a strong immune response in the body and effectively inhibit tumor growth. The nano-vaccinehas the advantages of easiness in preparation, controllable components, and realization of large-batch continuous production.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, and more particularly, to a chitosan-based nano-vaccine capable of regulating the release rate of antigen and a preparation method and application thereof. Background technique [0002] Cancer has become a serious threat to human health, and vaccines have shown great promise in the field of cancer prevention and treatment. With the continuous in-depth study of immunology, the increasingly mature genetic engineering technology and the rapid development of synthetic technology, human beings have developed a variety of new vaccines such as recombinant DNA vaccines and short peptide vaccines. Although these emerging vaccines have many advantages, such as easy synthesis, purification, and strong antigen specificity, their weak immunogenicity has become their fatal disadvantage, resulting in their inability to induce a strong immune response, limiting their clinical application. use on. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K39/00A61K47/36A61K47/30A61P37/04A61P35/00
Inventor 杨成彪刘利新陈永明
Owner SUN YAT SEN UNIV
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