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A method for constructing a mouse model of renal inducible intrinsic macrophage infiltration

A technology of macrophages and mouse models, applied to other methods of inserting foreign genetic materials, interleukins, chemical instruments and methods, etc., can solve the single factor that cannot be used as a research model of renal intrinsic macrophages and cannot function in the kidney Analysis and other issues

Active Publication Date: 2021-08-20
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

[0004] 1) Since this mouse is not a kidney-specific IL34 knockout mouse, let alone a kidney-specific IL34 overexpression mouse, it involves the comprehensive effects of multiple organs and systems, and there is an interaction between each system, so it is impossible to intuitively understand the kidney. One-way analysis of the effect;
[0005] 2) This mouse is also involved in the comprehensive effects of other immune organs, immune cells and other macrophage subtypes, so it cannot be used as a research model for intrinsic macrophages in the kidney

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  • A method for constructing a mouse model of renal inducible intrinsic macrophage infiltration
  • A method for constructing a mouse model of renal inducible intrinsic macrophage infiltration
  • A method for constructing a mouse model of renal inducible intrinsic macrophage infiltration

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Embodiment Construction

[0033] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0034] The construction process of the mouse model is as follows: figure 1 shown, including the following steps:

[0035] S1: Construction of LSL-IL34 mice with the IL34 conditional expression cassette;

[0036] S2: Constructing a KSP-iCreERT2 mouse with an active Cre recombinase expression cassette driven by the renal tubular tissue-specific promoter under induction conditions;

[0037] S3: The LSL-IL34 mouse was crossed with the KSP-iCreERT2 mouse, and the offspring mice obtained were the kidney-induced intrinsic macrophage infiltration mouse model.

[0038] 1. Construction of targeting vector

[0039] The schematic diagram of the first targeting carrier is as follows: figure 2 Shown, from 5' to 3' are CAG pr...

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Abstract

The invention provides a method for constructing a mouse model of renal inducible intrinsic macrophage infiltration, which includes the step of specifically expressing IL34 gene in mouse renal tubular cells under the induced condition. By constructing inducible mice with high expression of IL34 in renal tubules, a kidney mouse model characterized by intrinsic macrophage infiltration was successfully constructed, which can effectively avoid non-selective gene overexpression and non-selective cell infiltration, and reduce the impact on The influence of important extrarenal organs and the overall state of the body can also effectively improve the specificity of infiltration of intrinsic macrophages in the kidney. In addition, through this model, combined with bioinformatics analysis, the function of intrinsic macrophages of the kidney can be further explored, providing a reliable path for finding more specific biological markers of intrinsic macrophages, and providing a basis for targeting renal intrinsic macrophages. Drug screening in cells provides a reliable model for in vivo research.

Description

technical field [0001] The invention relates to the field of mouse disease models, in particular to a method for constructing a kidney inducible intrinsic macrophage infiltration mouse model. Background technique [0002] Macrophages are highly heterogeneous. During the resting period of the kidney, they maintain tissue homeostasis and tissue repair; in pathological conditions, they promote renal fibrosis. Most previous studies simply divided macrophages into M1 (inflammatory macrophages) and M2 (reparative macrophages), ignoring the phenotype, function, connotation and environmental response of macrophages. This complexity, while ignoring the phenomenon of M1 / M2 functional crossover, has led to ambiguity about the role of macrophages in kidney disease. Therefore, more and more researchers now advocate that macrophages in tissues can be divided into peripheral macrophages and intrinsic macrophages (Tissue-resident macrophages) according to their origin. Peripheral macropha...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/90C12N9/22C12N15/66A01K67/027
CPCA01K67/0275A01K2227/105A01K2267/03C07K14/54C12N9/22C12N15/66C12N15/907
Inventor 曾锐廖文慧
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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