Hydrogel 3D printing material and preparation method thereof

A 3D printing and hydrogel technology, applied in the direction of additive processing, etc., can solve the problems of sol shaping difficulties, toxic cells, and easily damaged structures, and achieve non-toxic cells and tissues, good biocompatibility, and promote The effect of cell growth

Active Publication Date: 2018-08-03
天使召唤生物科技(重庆)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Texas Tech University and Texas A&M University introduced calcium ions by immersing 3D printed alginate structures in calcium chloride solution, and the two formed cross-links, making their tensile strength close to that of human natural cartilage, but This fully ionically cross-linked hydrogel is often brittle, and its structure is easily destroyed during subsequent use, and when it contacts human tissue fluid, it will undergo Ca2+-Na+ ion exchange and dissolve, and the structure cannot be maintained.
CN 103977453 A discloses a 3D bioprinting hydrogel material, which must be added with a photopolymerization initiator or / and a crosslinking agent to regulate the degree of crosslinking of the network structure of the hydrogel and improve the strength of the material, but the printed The sol is difficult to shape, difficult to maintain size, poor biocompatibility, and toxic to cells
CN103705982A discloses a preparation method of chitosan / hyaluronic acid / gelatin cross-linked composite porous scaffold and CN104327311A discloses a hyaluronic acid composite cross-linked hydrogel, both of which improve the mechanical properties of hyaluronic acid and prevent being Hyaluronidase enzymatic hydrolysis, adding a cross-linking agent in the gelation process for chemical group cross-linking, poor biocompatibility, low printing accuracy, and difficult to control the release of biomacromolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation method of hydrogel 3D printing material

[0017] (1) Preparation of gel A: at room temperature, add 12 parts of amino hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 8 parts of cellulose and stir thoroughly, and finally add 5 parts of cationic chitin and stir to mix well , then vortex the resulting mixture for 30 seconds, and place it in a water bath at 37°C for 2 minutes to obtain gel A;

[0018] (2) Preparation of gel B: Stir and mix 15 parts of alkaline gelatin and 35 parts of alginic acid at room temperature, then vortex the resulting mixture for 30 seconds, and place it in a water bath at 37°C for 2 minutes. That is B gel.

Embodiment 2

[0019] Embodiment 2: the preparation method of hydrogel 3D printing material

[0020] (1) Preparation of gel A: at room temperature, add 10 parts of aminated hyaluronic acid to 27 parts of water and stir to fully dissolve, then drop in 10 parts of cellulose and stir well, and finally add 3 parts of cationic chitin and stir to mix well , then vortex the resulting mixture for 50 seconds, and place it in a 35°C water bath for 3 minutes to obtain A gel;

[0021] (2) Preparation of gel B: Stir and mix 18 parts of alkaline gelatin and 32 parts of alginic acid at room temperature, then vortex the resulting mixture for 50 seconds, and place it in a water bath at 35°C for 3 minutes. That is B gel.

Embodiment 3

[0022] Embodiment 3: the preparation method of hydrogel 3D printing material

[0023] (1) Preparation of gel A: at room temperature, add 13 parts of amino hyaluronic acid to 25 parts of water and stir to fully dissolve, then drop in 6 parts of cellulose and stir thoroughly, and finally add 6 parts of cationic chitin and stir to mix well , then vortex the obtained mixture for 40 seconds, and place it in a water bath at 37°C for 2 minutes to obtain gel A;

[0024] (2) Preparation of gel B: Stir and mix 18 parts of alkaline gelatin and 32 parts of alginic acid at room temperature, then vortex the resulting mixture for 40 seconds, and place it in a water bath at 37°C for 2 minutes. That is B gel.

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Abstract

The invention provides a hydrogel 3D printing material and a preparation method thereof. The hydrogel 3D printing material is composed of a gel A and a gel B, wherein the gel A is prepared from, by weight, 8 to 15 parts of aminated hyaluronic acid, 5 to 12 parts of cellulose, 2 to 8 parts of cationic chitin and 20 to 30 parts of water; and the gel B is prepared from, by weight, 10 to 20 parts of alkaline gelatin and 30 to 40 parts of alginic acid. The invention also provides a preparation method for the hydrogel 3D printing material. The hydrogel 3D printing material of the invention has the advantages of good biocompatibility, good affinity to cells, no toxicity to cells and tissue, adjustability in shape and gap, controllability by any program and source code, safety, high efficiency, controllability, good rheological properties, good shear thinning characteristics and good flexibility; and all the raw materials used in the invention are clinically usable under the approval by the USFDA, and have good biodegradability, no harm to human beings and low cost.

Description

technical field [0001] The invention relates to a 3D printing material and a preparation method thereof, in particular to a hydrogel 3D printing material and a preparation method thereof. Background technique [0002] Three-dimensional printing is an additive manufacturing technology in industrial applications. Due to its high precision and strong manipulability, it is now developing rapidly in the fields of medical clinics and scientific research. Because of its three-dimensional network structure, hydrogel can simulate natural tissues, and as biopaper in three-dimensional bioprinting, it can meet the morphological requirements and support cell proliferation well, and can be used as a scaffold for cell growth, which has attracted widespread attention. In recent years, by controlling parameters such as hydrogel shape, porosity, surface morphology, and size, hydrogels have made a series of progress in the field of 3D bioprinting, but there are still many difficulties in actua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/04C08L89/00C08L5/08C08L1/02C08J3/075B33Y70/00
CPCB33Y70/00C08J3/075C08J2305/04C08J2401/02C08J2405/08C08J2489/00
Inventor 杨陈
Owner 天使召唤生物科技(重庆)有限公司
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