Method for realizing uniform liquid separation on hydrophobic polymer trace porous carrier

Abstract

The invention discloses a method for realizing uniform liquid separation on a hydrophobic polymer trace porous carrier. The hydrophilic modification method of the hydrophobic polymer trace porous carrier comprises the following steps: 1) cleaning a hydrophobic polymer trace porous carrier injection molded part; 2) placing the cleaned injection molded part into a generation cavity of a radio frequency plasma generator for surface modification treatment; 3) putting the surface-modified injection molded part into a hydrophilic modification enhancing liquid for soaking treatment; 4) taking out thesoaked injection molded part, draining off, and drying to obtain the hydrophilic trace porous carrier injection molded part. The obtained injection molded part has relatively high hydrophilicity, sothat the hydrophilic culture medium which is subpackaged or borne on the surface of the injection molded part can be rapidly and uniformly dispersed in each micro-hole under the small sub-packaging amount. A trace biochemical identification strip with the liquid separation function is treated by using the technology, after the trace biochemical identification strip is placed for 360 days, even liquid separation can still be carried out, and correct identification results can be guaranteed.

Description

technical field [0001] The invention relates to a method for realizing uniform liquid separation on a hydrophobic macromolecule microporous carrier, belonging to the field of biological reagents. Background technique [0002] Microbial biochemical identification is a comprehensive evaluation of the results of each biochemical reaction after the bacterial suspension to be identified is inoculated into a variety of biochemical reaction media and cultured. The traditional biochemical medium is divided into test tubes or vials, with a large volume of 2-10 mL, which is not conducive to storage, transportation and use. In order to improve this problem, trace biochemical identification strips (such as API, MID, etc.) products have appeared in the domestic and foreign markets at present. Each reaction well requires the user to drop the bacterial suspension separately, which still has the disadvantage of being inconvenient to use. The hydrophobic polymer biological reagent carrier ...

AI Technical Summary

Problems solved by technology

However, the injection molded parts purchased on the market are all hydrophobic or have only undergone preliminary modification treatment, and the hydrophilicity has a short time limit

[0003] CN107099455A discloses a visual microbial biochemical identification kit and its identification method, wherein in the identification kit, the fluidity of the aqueous solution is used to complete the distribution of the sample liquid, and it is proved by experiments that the hydrophobicity or the hydrophilicity after preliminary modification are not enough Strong injection molded parts only use the fluidity of the aqueous solution to complete the distribution of a small amount of sample liquid (100-105μL), and the liquid separation will be very uneven, which will lead to inaccurate biochemical reaction results. If the problem of uneven liquid separation is not solved , the hydrophobic polymer biological reagent carrier with liquid separation function cannot realize the application value

If plasma technology is used alone for hydrophilic treatment, there is a time-lapse effect. Within a few hours to one day after treatment, the hydrophilic surface is prone to rearrangement and returns to the hydrophobic surface, which greatly reduces the treatment effect and makes it difficult to achieve the separation of micropores. Liquid uniformity

[0005] Due to the reason of lubricating and protecting the mold in the conventional plastic injection molding process, special lubricating oil must be introduced into the mold, so that the injection molded parts will inevitably be stained with lubricating oil components, and the lubricating oil will form a typical lipophilic phase on the surface of the injection molded parts, which will not It is conducive to the rapid dispersion of hydrophilic medium, which will affect the accuracy of biochemical reactions, so cleaning lubricating oil is a necessary step before hydrophilic treatment

The conventional method to remove lubricating oil is mainly to clean with surfactant and deionized water, but for injection molded parts with liquid separation function, the complex and irregular structure brings great difficulty to cleaning, and the residual surfactant acts as a secondary Pollutants will affect the normal growth of organisms, and residual surfactants as secondary pollutants will affect the normal growth of organisms and cause abnormal results; at the same time, the drying process after conventional cleaning is complicated and wastes energy, which is harmful to the surface of transparent injection molded parts ( Especially the observation window structure) cause scratches and affect the performance

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