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Application of E3 ubiquitin ligase RNF14 in preparation of drug for treating fatty liver and related diseases

A technology of ubiquitin ligase and fatty liver, which is applied in the function and application field of genes, and achieves the effect of high safety

Inactive Publication Date: 2018-07-31
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the function and application of RNF14 in fatty liver have not been reported yet.

Method used

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  • Application of E3 ubiquitin ligase RNF14 in preparation of drug for treating fatty liver and related diseases
  • Application of E3 ubiquitin ligase RNF14 in preparation of drug for treating fatty liver and related diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0091] [Example 1] Effect of RNF14 Gene Overexpression on Fat Accumulation in Hepatocytes

[0092] Construct a lentiviral expression vector that overexpresses RNF14, transfect HEK-293T cells, package the lentivirus, infect L02 cells to construct a stable cell line that overexpresses RNF14, and overexpress the empty vector as a control (Con), and detect the stable cell line by WB Whether or not to express RNF14; L02 cells with successful expression were plated, and after the cells adhered to the wall, palmitate (PA, final concentration 0.2mM) and oleic acid (OA, final concentration 0.4mM) were stimulated, and a drug-dosing control group was set at the same time (adding the same After 12 hours, the cell samples of each group were collected and stained with Oil Red O.

[0093] WB test results such as figure 1 As shown, the protein expression level of RNF14 in the L02 cell line infected with RNF14-overexpressing lentivirus was significantly higher than that of the empty control g...

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Abstract

The invention discloses application of E3 ubiquitin ligase RNF14 in preparation of a drug for treating fatty liver and related diseases, and belongs to a new use of the RNF14. In the present invention, a L02 cell line overexpressed by human normal liver cell line L02 and the RNF14 is used as an experimental object, and the gene function of the RNF14 is studied by a fatty liver cell model co-induced by palmitic acid (PA) and oleic acid (OA). The study finds that RNF14 overexpression can significantly improve lipid accumulation in liver cells, indicating that the RNF14 can protect the liver cells from fatty degeneration, and the RNF14 plays a protective role in liver lipid metabolic diseases. Since the RNF14 is an endogenous protein kin the body, the RNF14 is highly safe as the drug.

Description

technical field [0001] The invention belongs to the field of gene function and application, and relates to the function and application of E3 ubiquitin ligase RNF14 in the treatment of fatty liver, and the use of RNF14 as a target gene in the development of drugs for preventing, alleviating and / or treating fatty liver and related diseases Applications. Background technique [0002] Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease in the world, with an incidence rate of 15% to 30% in Western countries, 27% in Hong Kong, China, and 27% in Southeast Asia, South Korea, Japan and Taiwan, China. 15% to 45%, and 20% in China[1]. Nonalcoholic fatty liver is divided into two categories, one is the milder nonalcoholic fatty liver (NAFL), and the other is the more severe nonalcoholic steatohepatitis (NASH). The continued development of non-alcoholic fatty liver can lead to cirrhosis and liver cancer, which seriously threatens human health [2]. [0003] The l...

Claims

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Application Information

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IPC IPC(8): A61K38/43A61K48/00A61P1/16A61P5/50A61P3/10A61P3/00A61P3/04A61P3/06A61P31/14A61P31/20A61P35/00
CPCA61K38/43A61K48/005C12Y603/02019
Inventor 李红良
Owner WUHAN UNIV
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