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Method for reducing endotoxin amount in bacterial cellulose shaping material

A bacterial cellulose and endotoxin technology, applied in medical science, surgery, etc., can solve the problems of reducing the mechanical properties of materials and long cycle times

Inactive Publication Date: 2018-07-27
涂青山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This can indeed reduce the content of endotoxin, but the cycle is very long (up to one month), and repeated mechanical extrusion greatly reduces the mechanical properties of the material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The first step: soak the gynecological pelvic floor patch of bacterial cellulose with a porosity of 30-60% and a pore diameter of 1-2mm in a NaOH solution with a temperature of 90°C and a molar concentration of 0.1 mol / L for 60 hours, and stir to make it Mix evenly, stirring speed is 100r / min, the mass ratio of the gynecological pelvic floor patch quality of bacterial cellulose and NaOH solution is 1: 90;

[0012] The second step: put the gynecological pelvic floor patch of bacterial cellulose treated with NaOH into pure water to dialyze at 40°C and 100r / min until the conductivity of the separated sodium hydroxide aqueous solution is lower than 1μS / cm to stop dialysis;

[0013] The third step: the gynecological pelvic floor patch of bacterial cellulose purified by the second step of dialysis is placed in a clean room and naturally dried until its water content is 18%;

[0014] Step 4: Seal and package the dried bacterial cellulose gynecological pelvic floor patch, irra...

Embodiment 2

[0017] Step 1: Soak the bacterial cellulose hernia patch with a porosity of 30-60% and a pore diameter of 1-2 mm in a KOH solution with a temperature of 50 ° C and a molar concentration of 10 mol / L for 72 hours, stir to make it evenly mixed, and stir The rotating speed is 70r / min, and the mass ratio of the hernia patch quality of bacterial cellulose to the KOH solution is 1: 20;

[0018] Step 2: Put the bacterial cellulose hernia patch treated with KOH into pure water for dialysis at a speed of 100 r / min at 20°C until the conductivity of the separated potassium hydroxide aqueous solution is lower than 1 μS / cm stop dialysis;

[0019] The third step: drying the bacterial cellulose hernia patch treated by the second step of dialysis purification at 80° C. in a drying oven until its water content is 30%;

[0020] Step 4: seal and package the dried bacterial cellulose ℃ patch, irradiate with gamma rays at a dose of 20 kGy for 60 seconds, and store.

[0021] The bacterial cellulos...

Embodiment 3

[0023] The first step: soak the gynecological pelvic floor patch of bacterial cellulose with a porosity of 30-60% and a pore diameter of 1-2mm in a LiOH solution with a temperature of 90°C and a molar concentration of 5 mol / L for 10 hours, and stir to make it Mix well, the stirring speed is 100r / min, and the mass ratio of the gynecological pelvic floor patch quality of bacterial cellulose to LiOH solution is 1:50;

[0024] The second step: put the gynecological pelvic floor patch of bacterial cellulose treated with LiOH into pure water for dialysis at 20°C and a speed of 100r / min until the conductivity of the separated sodium hydroxide aqueous solution is lower than 1μS / cm to stop dialysis;

[0025] The third step: the gynecological pelvic floor patch of bacterial cellulose purified by the second step of dialysis is placed in a clean room and naturally dried until its water content is 23%;

[0026] Step 4: Seal and package the dried bacterial cellulose gynecological pelvic fl...

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PUM

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Abstract

The invention provides a method for reducing endotoxin amount in a bacterial cellulose shaping material. The method comprises the following steps: separating and purifying a bacterial cellulose shaping material treated by alkali metal hydroxide by adopting a dialysis method through taking water as a solvent under a room-temperature condition; then drying and carrying out irradiation treatment by utilizing gamma rays with the dosage of 20 to 50kGy. Compared with an existing treatment method, the method provided by the invention has the greatest advantage that the amount of endotoxin in the bacterial cellulose shaping material can be effectively reduced to reach related standards of CFDA (China Food and Drug Administration); the requirements of clinical utilization are met; meanwhile, mechanical properties including strength of the shaping material and the like are basically not damaged.

Description

technical field [0001] The invention relates to a method for reducing harmful toxins in bacterial cellulose forming materials, in particular to a method for reducing the amount of endotoxin in bacterial cellulose forming materials. Background technique [0002] Gram-negative bacteria will release a large amount of endotoxin after death, and endotoxin can induce symptoms such as fever in the human body. Therefore, the State Food and Drug Administration (CFDA) has strict restrictions on the amount of endotoxin contained in medical devices . Bacterial cellulose forming material (BC for short) is prepared by fermentation of Acetobacter xylinum, but Acetobacter xylinum is a Gram-negative bacterium, so the endotoxin content of bacterial cellulose forming material is very large. The way to remove toxins is to cook with high-concentration strong sodium oxide at high temperature, or add other antibacterial substances (such as chitosan, etc.) to the cellulose produced by fermentation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J7/00C08J7/02C08L1/02A61L31/04A61L31/14
CPCA61L31/042A61L31/14C08J7/00C08J7/02C08J7/12C08J7/123C08J2301/02C08L1/02
Inventor 不公告发明人
Owner 涂青山
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