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Momordica grosvenori endophyte strain capable of producing cyclodextrin glycosyltransferase as well as screening method and application of momordica grosvenori endophyte strain

A glucose-based and cyclodextrin technology, applied in the field of microorganisms, can solve problems such as environmental pollution and waste of resources

Active Publication Date: 2018-07-13
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of this, it is necessary to develop a glucosyltransferase-producing endophytic strain of Luo Han Guo, which can not only solve the problem that the mogroside Ⅱ and saponin Ⅲ produced in the production of Luo Han Guo are discarded and pollute the environment, but also turn waste into treasure and avoid waste of resources

Method used

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  • Momordica grosvenori endophyte strain capable of producing cyclodextrin glycosyltransferase as well as screening method and application of momordica grosvenori endophyte strain
  • Momordica grosvenori endophyte strain capable of producing cyclodextrin glycosyltransferase as well as screening method and application of momordica grosvenori endophyte strain
  • Momordica grosvenori endophyte strain capable of producing cyclodextrin glycosyltransferase as well as screening method and application of momordica grosvenori endophyte strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Example 1: A Monk Fruit endophyte strain producing cyclodextrin glucosyltransferase

[0143] A strain of Luo Han Guo endophytic bacteria producing cyclodextrin glucosyltransferase, the name of the strain is Bacillus sp.ND-6 (Bacillus sp. CGMCC for short), the deposit number is CGMCC No.15227, and the deposit date is January 16, 2018.

Embodiment 2

[0144] Example 2: A screening method for the above-mentioned Monk Fruit endophytic strain producing cyclodextrin glucosyltransferase

[0145] A method for screening the above-mentioned endophytic strains of Luo Han Guo producing cyclodextrin glucosyltransferase, comprising the steps of:

[0146] Step 1: The method for isolating and purifying the endophytic strain of Luo Han Guo from the roots, stems and fruits of Luo Han Guo is:

[0147] Disinfection of Luo Han Guo materials: On the ultra-clean workbench, the cleaned roots, stems and fruits of Luo Han Guo are subjected to surface disinfection treatment by the following methods.

[0148] Roots: Soak in 75% (v / v) alcohol for 2 minutes, then soak in 3.0% NaClO solution for 5 minutes, then soak in 75% (v / v) alcohol for 30 seconds, and finally rinse with sterile water for 5 times. 5 minutes each time.

[0149] Stems: Soak in 75% (v / v) alcohol for 3 minutes, then soak in 3.0% NaClO solution for 8 minutes, then soak in 75% (v / v) al...

Embodiment 3

[0173] Implementation 3: Identification of the above-mentioned Monk Fruit endophytic strains producing cyclodextrin glucosyltransferase

[0174] Such as figure 1 , figure 2 As shown, the endophyte strain of Luo Han Guo producing cyclodextrin glucosyltransferase has the following characteristics:

[0175] (1) Individual morphology: the bacterium is rod-shaped, Gram staining is positive, and there are spores, and the spores are located in the middle of the bacterium.

[0176] (2) Colony traits on solid medium (refer to the above-mentioned slant medium for the formula) plate: the colony is round, wrinkled in the middle, smooth at the edge, and slightly yellow.

[0177] (3) The fermentation liquid in the liquid fermentation medium is turbid, with a bacterial film on the liquid surface and flocculent sediment at the bottom.

[0178] (4) The growth temperature is 30-45°C, the natural pH value; the enzyme production temperature is 35-45°C, the pH value is 8.0, aerobic; the enzyme...

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Abstract

The invention discloses a momordica grosvenori endophyte strain capable of producing cyclodextrin glycosyltransferase as well as a screening method and the application of the momordica grosvenori endophyte strain, and belongs to the technical field of microorganisms. The strain is called bacillus sp. ND-6 in the CGMCC, with a collection number of No.15227, on January 16, 2018. The invention further discloses a screening method of the momordica grosvenori endophyte strain capable of producing the cyclodextrin glycosyltransferase, a method for producing the cyclodextrin glycosyltransferase by using the momordica grosvenori endophyte strain capable of producing the cyclodextrin glycosyltransferase, and the application of the cyclodextrin glycosyltransferase. The momordica grosvenori endophytestrain disclosed by the invention can produce the cyclodextrin glycosyltransferase, and the cyclodextrin glycosyltransferase is high in enzyme activity (10,660 to 11,200 U / mL).

Description

technical field [0001] The invention relates to a Monk Fruit endophyte strain producing cyclodextrin glucosyltransferase, a screening method and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Luo Han Guo [Siraitia grosvenorii (Swingle) C. Jeffrey] is the fruit of the perennial rhizome vine Luo Han Guo of Cucurbitaceae Luo Han Guo. It is a unique plant in my country and grows in Guangxi, Guangdong, Jiangxi, Hunan and Guizhou. Yongfu County is the birthplace and main producing area of ​​Luo Han Guo. Luo Han Guo fruit is rich in a variety of essential amino acids, trace elements, and multivitamins. In addition, it contains active ingredients such as flavonoids, polysaccharides, sweet glycosides, and polyphenols. Modern pharmacological research has confirmed that Luo Han Guo has a variety of pharmacological effects such as antitussive and asthma, laxative, lowering blood sugar, anti-oxidation and improving immunity, etc., a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N9/10C12P19/56C12R1/07
CPCC12N1/02C12N9/1074C12P19/56C12Y204/01019C12N1/205C12R2001/07
Inventor 赵丰丽张昌志范彩琴龙楚媚付强陈静
Owner GUANGXI NORMAL UNIV
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