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A strain of Trichoderma aculeatus and its application in remediation of lead-contaminated soil

A technology for lead-contaminated soil and Trichoderma aculeatus, applied in the field of agricultural microorganisms and ecological restoration, can solve the problems of consuming large energy or chemicals, restoring the adverse effects of soil physicochemical properties of contaminated soil, and high finished products, and achieving excellent tolerance and adsorption. capacity, broad heavy metal tolerance, stress reduction effect

Active Publication Date: 2021-11-30
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the remediation technologies for lead-contaminated soil mainly include physical and chemical remediation, phytoremediation, and microbial remediation. Among them, the traditional physical and chemical remediation methods have faster results, but they require a large amount of engineering, consume a lot of energy or chemicals, etc., and the finished product is relatively high. Moreover, the use of some passivating agents may even cause the restoration of contaminated soil and have adverse effects on the physical and chemical properties of the soil, thereby affecting the subsequent use of the soil; the operation of phytoremediation is inconvenient; and the use of microbial remediation has broad application prospects

Method used

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  • A strain of Trichoderma aculeatus and its application in remediation of lead-contaminated soil
  • A strain of Trichoderma aculeatus and its application in remediation of lead-contaminated soil
  • A strain of Trichoderma aculeatus and its application in remediation of lead-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Separation and identification of strains

[0028] Separation of strains:

[0029] Select 10g from the soil of the polluting area of ​​a lead storage battery plant in Shandong, in a triangular bottle, add 90 ml of sterile water, fully oscillation to allow the microorganism to elute from the soil, then stand 20 min, absorb the supernatant 100 times diluted with sterile water; take 1 mL of the supernatant to a solid plate (Martutyrate medium) having a lead concentration of 100 mg / L, after the colony is long, and the flat line separation method is seeded to the lead concentration. In Martin's medium of 100-1500 mg / L, 30 ° C gradient domestication culture; select the strongest colonies of lead-resistance ability, purification of its single colonies continuously using flat-panel scribe separation method, screening has the best possible resistance The strain of lead capacity is a further study of the test strain.

[0030] 2. Identification of strains:

[0031] (1) M...

Embodiment 2

[0045] Example 2: aculeatus, Trichoderma (Trichoderma asperellum) determination of other heavy metals tolerance

[0046] Aculeatus, Trichoderma (Trichoderma asperellum) Example 1 Isolation inoculated on glucose peptone medium containing heavy metals (Pb, Cd, Cu and Zn), and gradually increased concentrations of heavy metals, the strain can not grow up normally, this concentration is the The minimum inhibitory concentration, the results shown in Table 1.

[0047] Table 1: Pb, Cd, Cu and Zn MIC strains of

[0048]

[0049] As can be seen from Table 1, aculeatus, Trichoderma (Trichoderma asperellum) of the present invention have very good tolerance to heavy metals Pd, while, on the heavy metals Cd, Cu, Zn, also have a certain tolerance.

Embodiment 3

[0050] Aculeatus, Trichoderma (Trichoderma asperellum) adsorption capacity and removal of lead ions measured: Example 3

[0051] In 100ml of a solution containing lead, lead concentrations were 50,100,150,200,250,300,350,400,450,500mgL -1 Conical flask were added aculeatus, Trichoderma (Trichoderma asperellum) Example 1 Isolation of 0.2g embodiment, at 150rpm min -1 , 30 deg.] C shaking at a constant temperature 24h, the solution was filtered after the completion of the shock, the lead content of the solution was measured.

[0052] Removal rate and amount of adsorption of Trichoderma aculeatus lead ions is calculated by the following formula.

[0053] Removal efficiency (η%) = (C 0 -C E ) * 100% / C 0 ;

[0054] Adsorption amount (Q) = (C 0 -C E ) * V / 1000W;

[0055] In the formula: Q- microorganism adsorption amount of lead (mg / g); C 0 - initial concentration of lead ions (mg / L); C E - equilibrium adsorption of lead concentration (mg / L); V- volume of solution (mL); mass W...

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Abstract

The invention discloses a strain of Trichoderma asperellum. The strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 22, 2018, and its biological preservation number is: CGMCC NO.15266. The trichoderma asperellum (Trichoderma asperellum) screened and isolated by the present invention has excellent tolerance and adsorption capacity to various heavy metals, especially lead, and is easy to colonize in soil polluted by heavy metals, especially in soil polluted by lead. Survival, suitable for microbial remediation of soil.

Description

Technical field [0001] The present invention relates to the field of agricultural microorganisms and ecological restoration technologies, and therefore referring to a ratcosputum miner and its application in lead contamination soil repair. Background technique [0002] Lead is a wide range of environmental pollutants in heavy metal contamination soils and strong accumulation. There are different levels of lead pollution in all parts of our region, and lead poisoning events have also occurred frequently. There is lead pollution in environmental elements such as atmosphere, water body and soil, but compared to atmospheric and water bodies, lead pollution in soil is more common. Most of the lead in the soil is present in the surface of the surface, and hardly move. Lead is easy to bind to organic matter in soil, which is extremely difficult to dissolve. Excessive lead will block crop growth and development, mainly manifested as a decrease in chlorophyll, obstructing the breathing of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10A01N63/38A01P21/00C12R1/885
CPCA01N63/30B09C1/10C12N1/14C12N1/145C12R2001/885
Inventor 诸葛玉平孙鑫娄燕宏崔秀敏宋付朋王会韩飞王婷婷
Owner SHANDONG AGRICULTURAL UNIVERSITY
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