A strain of Trichoderma aculeatus and its application in remediation of lead-contaminated soil
A technology for lead-contaminated soil and Trichoderma aculeatus, applied in the field of agricultural microorganisms and ecological restoration, can solve the problems of consuming large energy or chemicals, restoring the adverse effects of soil physicochemical properties of contaminated soil, and high finished products, and achieving excellent tolerance and adsorption. capacity, broad heavy metal tolerance, stress reduction effect
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Embodiment 1
[0027] Example 1: Separation and identification of strains
[0028] Separation of strains:
[0029] Select 10g from the soil of the polluting area of a lead storage battery plant in Shandong, in a triangular bottle, add 90 ml of sterile water, fully oscillation to allow the microorganism to elute from the soil, then stand 20 min, absorb the supernatant 100 times diluted with sterile water; take 1 mL of the supernatant to a solid plate (Martutyrate medium) having a lead concentration of 100 mg / L, after the colony is long, and the flat line separation method is seeded to the lead concentration. In Martin's medium of 100-1500 mg / L, 30 ° C gradient domestication culture; select the strongest colonies of lead-resistance ability, purification of its single colonies continuously using flat-panel scribe separation method, screening has the best possible resistance The strain of lead capacity is a further study of the test strain.
[0030] 2. Identification of strains:
[0031] (1) M...
Embodiment 2
[0045] Example 2: aculeatus, Trichoderma (Trichoderma asperellum) determination of other heavy metals tolerance
[0046] Aculeatus, Trichoderma (Trichoderma asperellum) Example 1 Isolation inoculated on glucose peptone medium containing heavy metals (Pb, Cd, Cu and Zn), and gradually increased concentrations of heavy metals, the strain can not grow up normally, this concentration is the The minimum inhibitory concentration, the results shown in Table 1.
[0047] Table 1: Pb, Cd, Cu and Zn MIC strains of
[0048]
[0049] As can be seen from Table 1, aculeatus, Trichoderma (Trichoderma asperellum) of the present invention have very good tolerance to heavy metals Pd, while, on the heavy metals Cd, Cu, Zn, also have a certain tolerance.
Embodiment 3
[0050] Aculeatus, Trichoderma (Trichoderma asperellum) adsorption capacity and removal of lead ions measured: Example 3
[0051] In 100ml of a solution containing lead, lead concentrations were 50,100,150,200,250,300,350,400,450,500mgL -1 Conical flask were added aculeatus, Trichoderma (Trichoderma asperellum) Example 1 Isolation of 0.2g embodiment, at 150rpm min -1 , 30 deg.] C shaking at a constant temperature 24h, the solution was filtered after the completion of the shock, the lead content of the solution was measured.
[0052] Removal rate and amount of adsorption of Trichoderma aculeatus lead ions is calculated by the following formula.
[0053] Removal efficiency (η%) = (C 0 -C E ) * 100% / C 0 ;
[0054] Adsorption amount (Q) = (C 0 -C E ) * V / 1000W;
[0055] In the formula: Q- microorganism adsorption amount of lead (mg / g); C 0 - initial concentration of lead ions (mg / L); C E - equilibrium adsorption of lead concentration (mg / L); V- volume of solution (mL); mass W...
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