Repair preparation for placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and preparation method thereof
A technology of subpluripotent stem cells and stem cells, applied in the field of cell culture, can solve the problems of low content of active ingredients in the culture solution, increased skin irritation, poor cosmetic effect, etc., and achieve excellent repair function, excellent whitening, and quality. Balanced effects
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Embodiment 1
[0039]A preparation for repairing placental sub-totipotent stem cells and mesenchymal stem cell mixed culture extract, including the following components by weight: placental sub-totipotent stem cell and mesenchymal stem cell mixed culture extract 50 and filler 20; The preparation method of the mixed culture extract of totipotent stem cells and mesenchymal stem cells is as follows:
[0040] 1) Aseptically collect placental tissue, and obtain placental subtotipotent stem cells through washing, digestion and separation steps;
[0041] 2) Aseptically collect placental tissue, and obtain mesenchymal stem cells through washing, digestion and separation steps;
[0042] 3) Placental subtotipotent stem cells were divided into 2×10 4 cells / ml density, mesenchymal stem cells according to 8×10 4 cells / ml, co-inoculate in the primary serum-free culture medium, change the medium every 3 days, culture for 7 days, harvest the primary mixed cells, and collect and combine the cultivated cult...
Embodiment 2
[0048] A repairing preparation of placental subtotipotent stem cells and mesenchymal stem cell mixed culture extract, including the following components by weight: placental subtotipotent stem cell and mesenchymal stem cell mixed culture extract 70 and filler 30; The preparation method of the mixed culture extract of totipotent stem cells and mesenchymal stem cells is as follows:
[0049] 1) Aseptically collect placental tissue, and obtain placental subtotipotent stem cells through washing, digestion and separation steps;
[0050] 2) Aseptically collect placental tissue, and obtain mesenchymal stem cells through washing, digestion and separation steps;
[0051] 3) Placental subtotipotent stem cells were divided into 2×10 4 cells / ml density, mesenchymal stem cells according to 8×10 4 cell / ml density, mixed with the culture medium to obtain a mixed cell suspension, and inoculated in the serum-free culture medium; at 37°C, 5% CO 2 Cultivate in humid air, replace all the cultur...
Embodiment 3
[0057] A repair preparation of placental subtotipotent stem cells and mesenchymal stem cell mixed culture extracts, the difference from Example 2 is that the primary serum-free culture medium is based on OPTI-MEM culture medium, and also includes the following concentrations of Ingredients: 15ug / mL D-glucose, 13ug / mL soybean lecithin and 8ug / mL phenylalanine.
[0058] The passage serum-free culture fluid is based on the OPTI-MEM culture fluid, and also includes components at the following concentrations: 10ug / mL diaminoethane polyacrylamide, 13ug / mL soybean lecithin, 15ug / mL lecithin and 8ug / mL Phenylalanine.
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