Factory culture method of phellinus igniarius fruiting bodies and culture medium thereof
The technology of culture medium and Phellinus linteus, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of high price of finished products, high pollution rate, long cultivation period, etc., and achieve stable product quality, high active ingredients, and cultivation period. short effect
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Embodiment 1
[0023] Embodiment 1: Rice is the main raw material.
[0024] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.
[0025] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:
[0026] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 8 days.
[0027] Expansion culture of solid strains: the composition of solid strain expansion medium is 20g / L corn flour, 20g / L maltose, 1g / L peptone, 0.6g / L MgSO4, 1g / L KH2P04, 15g / L agar, and Measure the water, the pH is natural; after inoculating...
Embodiment 2
[0034] Embodiment 2: with millet main raw material
[0035] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.
[0036] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:
[0037] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 12 days.
[0038] Expansion culture of solid strains: the composition of solid strain expansion medium is 15g / L corn flour, 15g / L maltose, 0.8g / L peptone, 0.5g / L MgSO4, 3g / L KH2P04, 20g / L agar, The remaining water has a natural pH; after the activated...
Embodiment 3
[0045] Embodiment 3: with oat main raw material
[0046] (1) Strains: Phellinus strains, purchased from Guangdong Microbial Strain Collection Center, and stored on PDA slant medium.
[0047] (2) Seed preparation: under conditions suitable for Phellinus growth, cultivate Phellinus mycelia in liquid seed medium to logarithmic growth phase to obtain liquid Phellinus seeds; or cultivate Phellinus mycelium in solid seed medium , to obtain solid Phellinus phellidis seeds, its specific steps are as follows:
[0048] Activation of preserved strains: the solid seed medium used for the activation of strains is carrot agar solid medium, and after inoculation of slant strains, the temperature is 28°C, and dark culture is carried out for 10 days.
[0049] Expansion culture of solid strains: the composition of solid strain expansion medium is 10g / L corn flour, 10g / L maltose, 0.5g / L peptone, 0.1g / L MgSO4, 4g / L KH2P04, 25g / L agar, The remaining water has a natural pH; after the activated Ph...
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