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Lipid-decreasing functional peptide by using silkworm chrysalis as raw material

A technology for lowering blood lipids and functional peptides, which is applied in the functions of food ingredients, peptide/protein components, and medical preparations containing active ingredients, etc., can solve the problems of poor separation and purification effect of lipid-lowering peptides and achieve swelling and shrinkage Fast process, easy regeneration, high yield effect

Inactive Publication Date: 2018-06-29
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the separation and purification effect of this lipid-lowering peptide is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020]The blood lipid-lowering functional peptide prepared from silkworm chrysalis has a molecular weight of 620-1200Da. The blood-lipid-lowering functional peptide can down-regulate most lipid-forming genes, reduce lipid accumulation, and exert a blood-lipid-lowering effect. It can be developed into blood-lipid-lowering drugs, health care products and food additives.

[0021] The preparation method of the blood lipid-lowering functional peptide prepared from silkworm chrysalis, the specific steps are:

[0022] 1) Pretreatment of silkworm chrysalis powder: Add a mixture of n-hexane and petroleum ether with a volume ratio of 1:1 to silkworm chrysalis powder at a material-to-liquid ratio of 1:8 (g / mL), heat and reflux for 5 hours to obtain silkworm chrysalis protein, and set aside , due to the existence of oils and fats in silkworm chrysalis, the effect of enzymatic hydrolysis of silkworm chrysalis protein is greatly reduced, and at the same time, it will be oxidized in the enzy...

Embodiment 2

[0031] The blood lipid-lowering functional peptide prepared from silkworm chrysalis has a molecular weight of 620-1200Da. The blood-lipid-lowering functional peptide can down-regulate most lipid-forming genes, reduce lipid accumulation, and exert a blood-lipid-lowering effect. It can be developed into blood-lipid-lowering drugs, health care products and food additives.

[0032] The preparation method of the blood lipid-lowering functional peptide prepared from silkworm chrysalis, the specific steps are:

[0033] 1) Pretreatment of silkworm chrysalis powder: Add a mixture of n-hexane and petroleum ether with a volume ratio of 1:2 to silkworm chrysalis powder at a material-to-liquid ratio of 1:5 (g / mL), heat and reflux for 4 hours to obtain silkworm chrysalis protein, and set aside ;

[0034] 2) Enzymatic hydrolysis of silkworm chrysalis protein: Add distilled water to silkworm chrysalis protein powder according to the ratio of material to liquid 1:25 (g / mL), mix well, adjust t...

Embodiment 3

[0042] The blood lipid-lowering functional peptide prepared from silkworm chrysalis has a molecular weight of 620-1200Da. The blood-lipid-lowering functional peptide can down-regulate most lipid-forming genes, reduce lipid accumulation, and exert a blood-lipid-lowering effect. It can be developed into blood-lipid-lowering drugs, health care products and food additives.

[0043] The preparation method of the blood lipid-lowering functional peptide prepared from silkworm chrysalis, the specific steps are:

[0044] 1) Silkworm chrysalis powder pretreatment: Add a mixture of n-hexane and petroleum ether with a volume ratio of 1:1-2 to silkworm chrysalis powder at a material-to-liquid ratio of 1:7 (g / mL), heat and reflux for 4.5 hours to obtain silkworm chrysalis egg white, spare;

[0045]2) Enzymatic hydrolysis of silkworm chrysalis protein: Add distilled water to silkworm chrysalis protein powder according to the ratio of material to liquid 1:22 (g / mL), mix well, adjust the pH t...

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Abstract

The invention discloses lipid-decreasing functional peptide by using silkworm chrysalis as raw material. The molecular weight of the lipid-decreasing functional peptide is 620 to 1200Da. A preparationmethod of the functional peptide comprises the following steps of pretreatment of silkworm chrysalis powder: adding a mixed solution of n-hexane and petroleum ether into the silkworm chrysalis powder, heating and refluxing, so as to obtain silkworm chrysalis protein; adding distilled water into the silkworm chrysalis protein powder, uniformly mixing, adding alkaline protease and papain, then adding D-carnitine, hydrolyzing by enzyme, deactivating enzyme, cooling, and then centrifuging, so as to obtain a supernatant for further use, namely an enzyme hydrolysis liquid; filtering the enzyme hydrolysis liquid by an ultrafiltration film, so as to obtain an ultrafiltration enzyme hydrolysis liquid; sequentially performing gel column chromatography, ion exchange resin chromatography, and high performance liquid chromatography purification on the ultrafiltration enzyme hydrolysis liquid, so as to obtain the lipid-decreasing functional peptide. The lipid-decreasing functional peptide has the beneficial effects that most of lipid forming genes can be regulated down, the stacking of lipid is decreased, the lipid-decreasing function is realized, and the lipid-decreasing functional peptide canbe developed into lipid-decreasing medicines, health-care products and food additives.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction, in particular to a blood lipid-lowering functional peptide prepared from silkworm chrysalis. Background technique [0002] China is a famous ancient silk country, and the "Silk Road" is famous for it. Sericulture has been an important part of China's agriculture since ancient times. There are records of silkworm raising and spinning in my country more than 7,000 years ago. At present, 80% of the world's silk production is produced in my country. Silkworm chrysalis is rich in resources in our country. It is a high-quality protein rich in 18 kinds of amino acids. The protein in silkworm chrysalis is higher than the essential amino acids of many livestock and poultry. It is rich in nutrients that can promote growth and development, improve immunity and supplement biological organisms. Nutrients with many functions such as nutrition have huge market potential. In China, eating silkwo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/18C07K1/16A61K38/01A61P3/06A23L33/18
CPCA23L33/18A23V2200/00A61K38/00C07K1/16C07K1/18C07K1/34C07K1/36C12P21/06A23V2200/3262
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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