Method for preparation of acaudina leucoprocta collagen antioxidant peptide by enzymolysis with microbial protease

Abstract

Provided is a method for preparation of an acaudina leucoprocta collagen antioxidant peptide by enzymolysis with microbial protease. The method comprises the steps: selecting acaudina leucoprocta as araw material, and removing heavy metals and miscellaneous proteins by soaking with an acid, an alkali and EDTA, to obtain collagen; with assisting of ultrasonic waves, carrying out enzymolysis by collagenase, to obtain a collagenase solution, adding glucose or sucrose to the collagenase solution, and adjusting the pH to 6-7, to obtain an initial fermentation liquid; adding bacillus geocompostingor bacillus lichenibacillus or bacillus megigantobacilli into the initial fermentation liquid, and carrying out sealed fermentation to obtain a fermentation filtrate; carrying out freeze drying of thefermentation filtrate or removing macromolecules by a tubular membrane, and carrying out intercept classification, concentration desalting and spray drying to obtain the acaudina leucoprocta collagenpeptide; and separating and purifying the collagen peptide by a membrane separation, gel filtration and reverse-phase high-performance liquid chromatography technology, and screening to obtain the collagen antibacterial peptide product.

Description

technical field [0001] The invention relates to a method for preparing anti-oxidative peptides of collagen from black ginseng collagen by using microwave-assisted microbial proteolysis, and belongs to the technical field of preparation of protein anti-oxidative peptide biological products. Background technique [0002] East China sea black ginseng (scientific name: white anus sea sweet potato Acaudina leucoprocta ), rich in the East China Sea, its body wall is rich in collagen, with extremely high nutritional and medicinal value. However, due to its large molecular weight, it is not conducive to gastrointestinal and skin absorption. [0003] Compared with collagen, collagen peptide not only has the nutritional properties of collagen, but also has biological activities such as antioxidant, antibacterial and antitumor. The collagen of black ginseng from the East China Sea has a stable structure and tight arrangement of muscle fibers, and the hydrolysis effect of general prot...

AI Technical Summary

Problems solved by technology

The collagen of black ginseng from East China Sea has a stable structure and tight arrangement of muscle fibers, and the hydrolysis effect of general protease is poor

[0004] The complex structure of black ginseng protein makes certain groups inside the protein molecule unable to contact with the active site of the enzyme, making it difficult to react