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A fermentation method for improving calcium conversion rate and antioxidant performance in sheep bone powder enzymatic hydrolyzate

A technology of sheep bone powder enzymatic hydrolysis solution and sheep bone enzymatic hydrolysis solution, applied in biochemical equipment and methods, methods based on microorganisms, fermentation, etc., can solve problems such as low utilization, low calcium conversion rate, and low polypeptide conversion rate , to achieve the effect of promoting reproduction and enhancing the function of probiotics

Active Publication Date: 2021-08-27
SHANXI AGRI UNIV
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Problems solved by technology

[0005] In order to solve the problems of low calcium conversion rate, low polypeptide conversion rate and low utilization of bone powder enzymolysis solution, the present invention provides a fermentation method for improving calcium conversion rate and antioxidant performance in sheep bone powder enzymatic solution

Method used

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  • A fermentation method for improving calcium conversion rate and antioxidant performance in sheep bone powder enzymatic hydrolyzate
  • A fermentation method for improving calcium conversion rate and antioxidant performance in sheep bone powder enzymatic hydrolyzate
  • A fermentation method for improving calcium conversion rate and antioxidant performance in sheep bone powder enzymatic hydrolyzate

Examples

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Effect test

experiment example 1

[0018] Experimental Example 1: Screening of strains with high protease activity

[0019] 1. Lactobacillus strains and reagents: Lactobacillus helveticus ( Lactobacillus helveticu s) and Lactobacillus paracasei subsp. paracasei ( Lactobacillus paracasei subsp.paracasei ) were purchased from the Microbial Culture Collection Center of the Chinese Academy of Sciences; Lactobacillus sake ( Lactobacillus sake i) separated from Lyocarni BOM-13 starter; Lactobacillus campylobacter ( Lactobacillus curvatus ) separated from Lyocarni VBL-97 starter; Lactobacillus plantarum ( Lactobacillus plantarum ) separated from Lyocarni SHI-59 starter; Pediococcus lactis ( Pediococcus acidilactici ) and Pediococcus pentosaceus ( Pediococcus pentosaceus ) separated from Lyocarni VBM-60 starter.

[0020] Alkaline protease (Activity≥200 000 U / g) and flavor protease (Activity≥20 U / mg) are products of Solarbio; 1,1-diphenyl-2-picrylphenyl (DPPH) is a product of Sigma company; none...

experiment example 2

[0029] Experimental example 2: Response surface optimization analysis of Lactobacillus plantarum fermentation conditions

[0030] Using fresh lamb bone as raw material to prepare sheep bone meal, the mass percentage of bone meal during enzymatic hydrolysis is 12%, the amount of alkaline protease added is 4.25%, the pH is adjusted to 9, and 3.65% flavor protease is added after hydrolysis at 45 °C for 400 minutes, and pH is adjusted. To 6, 55 ℃ hydrolyzed for 240min after sterilization and enzyme treatment.

[0031] Add 1% maltose to the sheep bone enzymolysis solution, gradually increase the proportion (10%, 20%, 30%~100%) of the sheep bone enzymolysis solution in the MRS broth medium, and carry out the transplantation and domestication treatment of Lactobacillus plantarum . The domesticated strains were inoculated into the sheep bone enzymatic hydrolysate solution with 1% maltose in a certain volume, and the experiment was carried out according to the response surface design....

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Abstract

The invention belongs to the technical field of biological fermentation. In order to solve the problems of low calcium conversion rate, low polypeptide conversion rate and low utilization of bone powder enzymolysis solution, a fermentation method for improving calcium conversion rate and anti-oxidation performance in sheep bone powder enzymatic solution is provided. method. Alkaline protease enzymatically hydrolyzes sheep bone enzymatic solution, domesticated Lactobacillus plantarum ( Lactobacillus plantarum ) was added to the prepared sheep bone enzymatic solution for fermentation, the inoculation amount of Lactobacillus plantarum was 4.0%-4.5% of the volume of the sheep bone enzymatic solution, the fermentation temperature was controlled to be 37-37.5°C, and the pH value was 5-5.5. 12‑14h to obtain a fermented liquid with high calcium conversion rate and antioxidant properties. Promote the transformation of bone calcium and the production of collagen short peptides and significantly improve its in vitro antioxidant capacity. Short peptides and calcium ions in turn promote the reproduction of plantaractobacillus and enhance its probiotic function. The vitamins and exopolysaccharides produced by lactic acid bacteria make Sheep bone enzymatic solution is more nutritious.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a fermentation method for improving the calcium conversion rate and antioxidant performance in the enzymatic hydrolysis solution of sheep bone meal. Background technique [0002] Calcium in bone meal exists in the form of hydroxyapatite crystal and amorphous calcium hydrogen phosphate, and protein mainly exists in the form of collagen with triple helix structure, both of which are difficult to be absorbed and utilized by the body. While collagen is degraded into peptides and a small amount of amino acids under the action of protease, calcium in bone minerals is also released in the form of ions. Ca 2+ It is an effective calcium that can be directly absorbed by the body. As a divalent mineral nutrient, it participates in many important physiological processes such as nerve conduction and bone growth in the body. It is an essential macroelement to maint...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04C12R1/25
CPCC12P1/04
Inventor 韩克光张浩霍乃蕊庞丰平胡钰洁
Owner SHANXI AGRI UNIV
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