A kind of bifidobacterium longum and its application

A technology of Bifidobacterium longum and Lactobacillus, applied in the direction of Bifidobacterium, Applied, Lactobacillus, etc., can solve the problems of side effects, allergies, liver diseases, and failure to achieve treatment, and achieve high expression level, lower expression level, no Toxic effects

Active Publication Date: 2020-09-04
无锡特殊食品与营养健康研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drugs currently used to treat inflammatory bowel disease mainly include anti-inflammatory drugs, immunosuppressants, and antibiotics. These drugs usually only relieve the symptoms of the disease, but cannot achieve the purpose of treatment, and long-term use may cause side effects, such as allergies and Liver Disease

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  • A kind of bifidobacterium longum and its application
  • A kind of bifidobacterium longum and its application
  • A kind of bifidobacterium longum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation method of Bifidobacterium longum YS108R

[0030] The main steps of the isolation method of Bifidobacterium longum are as follows:

[0031] (1) Preparation of culture medium: MRS culture medium supplemented with 0.05% cysteine ​​hydrochloride and 100 mg / L mupirocin;

[0032] (2) After gradient dilution of the collected stool samples of healthy people, select appropriate 3 gradient dilutions and spread them on the solid plate of the above-mentioned medium, and place the plate upside down in an anaerobic incubator at 37°C for 48 hours;

[0033] (3) Pick the colonies with good wire drawing effect for streaking purification;

[0034] (4) Pick the purified colonies and inoculate them in liquid culture medium for anaerobic culture for 24 to 48 hours. Collect the fermentation broth for the determination of extracellular polysaccharides, while retaining a small amount of fermentation broth. After centrifugation to collect the bacteria, it is used for genome extraction...

Embodiment 2

[0040] Example 2: Determination of the properties of Bifidobacterium longum YS108R

[0041] Determination of the biological characteristics of Bifidobacterium longum:

[0042] (1) Observation method of bacterial characteristics: pick a small amount of colonies and apply them on a glass slide, perform Gram staining, and observe under an optical microscope. Bacterial characteristics: Gram staining is positive, it grows well under pH 3.0-8.0 environment, does not form spores, the bacterial cell is about 0.5-1.5μm×2-8μm, it is rod-shaped, rod-shaped or bifurcated Y-shaped, transmission The electron microscope observation results show that there are obvious extracellular polysaccharides on the surface of the bacteria (attached figure 1 ).

[0043] (2) Observation method of colony characteristics: streak the strain on the MRS solid medium, cultivate until a single bacteria grows, and observe its colony morphology.

[0044] Colony characteristics: round, convex colonies formed on MRS solid ...

Embodiment 3

[0051] Example 3: Relief effect of Bifidobacterium longum YS108R on the symptoms of DSS-induced colitis in mice

[0052] The main steps of mouse pathology experiment are as follows:

[0053] (1) Prepare 40 male C57 mice and randomly divide them into 5 groups: normal control group (Control), model group (Model), Bifidobacterium longum YS108R intervention group (YS108R+DSS), Bifidobacterium longum C11A10B intervention group (C11A10B+DSS) and the commercial strain Bifidobacterium lactis BB12 intervention group (BB12+DSS), each with 8 mice. The experimental protocol and the treatment of mice in each group are shown in Table 2.

[0054] (2) After culturing the above-mentioned strains for 24 hours respectively, collect the cells by centrifugation, resuspend the cells in physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made into bacterial suspension, used for gavage mice, the gavage dose is 0.2mL. On the 14th day, dextran sodium sulfate (DSS) was made into a 2....

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Abstract

The invention discloses a bifidobacterium longum and application thereof, belonging to the field of biotechnology. The bifidobacterium longum YS108R strain provided by the invention has the characteristic of producing viscous exopolysaccharide, and can obviously improve the colitis model of mice induced by DSS. The strain has a good ability to tolerate simulated gastrointestinal fluid, can significantly reduce the disease activity index of mice during DSS induction, and has an effective protective effect on colon tissue. Moreover, the Bifidobacterium longum YS108R of the present invention is isolated from the intestinal flora of healthy people, has no toxic and side effects on the human body, and has certain advantages over traditional drug treatment. The bacterial strain can be used to make probiotic bacteria powder, fermented milk, etc., and has broad market prospects.

Description

Technical field [0001] The invention relates to a Bifidobacterium longum and its application, and belongs to the field of biotechnology. Background technique [0002] Inflammatory bowel disease (IBD) affects the health of millions of people every year and seriously affects the quality of life of patients. IBD is a type of chronic inflammation of the intestinal tract, which has the characteristics of recurrent episodes. It mainly includes two phenotypes of ulcerative colitis (UC) and Crohn's disease (CD). At present, the pathogenesis of IBD is not yet clear, but more and more studies have shown that the pathogenesis of IBD is related to factors such as susceptibility genes, environmental factors, immune response disorders, intestinal flora imbalance, and intestinal barrier damage. Intestinal flora is an important part of the human body. The interaction between intestinal microbes and intestinal mucosal immunity affects the initiation and regulation of immune responses. Disturban...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/127A23C9/13A61K35/745A61P1/00C12R1/01
CPCA23C9/127A23C9/1307A23L33/135C12N1/04C12N1/20A23V2002/00A61K2035/115C12R2001/01C12N1/205A23V2400/123A23V2400/249A23V2400/533A23V2200/32
Inventor 陈卫杨波赵建新王刚闫爽张灏
Owner 无锡特殊食品与营养健康研究院有限公司
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