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Preparation method of alfalfa extract

A technology of alfalfa extract, which is applied in the field of preparation of alfalfa extract, can solve the problems of unsuitability for industrial production, high requirements for production equipment, high production cost, etc., and achieve the improvement of utilization value and economic added value, and complete solid-liquid separation , The effect of reducing production conditions and production costs

Inactive Publication Date: 2018-06-26
QINGYANG DUNBO TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent CN 1916014 A discloses "a kind of alfalfa extract and its preparation method and application". The alfalfa extract is obtained by the method of ethanol reflux extraction, macroporous adsorption resin adsorption, and ethanol elution. The production cost is high and the extraction time is long , not suitable for industrial production
Chinese patent CN 102911146 A discloses "a method for extracting alfalfa from alfalfa", ethanol reflux extraction, high-speed countercurrent chromatographic column separation method to obtain alfalfa extract, the extraction cost is high, and the requirements for production equipment are relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A preparation method of alfalfa extract, comprising the following steps:

[0015] 1) Take fresh alfalfa, wash it with clean water, cut it into 1 cm pieces, add 10 times the amount of water, add cellulase, enzymolyze it at 45°C for 5 hours, add compound industrial alkali to adjust the pH to 9, and add alkaline protease , enzymatic hydrolysis at 50°C for 3 hours;

[0016] 2) Add 2% activated carbon to the obtained enzymatic hydrolysis solution, add it to a butterfly centrifuge, and centrifuge at a speed of 5600r / min for 5 minutes;

[0017] 3) Take the centrifuged supernatant and add 3% sodium sulfate solution, and stir for 10 minutes to produce an insoluble precipitate;

[0018] 4) Filtrate the precipitate, add petroleum ether to the clear liquid for extraction, remove the chlorophyll, and dry the lower phase of the extraction to obtain the alfalfa extract.

Embodiment 2

[0020] A preparation method of alfalfa extract, comprising the following steps:

[0021] 1) Take fresh alfalfa, wash it with water, cut it into 1.5 cm pieces, add 12 times the amount of water, add cellulase, enzymatically hydrolyze at 48°C for 5.5 hours, add compound industrial alkali to adjust the pH to 9.5, and add alkaline protease , enzymatic hydrolysis at 52°C for 3.5 hours;

[0022] 2) Add 2% activated carbon to the obtained enzymatic hydrolysis solution, add it to a butterfly centrifuge, and centrifuge at a speed of 5900r / min for 6 minutes;

[0023] 3) Take the centrifuged supernatant and add 4% sodium sulfate solution, and stir for 13 minutes to produce an insoluble precipitate;

[0024] 4) Filtrate the precipitate, add petroleum ether to the clear liquid for extraction, remove the chlorophyll, and dry the lower phase of the extraction to obtain the alfalfa extract.

Embodiment 3

[0026] A preparation method of alfalfa extract, comprising the following steps:

[0027] 1) Take fresh alfalfa, wash it with water, cut it into 2 cm pieces, add 15 times the amount of water, add cellulase, enzymolyze it at 50°C for 6 hours, add compound industrial alkali to adjust the pH to 10, and add alkaline protease , enzymatic hydrolysis at 55°C for 4 hours;

[0028] 2) Add 2% activated carbon to the obtained enzymatic hydrolysis solution, add it to a butterfly centrifuge, and centrifuge at a speed of 6200r / min for 8 minutes;

[0029] 3) Take the centrifuged supernatant and add 5% sodium sulfate solution, stir for 15 minutes to produce an insoluble precipitate;

[0030] 4) Filtrate the precipitate, add petroleum ether to the clear liquid for extraction, remove the chlorophyll, and dry the lower phase of the extraction to obtain the alfalfa extract.

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Abstract

The invention discloses a preparation method of alfalfa extract and belongs to the technical field of agricultural product processing. The method comprises the following steps: enzymolysis, centrifugation, precipitation, extraction and the like. The method disclosed by the invention is simple in operation, has relatively low requirements on production equipment, solves the problems of surplus yield of alfalfa and low resource utilization rate, and improves the utilization value and economic added value of the alfalfa.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a method for preparing an alfalfa extract. Background technique [0002] Medicago sativa, Rosacea, Leguminosae, perennial herb of the genus Medicago, 30-100 cm high, flowering period May-July, fruit period June-August. The roots are strong, deep into the soil layer, and the root neck is well developed. The stems are erect, clustered or even recumbent, quadrangular, glabrous or slightly pilose, with lush branches and leaves. Seeds ovoid, 1-2.5mm long, smooth, yellow or brown. It is native to Asia Minor, Iran, and Transcaucasus. It is cultivated or in a semi-wild state all over the world. It is widely planted as feed and pasture in Eurasia and countries around the world. [0003] The stems and leaves of alfalfa are tender and delicious. No matter for green feeding, silage, prepared green hay, processed grass powder, compound feed or mixed feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
Inventor 房世平秦国鹏王银
Owner QINGYANG DUNBO TECH DEV CO LTD
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