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Preparation method of silkworm chrysalis collagen

A technology of collagen and silkworm chrysalis, applied in the field of preparation of silkworm chrysalis collagen, to achieve the effects of promoting hydrolysis, ensuring stability, and high yield

Inactive Publication Date: 2018-06-22
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And prepare collagen with silkworm chrysalis, there is no report yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of silkworm chrysalis collagen comprises degreasing, enzymatic hydrolysis, salting out, dialysis, purification, and its specific steps are:

[0023] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:6 (g / mL), then degrease at 35°C for 30 minutes, filter with suction after cooling, the filter residue is degreasing silkworm chrysalis, and then bake at 50°C Dry and ready for use. This degreasing step has the advantages of high degreasing rate, no solvent residue, non-toxic and non-flammable, and no deformation of protein. At the same time, the degreasing agent is easy to recycle, which can reduce the cost of degreasing;

[0024] 2) Enzymolysis: add phosphate buffer solution with a pH of 7.5 to the defatted silkworm chrysalis at a material-to-liquid ratio of 1:30 (g / mL), then add neutral protease with 4% weight of the defatted silkworm chrysalis, and enzymatically hydrolyze at 45°C for 60 mi...

Embodiment 2

[0032] The preparation method of silkworm chrysalis collagen comprises degreasing, enzymatic hydrolysis, salting out, dialysis, purification, and its specific steps are:

[0033] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the material-liquid ratio of 1:4 (g / mL), then degrease at 45°C for 20 minutes, suction filter after cooling, the filter residue is degreased silkworm chrysalis, and then bake at 70°C dry, spare;

[0034] 2) Enzymolysis: add phosphate buffer solution with a pH of 8.5 to the defatted silkworm chrysalis at a material-to-liquid ratio of 1:20 (g / mL), then add 2% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze at 55°C for 50 minutes , then raise the temperature to 100°C, keep it warm for 10 minutes to inactivate the enzyme, and after cooling, centrifuge for 10 minutes in a centrifuge with a temperature of 5°C and a speed of 8000 rpm, and the supernatant is the crude protein liquid;

[00...

Embodiment 3

[0042] The preparation method of silkworm chrysalis collagen comprises degreasing, enzymatic hydrolysis, salting out, dialysis, purification, and its specific steps are:

[0043] 1) Degreasing: add degreasing agent to silkworm chrysalis powder according to the ratio of material to liquid 1:5 (g / mL), then degrease at 40°C for 25 minutes, filter after cooling, the filter residue is degreasing silkworm chrysalis, and then bake at 60°C dry, spare;

[0044] 2) Enzymolysis: add phosphate buffer solution with a pH of 8.0 to the defatted silkworm chrysalis at a material-to-liquid ratio of 1:25 (g / mL), then add 3% neutral protease by weight of the defatted silkworm chrysalis, and enzymatically hydrolyze at 50°C for 55 minutes , then raise the temperature to 95°C, keep it warm for 15 minutes to inactivate the enzyme, and after cooling, centrifuge for 9 minutes in a centrifuge with a temperature of 2°C and a speed of 9000 rpm, and the supernatant is the crude protein liquid;

[0045] 3)...

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Abstract

The invention discloses a preparation method of silkworm chrysalis collagen. The preparation method comprises the following concrete steps: adding a degreasing agent into silkworm chrysalis meal for degreasing, carrying out suction filtration, and drying filter residues; adding a phosphate buffer solution and neutral protease into the degreased silkworm chrysalis, carrying out enzymolysis and enzyme deactivation, and cooling and then centrifuging to obtain supernatant, i.e., crude protein liquid; adding a chitosan solution into the crude protein liquid, stirring, centrifuging supernatant and putting into an ice-water bath, adding ammonium sulfate, stirring until complete dissolution, standing overnight, centrifuging, dissolving precipitate in the phosphate buffer, carrying out dialysis, enabling dialyzate to enter a hydroxyapatite column body, adding a buffer solution for eluting, and freeze-drying to obtain the silkworm chrysalis collagen. The preparation method of silkworm chrysaliscollagen is simple and feasible, and high in degreasing efficiency; the collagen is high in yield, purity and activity, and has more concentrated molecular weight; the method is suitable for industrial production and higher in safety, and increases the additional value of the silkworm chrysalis.

Description

technical field [0001] The invention relates to the technical field of deep processing of silkworm chrysalis, in particular to a preparation method of silkworm chrysalis collagen. Background technique [0002] Collagen, or collagen, is a biological polymer synthesized by animal cells. It is widely found in animal bones, tendons, cartilage, skin and other connective tissues. It has the functions of supporting organs and protecting the body. 30% of protein. There are many kinds of aquatic animals in the ocean, and different aquatic animals have huge differences in their morphology and body structure. Squid, an invertebrate cephalopod, has a well-developed dermis, and the collagen in its body is mainly distributed in the dermis. Collagen has a stable molecular structure, low immunogenicity, and good biocompatibility. These properties determine its use. Collagen has a wide range of uses. Products have various shapes and uses, and are used in many fields such as food, chemical,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/30
CPCC12P21/06C07K14/78
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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