Preparation method of quality control serum for quality control of centrifugal microfluidic chips

Abstract

The invention discloses a preparation method of quality control serum for quality control of centrifugal microfluidic chips. The method comprises the following steps: adding 0.6 to 1.0 percent of cholesteryl sodium sulfate into bovine plasma, and adding 0.02 to 0.06 percent of monopotassium phosphate; sequentially adding 0.04 to 0. 08 percent of ammonium ferric sulfate dodecahydrate, 0.5 to 0.9 percent of calcium chloride, 0.2 to 0.6 percent of bitter salt, 0.6 to 1 percent of urea, 9 to 13 percent of sodium chloride, 0.01 to 0.03 percent of zinc vitriol, 0.01 to 0.03 percent of chalcanthite,0.06 to 0.2 percent of glycocholic acid, 2 to 4 percent of glucose, 0.03 to 0.06 percent of creatinine, 0.08 to 0.2 percent of uric acid, and 0.07 to 0.11 percent of triolein into water; uniformly mixing obtained solution; adding glycol, saccharose and triton X-100, and uniformly mixing; sequentially adding albumin bovine serum, sodium azide, alanine aminotransferase, aspartic transaminase, alkaline phosphatase, lipase and creatine kinase, uniformly mixing, and performing freeze drying. The method has the advantages that the source of the raw materials is sufficient, the raw materials are easyto get, possible matrix effect is furthest avoided, and precipitation of the raw materials is prevented.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a method for preparing a plasma quality control product used for quality control of a centrifugal microfluidic chip. Background technique [0002] Medical examination is an important part of clinical medicine. The test results are important data support for clinicians in the work of patient disease diagnosis, treatment monitoring, and prognosis judgment, and are an indispensable means in modern medicine. In my country, with the rapid development of the national economy, the continuous progress of the society, the increase of the total population and the improvement of the quality of the population, the public pays more and more attention to their own health. higher requirement. In the field of medical testing, with the continuous introduction of new technologies, new concepts and new thinking, there are two major development trends in medical testing technolog...

AI Technical Summary

Problems solved by technology

At present, hospitals above the middle level have good software and hardware facilities, but basic medical institutions, especially health centers, clinics and other units, generally lack automatic biochemical testing equipment and facilities, and there are not enough testing professionals

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