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Kit for determining content of intact parathyroid hormone and test method thereof

A parathyroid hormone and kit technology, applied in the field of biochemistry, can solve problems such as the lack of accuracy detection methods, and achieve the effects of improved precision, improved accuracy and detection efficiency, and stable process

Inactive Publication Date: 2018-06-12
JIANGSU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Intact PTH is the most primitive parathyroid hormone, it has physiological activity and is not disturbed by diseases other than the parathyroid gland. Therefore, it can truly distinguish whether abnormal blood calcium is related to the parathyroid gland. It is widely used clinically, but it is currently lacking in clinical practice. Highly accurate detection method

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  • Kit for determining content of intact parathyroid hormone and test method thereof

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Embodiment Construction

[0044] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0045] The operation procedure of adopting the test kit test sample of the present invention is as follows:

[0046] 1. Sample collection

[0047] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0048] 2. Preparation before experiment

[0049] ① Take a bottle of lotion a...

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Abstract

The invention discloses a kit for determining the content of intact parathyroid hormone antibody (iPTH) in human serum by adopting a magnetic particle-based chemiluminescence method. The kit consistsof a calibration material, a control material, an antibody reagent, a magnetic particle reagent, and a luminescent substrate; the antibody reagent is a fluorescein isothiocyanate-labeled intact parathyroid hormone-coated antibody and an alkaline phosphatase-labeled intact parathyroid hormone-labeled antibody; and the magnetic particle reagent is magnetic particles and a goat anti-FITC (anti-fluorescein isothiocyanate) adapter. The invention combines the chemiluminescence technology with immunomagnetic particles, and provides a reaction system which is close to the homogenous phase; moreover, because a one-step reaction mode is adopted, the sensitivity and precision of assay are greatly increased, the assay range is broadened, the reaction time is greatly shortened, and the time is less than 25 minutes from the beginning of sample addition to an assay result, so the kit is obviously rapider than other similar kits; furthermore, a plurality of samples can be simultaneously assayed on a full-automatic chemiluminescence instrument, the high-throughput rapid determination of the intact parathyroid hormone antibody is realized, the accuracy is high, the specificity is high, and accuracyand assay efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of whole parathyroid hormone (iPTH) in a human body by using a magnetic particle chemiluminescence method. Background technique [0002] Parathyroid hormone (PTH) can help the body maintain a stable calcium ion level. Calcium, PTH, vitamin D, phosphorus (phosphate), and magnesium together form the feedback loop. Once this feedback mechanism is destroyed by certain pathological conditions or diseases, it will lead to an increase or decrease in blood calcium and PTH levels, thereby causing hypocalcemia or hypercalcemia. [0003] PTH is produced by the 4 parathyroid glands located behind the thyroid gland. Under normal circumstances, when the body has hypocalcemia, the parathyroid gland will secrete PTH into the blood circulation. Subsequently, PTH can raise blood calcium to normal level through three ways, which are promoting the release of c...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/78
CPCG01N21/76G01N33/78G01N2333/635
Inventor 金鑫刘振世汪丹刘振鹏邹亚伟
Owner JIANGSU ZECEN BIOTECH CO LTD
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