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Fluorescence probe capable of visually inspecting firearms residues and preparation method and application of fluorescence probe

A technology of shooting residues and fluorescent probes, which is applied in the field of chemical analysis of trace evidence in forensic science, achieving the effect of simple synthesis route, low cost and high sensitivity

Active Publication Date: 2018-06-12
CHINA CRIMINAL POLICE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the use of fluorescent probe method for the detection of shooting residues, which has great application prospects

Method used

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  • Fluorescence probe capable of visually inspecting firearms residues and preparation method and application of fluorescence probe
  • Fluorescence probe capable of visually inspecting firearms residues and preparation method and application of fluorescence probe
  • Fluorescence probe capable of visually inspecting firearms residues and preparation method and application of fluorescence probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Synthesis and characterization of ratio fluorescent probe for determination of shooting residue

[0035] 7-(Diethylamino)-3-formylcoumarin (122mg, 0.6mmol) and 2-(3-cyano-4,5,5-trimethylfuran-2-ene)propanedicyanide ( 1g, 0.4mmol) was dissolved in absolute ethanol (5.0mL). NaOH (1-2 mg) was added to the solution at 80°C and stirred for 1 hour. The resulting solid was filtered and washed with cold ether. The residue was recrystallized from methanol to obtain the desired product as a dark blue solid, which is the fluorescent probe I of the present invention, 110.8 mg (65%). 1 H-NMR (300MHz, CDCl 3 ): δ7.94(s,1H, Ar-H),7.92(d,J=15.4Hz,1H,-CH δ =CH-),7.40(d,J=9.6Hz,1H,Ar-H),7.38(d, J=15.4Hz,1H,-CH δ =CH-),6.70(dd,J=2.6Hz,J=9.6Hz,1H,Ar-H),6.51(d,J=2.6Hz,1H,Ar-H),3.51(q,J=7.2Hz ,4H,-CH 2 CH 3 ),1.73(s,6H,-CH 3 ),1.28(t, J=7.2Hz,6H,-CH 2 CH 3 ),ppm.MS:[M+1] + = 427.28.

[0036] 3-formyl coumarin 343 (161mg, 0.6mmol) and 2-(3-cyano-4,5,5-trimethylfuran-2-ene)propaned...

Embodiment 2

[0037] Example 2 Fluorescent probe recognition S 2- Selective UV-Visible spectrum.

[0038] Dissolve fluorescent probes I and II in PBS buffer (10mmol / L, pH=7.4, 90% DMSO) to prepare a solution of 10μmol / L, fluorescent probe I has a strong absorption peak at 596nm; fluorescent probe II has a strong absorption peak at 611nm, which is 15nm red-shifted than fluorescent probe I. Add 50mol / L of S to the solution 2- , After similar analytes and primer ions (similar analytes include: F - , Cl - , Br - , I - , SCN - , CH 3 COO - ; Primer ion components include: N 3 - , Pb 2+ , ClO 3 - , K + , Ti 3+ , NO 3 - , Ba 2+ ), only to join S 2- Then, the absorption peak of fluorescent probe I near 596nm disappeared, and two strong absorption peaks appeared near 342nm and 398nm; the absorption peak of fluorescent probe II near 611nm disappeared, and one near 445nm appeared. Strong absorption peak; and the color of the solution changes from dark blue to yellow. However, there is no significant dif...

Embodiment 3

[0039] Example 3 Fluorescent probe recognition S 2- Selective fluorescence spectrum.

[0040] The fluorescent probe I / II was dissolved in PBS buffer (10mmol / L, pH=7.4, 90% DMSO) to prepare a solution of 10μmol / L, the fluorescent probe I appeared a strong emission peak at 678nm; Needle II has a strong emission peak at 710nm, which is 32nm red-shifted from fluorescent probe I. Add 50μmol / L of S to the solution 2- , After similar analytes and primer ions (similar analytes include: F - , Cl - , Br - , I - , SCN - , CH 3 COO - ; Primer ion components include: N 3 - , Pb 2+ , ClO 3 - , K + , Ti 3 + , NO 3 - , Ba 2+ ), only to join S 2- After that, the emission peak of fluorescent probe I at 678nm disappeared; a new emission peak appeared at 500nm; the emission peak of fluorescent probe II at 710nm disappeared, and a new emission peak appeared at 511nm; in addition, use 365nm Observation under the irradiation of ultraviolet light shows that the fluorescent color changes from red to ligh...

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PUM

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Abstract

The invention belongs to the field of chemical analysis of forensic science microphysical evidence, and particularly relates to a fluorescence probe capable of visually inspecting firearms residues and a preparation method and application of the fluorescence probe. The molecular structure of the fluorescence probe is as shown in formula I or formula II, and is used as application of visual inspection of the firearms residues. By the probe, the firearms residues are detected by colorimetric and ratio methods. The fluorescence probe capable of visually inspecting firearms residues has the advantages of good selectivity, high sensitivity, small use amount of samples, obvious visual imaging and the like, and the application prospect is wide.

Description

Technical field [0001] The invention belongs to the field of chemical analysis of forensic science trace material evidence, and specifically relates to a fluorescent probe for visual inspection of shooting residues, and a preparation method and application thereof. Background technique [0002] Globally, the number of gun-related cases is increasing. The characteristics of such cases are continuous crimes, frequent crimes, long-term harm, and wide impact. They pose a great threat to citizens' lives and property safety and social stability and harmony. Shooting residue (GSR) refers to the soot produced by the firing charge and propellant explosion during shooting, the unfinished powder particles, gun oil, metal particles, etc. These components contain a large amount of inorganic ions, such as lead ions, antimony ions and Sulfide ions, etc. It is one of the important material evidences in gun-related cases, and it has the characteristics of potential, universality, and difficulty ...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07D407/06C07D491/16G01N21/64
CPCC07D407/06C07D491/16C09K11/06C09K2211/1029C09K2211/1088G01N21/643
Inventor 李宏达吕荫妮贾儒林李继民赵鹏程王岩李心倩
Owner CHINA CRIMINAL POLICE UNIV
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