Method for extracting collagen from squid skin
A technology of collagen and squid skin, applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve the problem of weak disinfection ability of potassium permanganate, high price of potassium permanganate, and high chroma of factory water Advanced problems, to achieve the effect of shortening the decolorization time, good decolorization performance and adsorption performance, and no corrosion of equipment
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Embodiment 1
[0014] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:
[0015] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 0.8‰ according to the ratio of material to liquid at 1:9, and the NaOH aqueous solution contains 0.6% Activated carbon was ultrasonically soaked for 2 hours at an ultrasonic power of 150W and a temperature of 5°C, washed with water to neutrality, and finally an 8% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:24. Soak and degrease at ℃ for 10 hours, wash and drain with distilled water, and set aside;
[0016] 2) Acid extraction: Add 0.4M acetic acid solution to the pretreated squid skin at a ratio of 1:18, stir and extract for 5 hours at a rotation speed of 170r / min, centrifuge to take the supernatant, and continue the precipitation ...
Embodiment 2
[0020] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:
[0021] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 1.3‰ according to the ratio of material to liquid 1:7, and the NaOH aqueous solution contains 0.4% Activated carbon was ultrasonically soaked for 4 hours at an ultrasonic power of 200W and a temperature of 2°C, washed with water to neutrality, and finally a 12% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:20. Soak and degrease at ℃ for 15 hours, wash and drain with distilled water, and set aside;
[0022] 2) Acid extraction: add 0.6M acetic acid solution to the pretreated squid skin according to the ratio of solid to liquid at 1:15, stir and extract for 7 hours at a rotation speed of 130r / min, centrifuge to take the supernatant, and...
Embodiment 3
[0026] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:
[0027] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 1.0‰ according to the ratio of material to liquid 1:8, and the NaOH aqueous solution contains 0.5% Activated carbon was ultrasonically soaked for 3 hours at an ultrasonic power of 180W and a temperature of 4°C, washed with water to neutrality, and finally a 10% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:22. Soak and degrease at ℃ for 12 hours, wash and drain with distilled water, set aside;
[0028] 2) Acid extraction: add 0.5M acetic acid solution to the pretreated squid skin at a ratio of 1:16, stir and extract for 6 hours at a rotation speed of 150r / min, centrifuge to take the supernatant, and continue to precipitate for second...
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