Method for extracting collagen from squid skin

A technology of collagen and squid skin, applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve the problem of weak disinfection ability of potassium permanganate, high price of potassium permanganate, and high chroma of factory water Advanced problems, to achieve the effect of shortening the decolorization time, good decolorization performance and adsorption performance, and no corrosion of equipment

Inactive Publication Date: 2018-06-12
舟山富晟食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, decolorization with potassium permanganate has the following disadvantages: (1) The disinfection ability of potassium permanganate is weak, and the contact time is long; Containing manganese dioxide is easy to harden the filter material; (3) Potassium permanganate is more expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:

[0015] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 0.8‰ according to the ratio of material to liquid at 1:9, and the NaOH aqueous solution contains 0.6% Activated carbon was ultrasonically soaked for 2 hours at an ultrasonic power of 150W and a temperature of 5°C, washed with water to neutrality, and finally an 8% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:24. Soak and degrease at ℃ for 10 hours, wash and drain with distilled water, and set aside;

[0016] 2) Acid extraction: Add 0.4M acetic acid solution to the pretreated squid skin at a ratio of 1:18, stir and extract for 5 hours at a rotation speed of 170r / min, centrifuge to take the supernatant, and continue the precipitation ...

Embodiment 2

[0020] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:

[0021] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 1.3‰ according to the ratio of material to liquid 1:7, and the NaOH aqueous solution contains 0.4% Activated carbon was ultrasonically soaked for 4 hours at an ultrasonic power of 200W and a temperature of 2°C, washed with water to neutrality, and finally a 12% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:20. Soak and degrease at ℃ for 15 hours, wash and drain with distilled water, and set aside;

[0022] 2) Acid extraction: add 0.6M acetic acid solution to the pretreated squid skin according to the ratio of solid to liquid at 1:15, stir and extract for 7 hours at a rotation speed of 130r / min, centrifuge to take the supernatant, and...

Embodiment 3

[0026] The method for extracting collagen from squid skin comprises pretreatment, acid extraction, enzyme extraction, and its specific steps are:

[0027] 1) Pretreatment: thaw the squid skin, wash it with water, drain it, chop it up and then mash it, add it to the NaOH aqueous solution with a concentration of 1.0‰ according to the ratio of material to liquid 1:8, and the NaOH aqueous solution contains 0.5% Activated carbon was ultrasonically soaked for 3 hours at an ultrasonic power of 180W and a temperature of 4°C, washed with water to neutrality, and finally a 10% isopropanol solution was added to the squid skin at a material-to-liquid ratio of 1:22. Soak and degrease at ℃ for 12 hours, wash and drain with distilled water, set aside;

[0028] 2) Acid extraction: add 0.5M acetic acid solution to the pretreated squid skin at a ratio of 1:16, stir and extract for 6 hours at a rotation speed of 150r / min, centrifuge to take the supernatant, and continue to precipitate for second...

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PUM

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Abstract

The invention discloses a method for extracting collagen from squid skin. The method comprises the following steps: adding a NaOH aqueous solution in squid skin, ultrasonically soaking, then soaking and degreasing by using an isopropanol solution, cleaning, and draining; adding an acetic acid solution into the preprocessed squid skin, stirring, extracting, centrifuging, precipitating, washing by using water until the solution is neutral, drying for standby use, salting out the supernatant, dissolving by using acetic acid, dialyzing, freeze drying, and obtaining acid-soluble collagen; adding neutral protease and distilled water in acid extracted precipitates, enzymolysing, centrifuging, salting out the supernatant, dissolving by using the acetic acid, dialyzing, freeze drying, and obtainingthe enzyme-soluble collagen. The method has the beneficial effects that the extraction is simple, the industrialized production is easy to realize, the safety is higher, the extraction rate of the collagen is high, the additional value of the squid skin is greatly increased, and the market development potential is great; and the collagen obtained by the extraction method is white in color, free from fishy taste and high in product purity.

Description

technical field [0001] The invention relates to the technical field of deep processing of fish products, in particular to a method for extracting collagen from squid skin. Background technique [0002] Squid, although it is customary to call them fish, is actually not a fish, but a mollusk that lives in the ocean. Squid belongs to Molluscs, Cephalopoda, Calamariidae, commonly known as calamari. The body color is pale, the body is conical, the head is big, with light brown spots, and there are 10 tentacles in front. They often swim in groups in the ocean about 20 meters deep. It is often active in the middle and upper layers of shallow seas, with a vertical movement range of more than 100 meters. The meat is tender and the flavor is similar to abalone, but the price is very low, so it is called "poor man's abalone". Westerners call squid "devil fish" because of its dark and volatile skin; Spaniards eat more squid, and they process squid into canned food with different flav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14C07K1/30
CPCC07K14/78
Inventor 杨艳
Owner 舟山富晟食品科技有限公司
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