Polypeptide for preventing and treating diseases related to telomere dysfunction and its medical application

A technology of abnormal function and telomeres, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, respiratory diseases, etc., can solve the problem that the inhibitory effect of FBW7 is not completely clear, so as to increase and improve lung respiratory function and increase the number of , the effect of prolonging life

Active Publication Date: 2020-12-08
HANGZHOU DUANLI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In mammalian mice, loss of FBW7 function activates stem cell expansion; FBW7 is mutated in a variety of human tumors, but the inhibitory role of FBW7 in cell proliferation is not fully understood

Method used

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  • Polypeptide for preventing and treating diseases related to telomere dysfunction and its medical application
  • Polypeptide for preventing and treating diseases related to telomere dysfunction and its medical application
  • Polypeptide for preventing and treating diseases related to telomere dysfunction and its medical application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Application of TELODIN Polypeptide to Inhibit Telomere Damage, Chronic Obstructive Pulmonary Disease and Pulmonary Fibrosis

[0020] Experimental objects: Human cervical cancer cell Hela, 18 wild-type mice aged 3-6 months

[0021] Experimental method: After Hela cells were treated with multiple polypeptides (Table 2, SEQ ID No.1-21) for 4 hours, the cells were treated with 0Gy and 5Gy respectively, and after 1 hour, the cells were taken to detect telomeres by immunofluorescence combined with in situ hybridization DNA damage; 18 wild-type mice were divided into 6 groups, 3 in each group, mice were treated as follows, tracheal perfusion with TELODIN polypeptide (SEQ ID No.1, TyrGlyArgLysLysArgArgGluArgArgArgGlySerArgAsnGlyThrGluGluThrLys, name YK21) or control peptide (SEQ ID No.2, TyrGlyArgLysLysArgLgTluTluArglugArgArgGlys) , name YK21h, 1mg / kg body weight), tracheal infusion of bleomycin (3mg / kg body weight) after 4 hours, another two groups of mice were infus...

Embodiment 2

[0029] Example 2 Application of TELODIN Polypeptide to Inhibit Telomere Damage, Chronic Obstructive Pulmonary Disease and Pulmonary Fibrosis

[0030] Experimental objects: 24 wild-type mice aged 3-6 months

[0031] Experimental method: 12 wild-type mice were divided into 4 groups, with 3 mice in each group. The mice were treated as follows, and the trachea was infused with TELODIN polypeptide (SEQ ID No.25, SerArgAsnGlyThrGluGluThr, name ST8) or control peptide (SEQ ID No.24, GlySerArgAsnGlyThrGluGlu , name GE8, 1mg / kg body weight), tracheal perfusion of bleomycin (3mg / kg body weight) after 4 hours, tracheal intubation was performed on mice to detect respiratory function after 21 days; type II cells were sorted by flow cytometry, and statistics Percentage of type 2 cells; Extract lung tissue RNA, real-time quantitative PCR to detect the mRNA expression of NG2, T1α, s100a4, α-SMA and Col6a; Extract lung tissue protein, Western blot to detect the expression of FBW7, TPP1, α-SMA ...

Embodiment 3

[0033] Example 3 Application of TELODIN Polypeptide to Prevent Skin Aging, Weight Loss and Premature Death Caused by Radiation Damage

[0034] Experimental objects: 48 wild-type mice aged 2-4 months

[0035] Experimental method: 48 wild-type mice were randomly divided into 4 groups, 12 in each group. Firstly, the hair removal area on the back of all the mice is about 2×2 cm. Each group of mice was treated as follows: two groups of mice were not subjected to X-ray irradiation treatment, and TELODIN polypeptide (SEQ ID No. 19, TyrGlyArgLysLysArgArgGluArgArgArgGlySerArgAsnGlyThrGluGluThrLys, name DYK21) or control polypeptide (SEQ ID No. name AT22), for 7 consecutive days. 另两组小鼠每天在分别涂抹含有每天分别涂抹TELODIN多肽(SEQ ID No.19,TyrGlyArgLysLysArgArgGluArgArgArgGlySerArgAsnGlyThrGluGluThrLys,名称DYK21)或对照多肽(SEQ ID No.28,AlaCysThrGlySerThrGlnHisGlnCysGlyGlyGlyGlySerArgAsnGlyThrGluGluThr,图中名称AT22),四小时后进行4Gy X-ray exposure for 7 consecutive days. Photographs were taken and weighed daily. On the...

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PUM

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Abstract

The invention provides a polypeptide for inhibiting telomere damage, named TELODIN, which is selected from one of twelve polypeptides for inhibiting telomere dysfunction, including various variants and derivative structures thereof. The polypeptide of the present invention prevents and prevents telomere damage and telomere shortening under stress conditions and without stress, and achieves the result of prolonging telomere, enhancing stem cell expansion, increasing and improving lung respiratory function, increasing the number of skin hair follicles, and prolonging the telomere under stress conditions. The longevity of mice can inhibit the abnormal function of telomeres, delay and anti-aging, prolong life, and prevent lung and skin aging-related diseases and states caused by stress. By inhibiting the damage and shortening of telomeres in cells, Achieve the effects of delaying aging, preventing premature death and preventing related diseases. It can be used in the preparation of medicines for delaying aging and preventing diseases related to telomere dysfunction, including medicines for tissue and organ aging, degeneration, fibrosis, dysfunction and failure related diseases caused by telomere damage.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to polypeptides for preventing and treating diseases related to abnormal telomere function and its medical application, in particular to the use of polypeptides synthesized in vitro—that is, amino acid sequences, and the use of the polypeptides in the preparation of drugs for preventing and treating diseases related to abnormal telomere function The application of the polypeptide particularly relates to the medical application of anti-aging and prevention and treatment of related diseases by inhibiting the damage and shortening of the telomere of chromosomes in cells. Background technique [0002] Aging-related chronic diseases such as pulmonary fibrosis and skin fiber damage are caused by telomere DNA damage and cell aging. Progressive shortening of chromosomal telomere damage is a well-recognized mechanism leading to cellular senescence. Stress, DNA breaks caused by chemicals such as r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08C07K14/00A61K38/08A61K38/10A61K38/16A61P39/06
CPCC07K7/06C07K7/08C07K14/00A61K38/00A61P11/00A61P1/16C07K14/4702
Inventor 刘俊平
Owner HANGZHOU DUANLI BIOTECH CO LTD
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