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Method for separating and purifying Impatiens pritzellii var. hupehensis triterpenoid saponin monomers by using dynamic axial compression column

A technology of seven triterpenoid saponins and axial compression, which is applied in the field of biochemistry, can solve the problems of low purity of triterpenoid saponins, cumbersome separation steps, and large solvent consumption, and achieve low equipment performance requirements, high preparation efficiency, and simple operation Effect

Active Publication Date: 2018-06-01
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the purity of triterpene saponins obtained by the method for preparing triterpene saponins in industry is relatively low, and high-purity triterpene saponins can only be obtained through multi-step separation and purification, and the preparation process is complicated, the separation steps are cumbersome, and the solvent consumption is large.

Method used

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  • Method for separating and purifying Impatiens pritzellii var. hupehensis triterpenoid saponin monomers by using dynamic axial compression column
  • Method for separating and purifying Impatiens pritzellii var. hupehensis triterpenoid saponin monomers by using dynamic axial compression column
  • Method for separating and purifying Impatiens pritzellii var. hupehensis triterpenoid saponin monomers by using dynamic axial compression column

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Effect test

Embodiment 1

[0037] Dry the seven rhizomes in cold water, crush them, put them into a multifunctional extraction tank, add pure water to extract three times, each time for 3 hours, and the temperature is 80-95°C. After the extraction is completed, adjust the pH of the extract to 8, and combine the three extracts. Settled for 24 hours. After the settling is completed, take the supernatant for coarse filtration, pump the clean supernatant into the high level tank, enter the resin column filled with D101 resin for adsorption, wash the column with water after the adsorption is completed, and wash until the pH test paper is neutral . Then 4 times the resin volume of 30% ethanol was used to elute to remove impurities, and then 4 times the resin volume of 78% ethanol was used for analysis at a flow rate of 100 L / h. The ethanol was recovered from the analysis solution under vacuum and reduced pressure to obtain cold water seven total saponins fluid extract.

[0038] Dissolve the cold water hepta...

Embodiment 2

[0049] Dry the seven rhizomes in cold water, crush them, put them into a multi-functional extraction tank, add pure water to extract three times, each time for 4 hours, and the temperature is 80-95°C. After the extraction, adjust the pH of the extract to 9, combine the three extracts, and settle 24 hours. After the settling is completed, take the supernatant for coarse filtration, pump the clean supernatant into the high level tank, enter the resin column filled with D101 resin for adsorption, wash the column with water after the adsorption is completed, and wash until the pH test paper is neutral . Then 4 times the resin volume of 30% ethanol was used to elute to remove impurities, and then 4 times the resin volume of 80% ethanol was used for analysis at a flow rate of 100 L / h. The ethanol was recovered from the analysis solution under vacuum and reduced pressure to obtain cold water seven total saponins fluid extract.

[0050] Dissolve the cold water heptasaponin fluid ext...

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Abstract

The invention provides a method for preparing Impatiens pritzellii var. hupehensis triterpenoid saponin monomers by using a dynamic axial compression column. The method comprises the following steps:performing extraction on Impatiens pritzellii var. hupehensis; performing macroporous resin impurity removal and enrichment; performing primary purification on saponin active parts by using a medium-pressure C18 column; filling the dynamic axial compression column with a C18 filler, and continuously washing the dynamic axial compression column by using a mobile phase until a chromatographic instrument base line is stable; performing filtration on the diluted Impatiens pritzellii var. hupehensis saponin active ingredient sample solution subjected to medium-pressure C18 column primary purification, injecting the filtered sample solution into a balanced dynamic axial compression column, performing isocratic elution, wherein an eluent is methanol:methyl tert-butyl ether:water = (30-50%):(5-20%):(30-65%) (V / V / V), and detecting the sample separation conditions by using an ultraviolet detector; and performing identification according to the retention time and the chromatographic peak height,determining the start and stop collection time of each Impatiens pritzellii var. hupehensis triterpenoid saponin monomer, and performing reduced-pressure concentration on each collected saponin monomer solution separately to remove solvents. The method provided by the invention has a simple process, can simultaneously separate, purify and prepare a plurality of high-purity triterpene saponin compounds, and has a high utilization rate of plant raw materials.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for preparing cold-water heptapenoside monomers by using a dynamic axial compression column. Background technique [0002] Impatiens pritzllii Hook.f.var.hupehensis Hook.f. is a plant of Impatiens pritzllii Hook.f.var.hupehensis Hook.f., a plant of the genus Impatiens pritzllii. Its rhizome is a national medicine commonly used by the Tujia people in western Hubei. The effect of swelling. Folks use it to treat rheumatoid diseases, and the curative effect is very good. [0003] The main components of cold water seven are triterpenoid saponins, aliphatics, sterols, nitrogen-containing compounds, sugars, etc. Triterpenoid saponins are the main active ingredients of Lengshuiqi. The main pharmacological effects of cold water seven are: 1. Analgesic effect. Zhou Xuefeng et al. investigated the effects of 8 monomeric compounds isolated from Lengshuiqi on the number of writhing in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 张建超黄春华周琪
Owner HUBEI UNIV OF CHINESE MEDICINE
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