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Composite nanometer particle as well as preparation method and application thereof

A technology of composite nanoparticles and nano-microspheres, which is applied in wave energy or particle radiation treatment materials, drug combinations, pharmaceutical formulations, etc., can solve the problems that photosensitive liposomes cannot reach deep tissues, are not easy to synthesize, and have poor water solubility. Achieve the effect of precise targeted therapy, reduced damage, and uniform particle size

Active Publication Date: 2018-05-29
北京柏欧利诺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the reported two-photon photosensitizers are not easy to synthesize, and their water solubility is poor, and they are easy to aggregate in physiological environments, which limits their applications.
[0003] CN105997877A discloses a photosensitive liposome with oxygen-carrying enhanced photodynamic force, consisting of phospholipids, cholesterol, long-circulation materials and hemoglobin, and chemotherapeutic drugs and / or photosensitizers, each component is dissolved in an organic solvent, and the decompression spin Steam to form a film, then add oxyhemoglobin solution, or a mixed solution of photosensitizer and / or chemotherapeutics and oxyhemoglobin, hydration reaction, to obtain a multilayer liposome solution, which can be obtained by ultrasonication and purification, but the preparation of this invention Photosensitive liposomes cannot reach deep tissues, and can only perform superficial treatment, with poor effect
This is an extremely disadvantageous factor for cancer treatment

Method used

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  • Composite nanometer particle as well as preparation method and application thereof
  • Composite nanometer particle as well as preparation method and application thereof
  • Composite nanometer particle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] This example prepares composite nanoparticles with oxygen-carrying and two-photon absorption functions through the following steps:

[0086] (1) Take 20 g of the mixture of myristoylphosphatidylcholine and distearylphthalethylphosphatidylethanolamine-polyethylene glycol-folic acid in a mass ratio of 30:1, add methanol and chloroform to dissolve. Put it in a rotary evaporator and evaporate it for 40 minutes, dry it in vacuum for 4 hours, add 1 mL of aqueous solution, and hydrate in a water bath at 37°C for 1 hour. The hydrated solution was passed through a 200nm filter membrane 20 times to obtain a phospholipid compound nanosphere dispersion.

[0087] (2) Add an aqueous solution to the bispyrene solution dissolved in the dimethyl sulfoxide solvent, the concentration of the bispyrene solution is 5mM, and the volume ratio added is 1:10, after mixing evenly, put it in an ultrasonic wave for 30min to obtain Bipyrene microsphere dispersion;

[0088] (3) Add 1.5 mL of 3 mg / m...

Embodiment 2

[0116] This example prepares composite nanoparticles with oxygen-carrying and two-photon absorption functions through the following steps:

[0117] The difference from Example 1 is that the mass ratio of myristoylphosphatidylcholine to distearylphthaloylphosphatidylethanolamine-polyethylene glycol-folic acid is 20:1, and Example 2 has two-photon absorption function oxygen-loaded phospholipid nanoparticles.

Embodiment 3

[0119] This example prepares composite nanoparticles with oxygen-carrying and two-photon absorption functions through the following steps:

[0120] The difference from Example 1 is that the rotary evaporation time of the rotary evaporator is 60min, vacuum dried for 12h, and the hydrated solution is passed through a 200nm filter membrane for 15 times, and the oxygen-carrying phospholipid nanoparticles with two-photon absorption function are obtained in Example 3 .

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Abstract

The invention provides a composite nanometer particle as well as a preparation method and application thereof. The composite nanometer particle is prepared from the following ingredients including di-pyrene molecules, a photosensitizer, a phospholipid compound and a compound with oxygen carrying capability. The composite nanometer particle provided by the invention has the advantages that the particle diameter is uniform; the dispersion is uniform; the particle diameter is about 200nm; the particle diameter is adjustable; in addition, di-pyrene molecules and the photosensitizer are used for forming two-photon absorption materials. Compared with the photosensitizer in the prior art, the composite nanometer particle has the advantages that the absorption cross section is large; photon can bemore effectively absorbed; the deeper detection depth can be reached; meanwhile, the damage to the normal tissue is reduced; through the FRET (fluorescence resonance energy transfer) effect, the energy can be transferred to the photosensitizer; the cells can be more effectively killed and injured; higher application values are realized in the photodynamic treatment.

Description

technical field [0001] The invention belongs to the field of biological nanomaterials, and relates to a composite nanoparticle and its preparation method and application, in particular to a composite nanoparticle with two-photon absorption and oxygen-carrying functions, and its preparation method and application. Background technique [0002] Photodynamic therapy is a new cancer therapy that has received considerable attention in recent years. The basic process of the therapy is: the photosensitizer is excited by laser irradiation of a specific wavelength, and the photosensitizer in the excited state transfers energy to the surrounding oxygen to generate highly active singlet oxygen, singlet oxygen and adjacent biomacromolecules Oxidation reactions occur, resulting in cytotoxicity and resulting in cell damage and even death. Only cells exposed to light are damaged, making this method uniquely selective. However, currently clinically approved photosensitizing drugs can only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K9/51A61K47/24A61K47/42A61P35/00
CPCA61K9/5123A61K9/5169A61K41/008A61K49/0021A61K49/0093
Inventor 杨洋曹洪倩王磊李峻柏
Owner 北京柏欧利诺生物科技有限公司
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