Preparation method of collagen-based electrospinning fiber antibacterial agent carriers

A technology of electrospun fibers and antibacterial agents, which is applied in the field of polymer carrier preparation, can solve the problems of short release time of antibacterial agents, etc., and achieve stable and sufficient release effects

Inactive Publication Date: 2018-05-18
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars have prepared it into nanoparticles and applied it in the field of antibacterial agent transportation, but because of its fast degradation speed, there is a certain burst release phenomenon, and the short release time of antibacterial agents limits its application in the field of antibacterial agent transportation. It is also a problem to prepare lignin nanoparticles with uniform particle size and controllable size simply and efficiently

Method used

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  • Preparation method of collagen-based electrospinning fiber antibacterial agent carriers
  • Preparation method of collagen-based electrospinning fiber antibacterial agent carriers
  • Preparation method of collagen-based electrospinning fiber antibacterial agent carriers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Dissolve kraft lignin and antibacterial gemifloxacin in tetrahydrofuran at a mass ratio of 10:1 to prepare an organic solution with a kraft lignin concentration of 10 mg / ml, and pass the resulting organic solution through a 0.22 μm Filter the membrane to remove insoluble matter; slowly add the filtrate to the ultrapure water under stirring, the stirring speed is 300r / min, the volume ratio of water and lignin solution is 4:1, stir for 10min, and dissolve the liquid at 5000r / min Centrifuge for 10min, take the supernatant and put it into a 12-14KDa dialysis bag for dialysis for 2 days, and obtain uniform antibacterial agent carrier particles after freeze-drying, whose microscopic appearance is shown in figure 1 .

[0042] (2) Use an ultrasonic cell pulverizer to disperse the carrier particles prepared in step (1) evenly in deionized water at a concentration of 10mg / ml. Under the power of 600W, ultrasonicate for 1h, and polyvinyl alcohol (PVA) with a degree of polymeriz...

Embodiment 2-5

[0051] Except for the concentration of lignin in step (1), other process parameters are the same as in Example 1, and the proportion of lignin added in Examples 2-5 is shown in Table 1

[0052] Table 1 Example 2-5 lignin concentration

[0053]

[0054] Embodiment 2-5 antibacterial agent release and bacteriostasis experiment see respectively Figure 4 and 5 .

[0055] The comparison shows that with the increase of the drug-loaded lignin nanoparticles, the release rate of the antibacterial agent gradually increases, but there is no burst release phenomenon in these types; The effect increases.

Embodiment 6-8

[0057] Except for the PVA concentration in step (2), other process parameters are the same as in Example 1, and the content of PVA mass volume ratio in Examples 6-8 is shown in Table 2

[0058]

[0059] The morphology of the fiber membrane prepared in Examples 6-8 is consistent with that of Example 1, and the bacteriostasis, degradation performance, and antibacterial agent release performance are all good.

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Abstract

Provided is a preparation method of collagen-based electrospinning fiber antibacterial agent carriers. The invention relates to a method for preparing degradable macromolecular carriers of which the release is controllable. According to the method, through the four steps of preparation of carrier particles, preparation of electrospinning liquid, preparation of spinning fiber membranes and post-treatment of the fiber membranes, yellow-white electric discharging fiber membranes with good biological activity, biodegradability, antibacterial properties and excellent medicine carrying properties are obtained. The fiber membranes have good biological activity and can be used through application outside bodies or implanting inside the bodies. Raw materials used are collagen, lignin, PVP and PVA with low molecular weight, and the biodegradation of the fiber membranes can be achieved even if the fiber membranes are in a long-term service in the alkaline environment of body fluid, which can notonly ensure the stable release of antibacterial agents, but also enable the antibacterial agents to be fully and thoroughly released; simultaneously lignin nanoparticles encapsulated in spinning fibers prepared can make the fiber membranes show some antibacterial properties during the degradation of the fiber membranes.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a preparation method of a polymer carrier capable of loading antibacterial agents, in particular to a preparation method of a degradable and controllable release polymer carrier. Background technique [0002] A large number of failure cases of interventional therapy are closely related to bacterial infection. Therefore, the sterile environment during the treatment process plays an important role in the success or failure of the treatment. The development of nanotechnology provides new methods for many biomedical applications, especially the controlled delivery of antimicrobial agents. The application of antibacterial agent transport carrier can improve the treatment efficiency and reduce the chance of infection. At present, there have been related reports on nanoparticles and hydrogels as transport carriers of antibacterial agents. Among them, there is a certain burst release ...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728D01D5/00A61K9/70A61K47/42A61K47/30A61K47/32A61K31/4375A61K31/4709A61K33/34A61K33/30A61K33/38A61P31/04D06M15/15D06M101/04D06M101/24D06M101/18
CPCA61K9/7007A61K31/4375A61K31/4709A61K33/30A61K33/34A61K33/38A61K47/30A61K47/32A61K47/42D01D5/003D01D5/0061D01D5/0092D04H1/4382D04H1/728D06M15/15D06M2101/04D06M2101/18D06M2101/24
Inventor 夏晓梅李宝娥夏丹李海鹏梁春永王洪水刘帅
Owner HEBEI UNIV OF TECH
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