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Molybdenum sulfide-ferrite nano-enzyme as well as preparation and application

A technology of ferrite and molybdenum sulfide, applied in the direction of molybdenum sulfide, inorganic chemistry, iron compounds, etc., can solve the problems of complex operation and high cost, and achieve the effects of high catalytic activity, high specific surface area and strong catalytic activity

Active Publication Date: 2018-05-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional colorimetric method based on biological enzymes for the determination of peroxides, although highly sensitive, accurate and reliable, but the operation is more complicated and the cost is higher

Method used

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  • Molybdenum sulfide-ferrite nano-enzyme as well as preparation and application
  • Molybdenum sulfide-ferrite nano-enzyme as well as preparation and application
  • Molybdenum sulfide-ferrite nano-enzyme as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042](1) Weigh 5.8 mmoles of ferric chloride hydrate, 2.9 mmoles of magnesium chloride hydrate and 8.0 mmoles of dodecylamine, dissolve them in 75 ml of ethylene glycol, and stir well at room temperature;

[0043] (2) Transfer the mixed solution of step (1) into a 100ml autoclave, and react at 220°C for 20 hours;

[0044] (3) After the reaction product in step (2) is centrifuged to remove water (rotation speed: 4500 rpm), it is washed with deionized water repeatedly for 3 to 5 times until the pH value of the solution is close to 7.0, and the washed reaction product is placed at 55°C Under drying for 10 hours to obtain ferrite magnesium;

[0045] (4) Weigh 0.54 mmol of ammonium tetrathiomolybdate and dissolve it in 65 ml of dimethylformamide;

[0046] (5) Slowly add 1.0 ml of hydrazine hydrate to the mixture obtained in step (4), and stir for 0.5 hours to ensure that it is evenly mixed;

[0047] (6) Weighing 0.3 mmol of ferrite magnesium obtained in step (3), and dispersing ...

Embodiment 2

[0053] a) Disperse 1 mg of molybdenum sulfide-magnesium ferrite nanozyme in Example 1 in 1 milliliter of deionized water to obtain a 1 mg / ml molybdenum sulfide-magnesium ferrite dispersion;

[0054] b) Measure 10 microliters of the mixture in step a), 250 microliters, 2 mmol / L TMB and 100 microliters of 2.5~300 micromol / L hydrogen peroxide in 0.1 mol / L, pH 4.0 acetic acid-sodium acetate buffer;

[0055] c) incubating the mixture obtained in step b) at 45° C. for 15 minutes;

[0056] d) Utilize a spectrophotometer to measure the concentration of hydrogen peroxide in the mixed solution obtained in step c).

[0057] figure 2 It is the ultraviolet absorption spectrogram of the molybdenum sulfide-ferrite magnesium nanozyme of the present invention detecting hydrogen peroxide at different concentrations.

[0058] The method for detecting hydrogen peroxide by molybdenum sulfide-ferrite magnesium nanozyme in the present invention is further described in conjunction with specific e...

Embodiment 3

[0060] The absorbance at 652 nanometers in Example 2 is plotted with the concentration of hydrogen peroxide ( image 3 ), a linear relationship diagram can be obtained.

[0061] From image 3 It can be seen that the molybdenum sulfide-magnesium ferrite nanozyme maintains a good linear relationship to hydrogen peroxide in the range of 2.5 to 300 micromol / liter.

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Abstract

The invention relates to a molybdenum sulfide-ferrite nano-enzyme as well as a preparation method and an application method thereof. The preparation method comprises the following steps: uniformly mixing ferric chloride hydrate, magnesium chloride hydrate and dodecyl amine with a proper amount of ethylene glycol; enabling the components to react in a high-pressure reaction kettle, and repeatedly cleaning the product; drying the product so as to obtain ferrite magnesium; dissolving ammonium tetrathiomolybdate into dimethyl formamide; slowly adding hydrazine hydrate, and uniformly mixing; putting a proper amount of the ferrite magnesium into the mixed liquid; enabling the components to react in the high-pressure reaction kettle, and repeatedly cleaning the product; drying the product so as to obtain molybdenum sulfide-ferrite magnesium; putting the molybdenum sulfide-ferrite magnesium into a proper amount of TMB (Tetramethylbenzidine) and hydrogen peroxide-sodium acetate buffer solutionsof different concentrations; culturing, and testing the concentration of hydrogen peroxide. Results show that when being adopted to detect hydrogen peroxide, the molybdenum sulfide-ferrite magnesiumnano-enzyme is convenient and rapid to operate, high in sensitivity and wide in detection concentration range.

Description

technical field [0001] The invention relates to the technical field of preparation of molybdenum sulfide-magnesium ferrite nanozyme, and also relates to a method for detecting peroxide. A molybdenum sulfide-ferrite nanozyme, its preparation and application. Background technique [0002] Because of its simplicity and visualization, colorimetry has been widely used in the fields of biomass detection, immunoassay and environmental prediction. The traditional enzyme-based colorimetric method for the determination of peroxide has high sensitivity, accuracy and reliability, but the operation is complicated and the cost is high. Recently, the colorimetric method based on nanomaterials for the determination of peroxides is receiving more and more attention, because the use of "nanozymes" to replace biological enzymes not only greatly saves costs, simplifies the operation steps, but also has higher detection stability. sex. The so-called "nanozyme", that is, nanomaterials are used...

Claims

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Application Information

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IPC IPC(8): C01G49/00C01G39/06B01J27/051B01J35/02
CPCC01G39/06C01G49/0018B01J27/0515C01P2004/30C01P2004/03C01P2002/84C01P2004/80B01J35/00B01J35/30
Inventor 张娅温芳芳朱鸣王宏归周芝峰谈晶
Owner YANGZHOU UNIV
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