Kit for measuring rT3 content by magnetism particulate chemiluminescence method and detection method of kit

Abstract

The invention discloses a kit for measuring an rT3 content by a magnetism particulate chemiluminescence method and a detection method of the kit. The kit provides a preparation method of an rT3 antigenic derivative, the rT3 antigenic derivative and an enzyme-labeled rT3 antigenic derivative are prepared, and a biotinylated rT3 antigenic derivative, anti-body enzyme-labeled reagent and calibrationproduct detection system and an enzyme-labeled rT3 antigenic derivative, biotinylated rT3 rabbit polyclonal and calibration product detection system can be built. As an enzymatic luminescence system possibly needs to perform coupling under different conditions such as biotin, enzyme labels and even protein on rT3 antigens under different conditions, the rT3 antigenic derivative serving as an intermediate is prepared, and the problem can be solved. Detection value and given value correlations of different systems are 0.9 or more, good correlations are shown, the correlations of two measuring systems are 0.9540, good correlations of two testing methods are shown, and testing results are consistent, a whole reaction system is short in reaction time, radioactive pollution is avoided, a validity period of a reagent is prolonged, the kit is high in sensitivity and accurate in testing result, and test operation processes are simplified.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for measuring rT3 content by a magnetic particle chemiluminescence method and a detection method thereof. Background technique [0002] T3 is an important indicator reflecting thyroid function, which is formed by the removal of an iodine from T4. The structure of rT3 and T3 is basically the same, except that the positions of three iodine atoms are different from T3, at the 3, 3', 5' positions, and in the peripheral tissues ( Such as liver, kidney, etc.) are generated by T4 through the action of 5-deiodinase. rT3 is also an indicator reflecting thyroid function. Serum T4, T3 and rT3 maintain a certain ratio, which can reflect the metabolism of thyroid hormones in the body. [0003] rT3 and T3 are isomers in chemical structure, but T3 is an important hormone involved in the body's metabolism, the process consumes oxygen, while rT3 has almost no physiological activity. The increase of rT3...

AI Technical Summary

Problems solved by technology

[0015] The technical problem to be solved in the present invention is to overcome the luminescent system (enzymatic chemiluminescence) that most of the existing rT3 kits adopt alkaline phosphatase (ALP) or horseradish peroxidase (HRP), but rT3 is directly coupled The method is too difficult or impossible to provide a highly versatile kit that uses rT3 derivatives as raw materials and couples them as anti-reagent components

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