Hexavalent-chromium reduction immobilization bacterial agent and preparing method

Abstract

The invention discloses an immobilized microbial hexavalent-chromium removing bacterial agent and a preparing method, and belongs to the technical field of chromium pollution site microbial remediation. The bacterial agent is prepared in the mode that calcium alginate serves as a carrier and is embedded with independently-separated wild bacillus. The preparing method includes the steps that an embedding agent and microbial bacterial suspension are evenly mixed according to the proportion of 1:1, the obtained thick mixed liquid is dropped into a CaCl2 solution and is dispersed into balls, coupling is carried out for a certain time, and the gel-state immobilization hexavalent-chromium removing bacterial agent can be obtained. The hexavalent-chromium reduction immobilization bacterial agent is compared with a free chromium reducing bacterium, the removing efficiency of hexavalent chromium can be remarkably improved, and the removing rate can be 100% at 96 h. According to the hexavalent-chromium reduction immobilization bacterial agent prepared with the method, the preparing method is simple and easy to operate, the reaction condition is mild, and the activity and the concentration ofmicrobial bacterial suspension can be efficiently kept; the formed bacterial agent is good in repeatability and stability, the microbial bacterial suspension is not prone to leakage, solid-liquid separation after reacting is facilitated, and the hexavalent-chromium reduction immobilization bacterial agent and the preparing method are suitable for industrial application.

Description

technical field [0001] The invention belongs to a hexavalent chromium-contaminated site (waste water, waste residue, polluted soil, etc.) microbial remediation, reduction and immobilization bacterial agent and a preparation method thereof, in particular to a hexavalent chromium reduction and immobilization bacterial agent and a preparation method thereof. Background technique [0002] Chromium is a widely used transition heavy metal with numerous applications in electroplating, leather tanning, wood protection, textile dyeing and metal processing industries. The most common chromium compounds in the atmosphere, soil and water exist as Cr(VI) or Cr(III). Cr(III) is an essential micronutrient element for organisms, which cannot penetrate the cell membrane and is relatively insoluble in water; compared with Cr(III), Cr(VI) has high mobility and high water solubility, and is easy for all organisms Body produces carcinogenicity, teratogenicity, mutagenicity. According to EPA an...

AI Technical Summary

Problems solved by technology

In this case, if the industrial chromium-containing wastewater waste cannot be effectively resolved, it may lead to the pollution of natural water sources and eventually threaten human health

[0003] The conventional way to remediate Cr(VI) pollution is to use physical / chemical remediation, such as chemical reduction, adsorption, reverse osmosis, electrochemical treatment, etc. However, these methods are expensive, cannot completely remove heavy metals, consume high reagents, and Will produce toxic sludge and easily cause secondary pollution

In addition, most of these methods can effectively remove toxic metals under the condition of high concentration or medium concentration of heavy metals, but they are not suitable for dealing with low concentration of heavy metal pollution such as chromium (1 ~ 100mg / L)

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