Saccharification enzyme and preparation method thereof

A saccharification enzyme and formula technology, applied in biochemical equipment and methods, glycosylase, enzymes and other directions, can solve the problems of complex saccharification enzyme production process, low conversion rate, waste and other problems

Inactive Publication Date: 2018-05-08
贵州自然禾苑生物科技有限公司
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  • Abstract
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a glucoamylase and its preparation method, using a glucoamylase and its preparation method to solve the traditional glucoamylase production process complex, low conversion rate, serious waste, how to use reasonable raw materials and Formulating high-quality glucoamylase is a technical problem at present

Method used

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Embodiment Construction

[0013] The invention is further introduced below.

[0014] A glucoamylase formula, the formula of which is: 20-50% of original ecological koji grass leaves, 10-20% of wheat bran, 3-5% of oat flour, 3-5% of glutinous rice flour, 4% of corn flour, and 0.1% of bran oil %, bean cake powder 3%.

[0015] Its manufacturing process includes the following steps:

[0016] In the first step, when the jacket is heated to 60°C, add 6u / g of the original ecological organism Curlwort, the corn flour is liquefied, stop stirring, and directly steam heat to 120°C-125°C for 40 minutes;

[0017] The second step is to cool to 30°C and insert the seed culture solution, and the inoculation amount is 15%;

[0018] Step 3: Introduce air for cultivation, keep the pressure in the seed tank at 49kpa, the air volume at 1:0.5, keep the temperature at 31°C-32°C for 24 hours, the pressure inside the fermenter at 49kpa, and the air volume at 1:(0.5-0.7) , the temperature is maintained at 31°C to 32°C, feedi...

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Abstract

The invention discloses a saccharification enzyme and a preparation method thereof. The saccharification enzyme comprises 20-50% of original ecological Anacamptis pyoamidalis leaves, 10-20% of wheat bran, 3-5 % of oat flour, 3-5 % of glutinous rice flour, 4% of corn flour, 0.1% of oil abstracted from bran and 3% of bean cake powder. Through the saccharification enzyme formula and the preparation method, the problem that the traditional saccharification enzyme production method has complex processes, a low conversion rate and serious waste. How to use reasonable raw materials and formula to produce a high-quality saccharification enzyme is a current technical problem.

Description

technical field [0001] The invention relates to an enzyme and a preparation method thereof, in particular to a glucoamylase and a preparation method thereof, belonging to the field of industrial economy. Background technique [0002] Glucoamylase, also known as glucoamylase, is a customary name, and its scientific name is α-1,4-glucohydrolase (α-1,4-Glucan glucohydrolace). It is mostly used in alcohol, starch sugar, monosodium glutamate, antibiotics, citric acid, beer and other industries, as well as liquor and rice wine. Glucoamylase is extracted from Aspergilus niger through submerged fermentation. [0003] "China Glucoamylase Market Status Survey and Development Prospect Analysis Report (2016-2022)" conducts specific investigation, research, and analysis of relevant factors in the glucoamylase industry to gain insight into the future development direction of the glucoamylase industry and the evolution of the competitive landscape of the glucoamylase industry As well as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/34
CPCC12N9/2428C12Y302/01003
Inventor 秦兴毅
Owner 贵州自然禾苑生物科技有限公司
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