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Method for extracting peroxy ergosterol from cordyceps cicadae

A technology of peroxyergosterol and obtained peroxyergosterol, applied in directions such as steroids, organic chemistry, etc., can solve problems such as an extraction method of ergosterol that has not been seen before, and achieves high yield, low extraction temperature, and extraction time. short effect

Active Publication Date: 2018-05-08
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few reports on peroxyergosterol purification separation and purification technology, and there is no report on the peroxyergosterol extraction method that is derived from Cordyceps cicadae and can reach a purity of more than 95%.

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  • Method for extracting peroxy ergosterol from cordyceps cicadae
  • Method for extracting peroxy ergosterol from cordyceps cicadae

Examples

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Embodiment 1

[0029] 1. Method 1: a method for extracting peroxyergosterol from Cordyceps cicadae, comprising the following steps:

[0030] (1) Prepare Cicada Cordyceps dry powder and add 75% ethanol aqueous solution, extract 3 times through ultrasonic wave, after extracting liquid merges, obtain peroxyergosterol crude extract through decompression concentration; The frequency of described ultrasonic wave is 30kHz, and the time is 0.5h / times, the temperature is 60°C;

[0031] (2) enrich the peroxyergosterol crude extract obtained in step (1) through a lipophilic solvent, concentrate and dry to obtain the peroxyergosterol crude extract A; the lipophilic solvent is composed of the following components by volume fraction: 70% ethyl acetate and 30% petroleum ether;

[0032] (3) dissolving the peroxyergosterol crude extract A obtained in step (2) with 70% ethanol aqueous solution, and collecting the peroxyergosterol flow fractions of 70~90% ethanol after separation and purification of D101 mac...

Embodiment 2

[0039] Example 2 The analysis of peroxyergosterol content in different parts of Cordyceps cicadae

[0040] The content of peroxyergosterol in different parts of Cordyceps sinensis was analyzed: Cordyceps sinensis was divided into three parts: base, spore bundle and spore powder. After the three parts were crushed respectively, they were extracted 3 times by ultrasonic method (at 60 °C Under the condition of water bath, carry out ultrasonic extraction respectively with 75% ethanol aqueous solution), the extracts are combined and concentrated under reduced pressure to obtain the extracts of pedestal extracts, spore bundles extracts and spore powder extracts respectively. Product control analysis of the content of peroxyergosterol in each extract, the results are as shown in Table 1, the spore bundles of Cordyceps cicadae contain abundant peroxyergosterol compounds.

[0041] Table 1 Content of peroxyergosterol in different parts of Cordyceps cicadae

[0042]

Embodiment 3

[0044] A method for extracting peroxyergosterol from the spore bundles of Cordyceps sinensis, comprising the following steps:

[0045] 1. Prepare the extract of the spore bundle part

[0046] Pulverize the spore bundles, add 75% ethanol aqueous solution, and extract 3 times by ultrasonic waves, the frequency of the ultrasound is 30kHz, the time is 0.5h / time, and the temperature is 60°C. After the extracts are combined, they are concentrated under reduced pressure to obtain the spore bundles Extract extract.

[0047] After analysis, the content of peroxyergosterol in the extract reaches 16.5%.

[0048] 2. Lipophilic solvent enrichment peroxyergosterol

[0049]The extract obtained in step 1 is enriched with a lipophilic solvent, concentrated and dried to obtain peroxyergosterol crude extract A; the lipophilic solvent is composed of the following components by volume fraction: 70% ethyl acetate and 30% petroleum ether . After analysis, the peroxyergosterol content in the pero...

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Abstract

The invention belongs to the technical field of extraction and separation, in particular discloses a method for extracting peroxy ergosterol from cordyceps cicadae. The method comprises the steps of preparation of crude extract of peroxy ergosterol by an ultrasonic method, enrichment with a lipophilic solvent, separation and purification with a macroporous resin, crystallization and purification and the like. The method adopts ultrasonic extraction, has the advantages of low extraction temperature, short extraction time, high efficiency and the like, the extraction efficiency of peroxy ergosterol is improved, the method also adopts the the macroporous resin which has a good adsorption effect on peroxy ergosterol, and the yield of peroxy ergosterol is increased. The method is simple to operate, the prepared peroxy ergosterol has high purity and high yield, the process stability is good, the production cost is low, the method is suitable for scale production, and has a better applicationprospect.

Description

technical field [0001] The invention relates to the technical field of extraction and separation, in particular to a method for extracting peroxyergosterol from Cordyceps cicadae. Background technique [0002] Cordyceps is an edible and medicinal fungus, which belongs to entomogenous fungi. It is a traditional precious Chinese medicinal material. The whole plant of Cordyceps can be used as medicine. According to modern pharmacological research, it has mainly immunoregulatory effects, anti-tumor effects, analgesic sleep, and mental relief. Stress, promoting blood red blood cell regeneration, improving blood environment, nourishing kidney deficiency, improving diabetic retinopathy and other pharmacological effects. [0003] Research in recent years has shown that Cordyceps has great development potential in the field of biomedicine, especially in functional health food. The active ingredients of Cordyceps cicadae reported in the literature are mainly multiconcincin, ergostero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
CPCC07J71/0005
Inventor 佘志刚黄锡山潘亚宏李闯彪梅秀丽
Owner SUN YAT SEN UNIV
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