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Knitting and coating technology based silk fibroin tissue engineering scaffold and preparation method and application thereof

A technology of tissue engineering scaffold and silk fibroin, which is applied in the field of biomedical materials, can solve the problems of being unable to repair nerve defects, limited therapeutic effect, and poor mechanical properties of the scaffold, so as to alleviate pain and inconvenience of life, and the preparation method is simple and effective , low cost effect

Inactive Publication Date: 2018-05-04
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, tissue engineering scaffolds prepared from regenerated silkworm silk fibroin have poor mechanical properties and cannot be used to repair longer or shorter nerve defects, and the therapeutic effect is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of desericin silkworm silk fibroin

[0028] Weigh about 50 g of original silkworm silk (containing sericin), boil 2 L of double distilled water in a stainless steel pot, add 10 g of sodium carbonate (alkaline solution with anhydrous sodium carbonate concentration of about 0.5%) in the boiling water, and weigh the raw silk Put the silkworm silk into alkaline boiling water, and start timing for 30 minutes from the time when the water boils. During the boiling process, pay attention to the boiling water overflowing, and stir it from time to time with a glass rod. After the first pass of cooking, the silkworm silk is taken out of the water and washed in double distilled water for 3-4 times. Repeat the above operation 3 times, desericin the domestic silkworm silk with 0.5% anhydrous sodium carbonate alkaline solution for 3 times, after the last desericin, wash with double distilled water 3-4 times, soak in double distilled water for 4 hours , and then washed...

Embodiment 2

[0033] Embodiment 2 braiding method and coating method prepare silk fibroin tissue engineering scaffold

[0034] The single desericin silk obtained in Example 1 is wound on different plastic spools respectively, and a plurality of silk threads are woven into a single-layer network structure conduit on a plastic cylindrical inner core by a knitting machine, and the regenerated silk The formic acid solution (10%) of the protein is coated on the catheter, and it is oven-dried at 60°C for 2 hours. After drying, it is placed in 70% ethanol for 2-3 days. After taking it out, it is washed with double distilled water for 1-2 days. The inner core is removed. Finally, the catheter can be dried in an oven at 60°C, and the catheter can be stored for a long time.

Embodiment 3

[0035] Embodiment 3 braiding method and coating method prepare multi-layer silk fibroin tissue engineering scaffold

[0036] The single desericin silk obtained in Example 1 is wound on different plastic spools respectively, and a plurality of silk threads are woven into a conduit with a two-layer network structure on a plastic cylindrical inner core by a knitting machine, and the regenerated silk is The formic acid solution (10%) of the protein is coated on the catheter, and it is oven-dried at 50°C for 2 hours. After drying, it is placed in 70% ethanol for 2-3 days. After taking it out, it is washed with double distilled water for 1-2 days. The inner core is removed. Finally, the catheter can be dried in an oven at 60°C, and the catheter can be stored for a long time.

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Abstract

The invention discloses a knitting and coating technology based silk fibroin tissue engineering scaffold and a preparation method and application of the knitting and coating technology based silk fibroin tissue engineering scaffold, a regenerated silk fibroin layer is adhered between the surface of the scaffold and a silk fiber by coating the silk fibroin tissue engineering scaffold with a regenerated silk fibroin formic acid solution or soaking the silk fibroin tissue engineering scaffold in the regenerated silk fibroin formic acid solution. A material of the silk fibroin tissue engineering scaffold is excellent in mechanical property, bio-safety and degradability, the preparation method is simple, the cost is low, and the preparation method is suitable for industrial volume production.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a silk fibroin tissue engineering scaffold and its preparation method and application. Background technique [0002] Nowadays, nerve injuries caused by traffic accidents, wars, diseases, etc. are increasing year by year. Severe nerve injuries have a devastating impact on the physiology and psychology of the injured, and can lead to loss of sensory and motor functions in patients. As we all know, autologous nerve transplantation is the gold standard surgical treatment, but the sources of autologous nerves are limited, the donor site is permanently denervated, and the allogeneic nerve has immune rejection. Therefore, it is necessary to study artificial nerve grafts to replace autologous nerve grafts. An ideal biomaterial should meet the following conditions: good biocompatibility, good permeability, biodegradability, non-toxicity and processability. [0003] Natural...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/34
CPCA61L27/50A61L27/34A61L2430/32C08L89/00
Inventor 杨宇民张鲁中李贵才赵亚红顾晓松凌珏
Owner NANTONG UNIVERSITY
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