Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of yeast glucan added into albumen powder

A yeast glucan and detection method technology, applied in the field of analytical chemistry, can solve problems such as non-standard detection methods

Inactive Publication Date: 2018-05-01
BEIJING COMPETITOR SPORTS SCI & TECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no standard detection method for yeast β-glucan added in protein powder etc. as food or food ingredients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of yeast glucan added into albumen powder
  • Detection method of yeast glucan added into albumen powder
  • Detection method of yeast glucan added into albumen powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Detection of Yeast Glucan Content

[0037] 1. Liquid chromatography conditions:

[0038] Table 1-1: Chromatographic conditions

[0039]

[0040] 2. Standard solution preparation

[0041] Accurately weigh 0.1320g of glucose standard substance with a ten-thousandth balance, add water to dissolve and transfer to a 100mL volumetric flask, add water to dilute to the mark, and prepare a standard solution stock solution with a concentration of 1.320mg / mL. Store at 4°C.

[0042] 3. Preparation of standard curve

[0043] Accurately pipette 125μL, 250μL, 500μL, 750μL, 1000μL of the standard stock solution, dilute to 1mL with pure water, and prepare the concentrations as 0.165mg / mL, 0.330mg / mL, 0.660mg / mL, 0.990mg / mL, 1.320mg / mL standard solution, thus draw the standard working curve.

[0044] 4. Sample pretreatment

[0045] 4.1 Pretreatment before sample hydrolysis

[0046] Accurately weigh about 5g of the sample (accurate to 0.01g) into a 200mL centrifuge t...

Embodiment 2

[0068] Example 2: Confirmation of linear range

[0069] Obtain the experimental data of standard product according to embodiment 1 detection method, see table 3

[0070] Table 3: Standard test data

[0071]

[0072] Take the standard solution concentration as the ordinate, and the peak area as the abscissa, draw the standard working curve, such as Figure 4 shown.

[0073] Linearity evaluation: the correlation coefficient R2 is 0.9994, so this method is used to indirectly measure the content of yeast glucan, and it shows good linearity between the concentration of 0.165mg / mL and 1.320mg / mL, which is in line with GB / T 27404 "Laboratory Quality The requirements of "Control Specification Food Physical and Chemical Testing" [GB / T 27404 requires correlation coefficient R2≥0.99].

[0074] Figure 4 It is the standard solution linear curve chart drawn according to embodiment 2.

Embodiment 3

[0075] Embodiment 3: precision and stability test

[0076] The glucose standard solution of 1.320mg / mL was repeatedly measured 5 times under the set chromatographic conditions, and the corresponding RSD value was calculated to express the precision of the method. The results are shown in Table 4. Under the same laboratory conditions, the test was repeated 3 times within 3 working days, and the average value and RSD value were checked to characterize the stability of the method by day-to-day precision. The results are shown in Table 5.

[0077] Table 4: Intra-day precision results

[0078]

[0079] Table 5: Interday precision results

[0080]

[0081]

[0082] Conclusion: According to the intra-day precision results in Table 4 and the inter-day precision results in Table 5, it can be seen that the RSD (%) values ​​are all less than 10%, which proves that the method has good precision and stability and meets the requirements of the laboratory.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a detection method of yeast glucan added into albumen powder. The detection method comprises steps of a) drawing a standard curve; b), extracting; c), carrying out hydrolytic treatment on a sample; d), detecting a to-be-detected sample by a chromatographic instrument; e), calculating to obtain a content of the yeast glucan in the to-be-detected sample, and the like. The interference of albumen, fat and a water-soluble saccharide can be eliminated according to the detection method provided by the invention; the content of the yeast glucan can be accurately measured; further, the detection method, through carrying out linear and precision tests and a recovery rate test on a content measurement method of the yeast glucan, all meets requirements of GB / T 27404-2008 Criterion on Quality Control of Laboratories-Chemical Testing of Food, and the detection method is proven to be scientific and effective, and can be used for realizing a quality control purpose to the content measurement of the yeast glucan.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a detection method for yeast glucan added in protein powder. Background technique [0002] After high-intensity acute exercise, the proliferation and differentiation ability and activity of lymphocytes in the body are reduced, and the content and function of immunoglobulin are also affected. Athletes and bodybuilders are more susceptible to diseases, which affects their physical health or competition performance. Therefore, the prevention of immunosuppression after intensive exercise has become an important research topic in sports medicine. At the same time, it is an urgent need for competitive sports and national fitness to find safe and effective sports nutrients or methods to prevent and treat exercise fatigue. [0003] The human immune system is very complex, with thousands of immune cells protecting the body from bacteria, pathogens and other diseases. One of these defe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/06G01N30/86
CPCG01N30/06G01N30/8634
Inventor 李奇庚魏冰曹珍艳
Owner BEIJING COMPETITOR SPORTS SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com