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Oil tea meal polypeptide, and preparation method and application thereof in cryopreservation of bovine semen

A technology of camellia oleifera meal polypeptide and camellia oleifera meal, which is applied in the field of animal husbandry, can solve the problems that have not yet been reported on the in-depth development of hydrolyzed polypeptide of small fruit camellia oleifera meal, and achieve the effect of realizing waste reuse, reducing oxidative damage, and increasing economic added value

Active Publication Date: 2018-04-27
盐城新咏投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the in-depth development of hydrolyzed polypeptides of small fruit tea meal

Method used

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  • Oil tea meal polypeptide, and preparation method and application thereof in cryopreservation of bovine semen
  • Oil tea meal polypeptide, and preparation method and application thereof in cryopreservation of bovine semen
  • Oil tea meal polypeptide, and preparation method and application thereof in cryopreservation of bovine semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of camellia meal polypeptide 1 by trypsin

[0047] Including the following steps:

[0048] Step S1, pulverize the small fruit camellia meal to 80-100 mesh, add water and soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 150g / L;

[0049] Step S2, add trypsin digestion (adding weight is 3.0g / 100g based on meal meal weight), the conditions of digestion are: pH value, 8.0; digestion temperature, 45°C; digestion time, 11 hours; end of digestion Afterwards, treat the trypsin at a high temperature of 90°C for 10 minutes to inactivate the trypsin, cool to room temperature, and let stand to take the supernatant;

[0050] Step S3, filter the supernatant with absorbent cotton 3 times, collect the combined filtrate, and freeze-dry to obtain a crude freeze-dried powder;

[0051] Step S4, the crude freeze-dried powder is dissolved in ultrapure water, and then separated by ultrafiltration membranes with mol...

Embodiment 2

[0052] Example 2: Preparation of camellia meal polypeptide 2 by trypsin

[0053] Including the following steps:

[0054] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water and soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 130g / L;

[0055] Step S2, add trypsin digestion (additional weight is 2.5g / 100g based on meal meal weight), the conditions of digestion are: pH value, 7.8; digestion temperature, 43℃; digestion time, 12 hours; end of digestion Afterwards, treat the trypsin at a high temperature of 90°C for 10 minutes to inactivate the trypsin, cool to room temperature, and let stand to take the supernatant;

[0056] Step S3, filter the supernatant with absorbent cotton 3 times, collect the combined filtrate, and freeze-dry to obtain a crude freeze-dried powder;

[0057] Step S4, the crude freeze-dried powder is dissolved in ultrapure water, and then separated by ultrafiltration membranes with m...

Embodiment 3

[0058] Example 3: Preparation of Camellia Cake Polypeptide 3 by Trypsin

[0059] Including the following steps:

[0060] Step S1, pulverize the small fruit oil tea meal to 80-100 mesh, add water and soak overnight, and finally make a meal powder suspension, the mass concentration of the meal powder suspension is 170g / L;

[0061] Step S2, add trypsin digestion (additional weight is 3.5g / 100g based on meal meal weight), and digestion conditions are: pH value, 8.2; digestion temperature, 47°C; digestion time, 10 hours; end of digestion Afterwards, treat the trypsin at a high temperature of 90°C for 10 minutes to inactivate the trypsin, cool to room temperature, and let stand to take the supernatant;

[0062] Step S3, filter the supernatant with absorbent cotton 3 times, collect the combined filtrate, and freeze-dry to obtain a crude freeze-dried powder;

[0063] Step S4, the crude freeze-dried powder is dissolved in ultrapure water, and then separated by ultrafiltration membranes with mol...

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Abstract

The invention discloses an oil tea meal polypeptide, and a preparation method and an application thereof in cryopreservation of bovine semen. The oil tea meal hydrolytic polypeptide has a variety of activities, and the activities of the hydrolytic polypeptide are affected by proteolytic sites in the oil tea meal: the polypeptide produced by trypsin hydrolysis and having the molecular weight of [5000 u, 3000 u] has the effect of improving the antioxidant ability of the bovine cryopreserved semen, and can be used as a bovine semen cryopreservation protection agent for reducing oxidative damage;and the polypeptide produced by alkaline protease hydrolysis and having the molecular weight of [5000 u, 3000 u] can promote empathema and pregnancy of anestrus cows. Moreover, the preparation methodof the hydrolytic polypeptide is simple, only simple crushing, enzymatic hydrolysis, ultrafiltration and freeze drying are used, and all the steps can be realized in a factory with mass production andare easy to implement. Waste reuse of camellia meiocarpa hu. meal is realized, and the economic added value of the camellia meiocarpa hu. meal can be increased.

Description

Technical field [0001] The invention belongs to the field of animal husbandry, and relates to a camellia meal polypeptide, a preparation method and application in the aspect of frozen preservation of bovine semen. Background technique [0002] Camellia meiocarpa Hu. (Camellia meiocarpa Hu.) is the main planted species in Camellia genus in my country, which is second only to the common Camellia oleifer (Camellia oleifer aAbel.) in terms of cultivation area and annual output. , Hunan, Guizhou and other places. Xiaoguo Camellia is also known as Jiangxizi, Xiaocha, Jixinzi, Xiaoye Camellia, and sheep shit. The fruit is small, the leaves are small, the buds are small, and the buds and bracts are not hairy. [0003] Camellia oleifera meal is a by-product obtained by extracting oil from Camellia oleifera. Although there are already tea farms that detoxify these camellia meal as animal feed, due to the immature process, most of the tea camellia meal is still thrown away as waste or compo...

Claims

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Application Information

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IPC IPC(8): C07K2/00C12P21/06C07K1/34A01N1/02
CPCA01N1/0226C07K2/00C12P21/06
Inventor 盛蓓蓓李沛王崇智
Owner 盐城新咏投资发展有限公司
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